Search results for "ELISA Western Blot Reagents"

WB (Western Blot) (Anti-HA Tag mouse monoclonal antibody at 1:1000 dilutionLane A: HA-GST (Recombinant protein)(30ng)Lane B: GST-HA (Recombinant protein)(10ng)Lane C: HA-ARG1-myc transfected 293 cell lysate (2ug)Lane D: HA-mFABP4-myc transfected 293 cell lysate (2ug)Lane E: myc-mFABP4-HA transfected 293 cell lysate (0.5ug)Lane F: myc-ARG1-HA transfected 293 cell lysate (2ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

IP (Immunoprecipitation) (Anti-HA Tag was immunoprecipitated using:Lane A: HA-ARG1-myc transfected 293 cell lysate (0.5mg)Lane B: myc-ARG1-HA transfected 293 cell lysate (0.5mg)2 uL anti-HA Tag mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-HA Tag rabbit monoclonal antibody, at 1:100 dilutionSecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.)

IF (Immunofluorescence) (Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D). Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-HA-Tag monoclonal antibody at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).)

IF (Immunofluorescence) (Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (L) or mock-transfected (R). Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SBI Mouse anti-HA-tag monoclonal antibody at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green).)

FCM (Flow Cytometry) (Profile of anti-HA Tag reactivity on 293 transfected cells analyzed by flow cytometry. 293 cells transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D) were fixed and permeabilized according to the manufacturer's manual and subsequently stained with Purified Mouse Anti-HA Tag antibody Or positive control antibody for 30 min on ice. Cells were washed twice and incubated with 1 ug of a FITC Goat Anti-Mouse Ig secondary antibody for 30 min on ice. Cells were washed twice and analyzed by flow cytometry.)

Application Data (This antibody can be used at 0.5-1 ug/mL with the appropriate secondary reagents to detect HA-tagged protein (amino-terminus HA tag or carboxy-terminus HA tag).)

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2 results for "ELISA Western Blot Reagents" - showing 1-2

HA, Monoclonal Antibody (Cat# AAA27712)

Mouse Anti-Human IgG (Fc) (gamma chain specific), Monoclonal Secondary Antibody (Cat# AAA14898)

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