Search results for " Spindle pole"

10 results for " Spindle pole" - showing 1-10

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IHC (Immunohistchemistry) (At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

PRC1, Polyclonal Antibody (Cat# AAA31449)

Full Name

Phospho-PRC1 (Thr470) Antibody

Gene Names

PRC1; ASE1

Reactivity

Human, Mouse
Predicted Reactivity: Pig (100%), Horse (89%), Sheep (89%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

HSF1, Polyclonal Antibody (Cat# AAA31391)

Full Name

Phospho-HSF1 (Ser307) Antibody

Gene Names

HSF1; HSTF1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Bamacan, Polyclonal Antibody (Cat# AAA31455)

Full Name

Phospho-Bamacan (Ser1083) Antibody

Gene Names

SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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TOPBP1, Polyclonal Antibody (Cat# AAA31285)

Full Name

Phospho-TOPBP1 (Ser1138) Antibody

Gene Names

TOPBP1; TOP2BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

TOPBP1, Polyclonal Antibody (Cat# AAA31467)

Full Name

Phospho-TOPBP1 (Ser1159) Antibody

Gene Names

TOPBP1; TOP2BP1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (AAA31033 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CDC25B, Polyclonal Antibody (Cat# AAA31033)

Full Name

Phospho-CDC25B (Ser323) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

Nuclear Mitotic Apparatus Protein (NuMA), Monoclonal Antibody (Cat# AAA13819)

Full Name

Nuclear Mitotic Apparatus Protein (NuMA) Mouse Monoclonal Antibody

Gene Names

NUMA1; NUMA; NMP-22

Reactivity

Human (Others not known)

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Pricing

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PRC1, Polyclonal Antibody (Cat# AAA31450)

Full Name

Phospho-PRC1 (Thr481) Antibody

Gene Names

PRC1; ASE1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (80%), Horse (90%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

Immunohistochemistry, Western Blot

Pricing

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TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

Full Name

RAT ANTI TUBULIN ALPHA

Applications

Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot

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10 results for " Spindle pole" - showing 1-10

PRC1, Polyclonal Antibody (Cat# AAA31449)

HSF1, Polyclonal Antibody (Cat# AAA31391)

Bamacan, Polyclonal Antibody (Cat# AAA31455)

TOPBP1, Polyclonal Antibody (Cat# AAA31285)

TOPBP1, Polyclonal Antibody (Cat# AAA31467)

CDC25B, Polyclonal Antibody (Cat# AAA31033)

Nuclear Mitotic Apparatus Protein (NuMA), Monoclonal Antibody (Cat# AAA13819)

PRC1, Polyclonal Antibody (Cat# AAA31450)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

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Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

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Clone

10 results for " Spindle pole" - showing 1-10

PRC1, Polyclonal Antibody (Cat# AAA31449)

HSF1, Polyclonal Antibody (Cat# AAA31391)

Bamacan, Polyclonal Antibody (Cat# AAA31455)

TOPBP1, Polyclonal Antibody (Cat# AAA31285)

TOPBP1, Polyclonal Antibody (Cat# AAA31467)

CDC25B, Polyclonal Antibody (Cat# AAA31033)

Nuclear Mitotic Apparatus Protein (NuMA), Monoclonal Antibody (Cat# AAA13819)

PRC1, Polyclonal Antibody (Cat# AAA31450)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

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