Search results for " Retroviral Expression"

1 results for " Retroviral Expression" - showing 1-1

Display

< >

<< Previous Next >>

IP (Immunoprecipitation) (Figure 13 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)

WB (Western Blot) (Figure 12 KD Validation in Chondrocytes (Ahmad et al., 2009)Chondrocytes were transfected with either MyD88 siRNA or control siRNA and analyzed for MyD88 expression by immunoblotting with anti-Myd88 antibodies that confirmed inhibition of the target proteins.)

WB (Western Blot) (Figure 11 KO Validation in IECs (Vlantis et al., 2014)Western blot with anti-MyD88 antibodies on intestinal epithelial cells (IECs) showing efficient deletion of MyD88 and concomitant expression of GFP in MyD88IEC-KO mice, but not in MyD88 knockout mice.)

WB (Western Blot) (Figure 10 KO Validation in MyD88-deficient MEF cell line (Burns et al., 2003)MyD88?/?-deficient MEF cell line was reconstituted by retroviral infection with an empty vector, MyD88, or MyD88s expression vectors. The levels of MyD88 (isoform 1) or MyD88s (isoform 3) were confirmed with anti-MyD88 antibodies and MyD88 expression was not detected in the MyD88-deficient cells.)

WB (Western Blot) (Figure 9 KO Validation in Macrophages (Miller et al., 2006)Bone marrow-derived macrophages from wild type (WT) mice and MyD88 knockout mice were assessed for MyD88 protein expression by anti-MyD88 antibodies. MyD88 expression was detected in WT mice, but not in MyD88 knockout mice.)

IF (Immunofluorescence) (Figure 8 Immunofluorescence Validation of MyD88 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling MyD88 with 2125 at 10 μg/mL, followed by Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IHC (Immunohistochemistry) (Figure 7 Immunohistochemistry Validation of MyD88Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-MyD88 antibody (2125) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IF (Immunofluorescence) (Figure 6 Immunofluorescence Validation of MyD88Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MyD88 with 2125 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing nucleus staining on human testis cells.)

WB (Western Blot) (Figure 5 Validation with MyD88 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or MyD88 siRNAs (lane 2)Loading: 10 μg of HeLa whole cell lysates per lane.Antibodies: 2125 (2 μg /mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 4 Animal Species ReactivityLoading: Lysates/proteins at 15 μg per lane.Antibodies: 2125 (2 μg/mL) or 2127 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 μg of lysates per lane.Antibodies: MyD88 2125 (2 μg/mL), MyD88 2127 (2 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: MyD88 2125 (2 μg/mL), MyD88 2127 (2 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 1 Western Blot Validation of MyD88 in HeLa (A) and Jurket (B) CellsLoading: 15 μg of lysates per lane.Antibodies: 2125 (1 μg/mL) 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

MYD88, Polyclonal Antibody (Cat# AAA10930)

Full Name

MYD88 Antibody

Gene Names

MYD88; MYD88D

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

MYD88 Antibody is affinity chromatography purified via peptide column.

Pricing

Loading...

Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

1 results for " Retroviral Expression" - showing 1-1

MYD88, Polyclonal Antibody (Cat# AAA10930)

Loading...

Need a datasheet, manual, COA, or MSDS? Have a question?

Interested to get a quote about our products?

Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

1 results for " Retroviral Expression" - showing 1-1

MYD88, Polyclonal Antibody (Cat# AAA10930)

Submit a Question

Request more Information

Request a Manual

Request a Quote