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29 results for " RIPA Buffer" - showing 1-29
IP (Immunoprecipitation)
(IP/WB analysis of mouse brain, rat brain membrane lysate and mouse brain membrane lysate, were resolved by electrophoresis, transferred to PVDF membrane and probed using AAA14705 (1 :500). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein AMYLOID OLIGOMER (-80kD).)
b-Amyloid, Oligomer A11, Polyclonal Antibody (Cat# AAA14705)
Full Name
b-Amyloid, Oligomer A11
Reactivity
Human, Mouse, Rat and Eukaryote
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
Serum
Pricing
WB (Western Blot)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Orexin receptor 1, Polyclonal Antibody (Cat# AAA13669)
Full Name
Goat anti-Orexin receptor 1 Antibody
Gene Names
HCRTR1; OX1R
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Sheep
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
GAPDH, Polyclonal Antibody (Cat# AAA13657)
Full Name
Goat anti-GAPDH (Internal) Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistchemistry)
((3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
KCNN4/KCa3.1, Antibody (Cat# AAA13678)
Full Name
Goat Anti-KCNN4/KCa3.1 Antibody
Gene Names
KCNN4; IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
((0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
secretogranin II, Antibody (Cat# AAA13675)
Full Name
Goat anti-secretogranin II (aa203-217) Antibody
Gene Names
SCG2; SN; CHGC; EM66; SgII
Reactivity
Tested: Human
Expected from sequence similarity: Human, Dog, Pig
Expected from sequence similarity: Human, Dog, Pig
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
((1ug/ml) staining of Mouse Kidney lysate (A) and of Mouse Spleen lysate (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
PBR, Polyclonal Antibody (Cat# AAA13662)
Full Name
Goat anti-PBR (mouse) Antibody
Gene Names
Tspo; IBP; PBR; Bzrp; Tspo1
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistchemistry)
((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.)
EDF1/MBF1, Antibody (Cat# AAA13677)
Full Name
Goat Anti-EDF1/MBF1 Antibody
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Flow Cytometry
Pricing
WB (Western Blot)
((0.5ug/ml) staining of A549 (A), HepG2 (B) and K562 (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
TNFRSF1A/TNFR1, Polyclonal Antibody (Cat# AAA13672)
Full Name
Goat anti-TNFRSF1A/TNFR1 Antibody
Gene Names
TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat, Pig
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
((0. 03ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
CLOCK, Antibody (Cat# AAA13674)
Full Name
Goat anti-CLOCK (aa458-472) Antibody
Gene Names
CLOCK; KAT13D; bHLHe8
Reactivity
Tested: Mouse, Rat
Expected from sequence similarity: Human, Mouse, Cow
Expected from sequence similarity: Human, Mouse, Cow
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Western Blot analysis of human muscle lysate (35ug protein in RIPAbuffer) using AAA24036 (0.01ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
COX4I1, Polyclonal Antibody (Cat# AAA24036)
Full Name
COX4I1, CT (Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial, Cytochrome C Oxidase Polypeptide IV, Cytochrome C Oxidase Subunit IV Isoform 1, COX IV-1, COX IV-1)
Gene Names
COX4I1; COX4; COXIV; COX4-1
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
Amyloid beta peptide, Polyclonal Antibody (Cat# AAA13671)
Full Name
Goat anti-Amyloid beta peptide (aa1-16) Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow
Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Antibody (0.5 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
AIF1 / IBA1, Polyclonal Antibody (Cat# AAA12318)
Full Name
Goat Polyclonal to Human AIF1 / IBA1
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human, Monkey, Mouse, Rat, Hamster, Pig
Applications
Immunohistochemistry, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
((0.3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
GPX4, Polyclonal Antibody (Cat# AAA13650)
Full Name
Goat anti-GPX4 (Isoform a and c) Antibody
Gene Names
GPX4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
Reactivity
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(AAA13663 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
P2RX7/P2X7 receptor, Polyclonal Antibody (Cat# AAA13663)
Full Name
Goat anti-P2RX7/P2X7 receptor Antibody
Gene Names
P2RX7; P2X7
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistchemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
c-FOS, Antibody (Cat# AAA13673)
Full Name
Goat anti-c-FOS (aa283-295) Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
ROBO1, Polyclonal Antibody (Cat# AAA12322)
Full Name
Goat Polyclonal to Human ROBO1
Gene Names
ROBO1; SAX3; DUTT1
Reactivity
Bat, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Pig, Human, Monkey, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
WB (Western Blot)
((2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
CLEC16A, Polyclonal Antibody (Cat# AAA13667)
Full Name
Goat anti-CLEC16A (aa1040-53) Antibody
Gene Names
CLEC16A; Gop-1; KIAA0350
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IF (Immunofluorescence)
((5ug/ml) staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes.)
NANOG, Polyclonal Antibody (Cat# AAA13647)
Full Name
Goat anti-NANOG Antibody
Reactivity
Tested: Human, Pig; Expected from sequence similarity: Human, Dog, Pig
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
PNPLA3/Adiponutrin, Polyclonal Antibody (Cat# AAA13661)
Full Name
Goat anti-PNPLA3/Adiponutrin Antibody
Gene Names
Pnpla3; Adpn
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.)
Doublecortin/DCX, Polyclonal Antibody (Cat# AAA13668)
Full Name
Goat anti-Doublecortin/DCX (aa232-242) Antibody
Gene Names
DCX; DC; DBCN; LISX; SCLH; XLIS
Reactivity
Species Reactivity: Mouse, Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Argininosuccinate synthetase 1, Polyclonal Antibody (Cat# AAA13660)
Full Name
Goat anti-Argininosuccinate synthetase 1 Antibody
Gene Names
ASS1; ASS; CTLN1
Reactivity
Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
GFAP, Antibody (Cat# AAA13651)
Full Name
Goat anti-GFAP Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IS (Immunostaining)
(Immunostaining of 25?m thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval withcitrate buffer Ph 6 at 80C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.)
Androgen Receptor, Polyclonal Antibody (Cat# AAA13656)
Full Name
Goat anti-Androgen Receptor Antibody
Gene Names
AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(AAA13655 Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.)
CCKBR, Polyclonal Antibody (Cat# AAA13655)
Full Name
Goat anti-CCKBR Antibody
Gene Names
CCKBR; GASR; CCK-B; CCK2R
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow
Expected from sequence similarity: Human, Mouse, Rat, Cow
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
FCM (Flow Cytometry)
(AAA13670 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
NRF2, Polyclonal Antibody (Cat# AAA13670)
Full Name
Goat anti-NRF2 (aa445-458) Antibody
Gene Names
NFE2L2; NRF2; HEBP1
Reactivity
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
FCM (Flow Cytometry)
(AAA13649 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Arrestin beta 2, Antibody (Cat# AAA13649)
Full Name
Goat anti-Arrestin beta 2 Antibody
Gene Names
ARRB2; ARB2; ARR2; BARR2
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(AAA13658 (0.5 ug/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35 ug protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence. Data obtained from anonymous customer)
IRAK3, Polyclonal Antibody (Cat# AAA13658)
Full Name
Goat anti-IRAK3 (mouse) Antibody
Gene Names
Irak3; IRAK-M; AI563835; 4833428C18Rik
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody)
MYD88, Polyclonal Antibody (Cat# AAA13652)
Full Name
Goat anti-MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix™ coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
AIF1/IBA1, Polyclonal Antibody (Cat# AAA13659)
Full Name
Goat anti-AIF1/IBA1 (isoform 3) Antibody
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog
Expected from sequence similarity: Human, Mouse, Dog
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing