Search results for " Pyruvate Kinase pk "

7 results for " Pyruvate Kinase pk " - showing 1-7

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SDS-PAGE

PKM2, Active Protein (Cat# AAA11792)

Full Name

PKM2, 1-531aa, Human, His tag, E Coli (Bioactivity Validated)

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

Standard Curve (Sample)

Pyruvate Kinase (M2-PK), ELISA Kit (Cat# AAA12400)

Full Name

Mouse Pyruvate Kinase (M2-PK) ELISA Kit

Reactivity

Mouse

Pricing

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IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistchemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistchemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000, 1:512000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)

WB (Western Blot) (Western Blot Positive WB detected in: MCF-7 whole cell lysate at 40ug, 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)

WB (Western Blot) (Western Blot Positive WB detected in: Mouse Brain tissue, Mouse Skeldtal Muscle tissue All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)

WB (Western Blot) (Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1min)

PKM, Monoclonal Antibody (Cat# AAA27048)

Full Name

PKM Monoclonal Antibody

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation

Purity

>95%, Protein G purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using PKM1-specific Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using PKM1-specific Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using PKM1-specific Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using PKM1-specific Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PKM1-specific antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)

PKM1-specific, Polyclonal Antibody (Cat# AAA28375)

Full Name

PKM1-specific Rabbit pAb

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

Standard Curve (Sample)

Pyruvate Kinase, Muscle (PKM2), ELISA Kit (Cat# AAA13913)

Full Name

Human Pyruvate Kinase, Muscle (PKM2) ELISA Kit

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

Reactivity

Human

Pricing

Standard Curve (Sample)

pyruvate kinase, muscle, ELISA Kit (Cat# AAA14969)

Full Name

Human Pyruvate Kinase, M2-PK ELISA Kit

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30

Reactivity

Human

Pricing

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FCM (Flow Cytometry) (Overlay histogram showing HepG2 cells stained with AAA27041 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with AAA27041 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

IP (Immunoprecipitation) (Immunoprecipitating PKM in Hela whole cell lysateLane 1: Mouse control IgG instead of AAA27041 in Hela whole cell lysate.Lane 2: AAA27041 (1ul) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug)For western blotting, the blot was detected with AAA27041 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000)

IF (Immunofluorescence) (Immunofluorescence staining of HepG2 cells with AAA27041 at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)

IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with AAA27041 at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)

IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with AAA27041 at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)

IHC (Immunohistochemistry) (IHC image of AAA27041 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image of AAA27041 diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistchemistry) (IHC image of AAA27041 diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image of AAA27041 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistchemistry) (IHC image of AAA27041 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot) (Western Blot: Positive WB detected in: MCF-7 whole cell lysateAll lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 58 kDaObserved band size: 58 KDaExposure time: 5min)

WB (Western Blot) (Western Blot: Positive WB detected in: MCF-7 whole cell lysate at 40µg, 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg, 0.3125µgAll lanes: PKM antibody at 1:4000Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 58 kDaObserved band size: 58 KDaExposure time: 5min)

WB (Western Blot) (Western Blot: Positive WB detected in: Rat Heart tissue, Rat Spleen tissue, Rat Brain tissueAll lanes: PKM antibody at 1:4000Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 55-60 kDaObserved band size: 55-60 kDa)

WB (Western Blot) (Western Blot: Positive WB detected in: Mouse Heart tissue, Mouse Brain tissue, Mouse Skeletal Muscle tissueAll lanes: PKM antibody at 1:4000Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 58 kDaObserved band size: 58 KDa)

WB (Western Blot) (Western Blot: Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysateAll lanes: PKM antibody at 1:4000Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 58 kDaObserved band size: 58 KDaExposure time: 1min)

PKM, Monoclonal Antibody (Cat# AAA27041)

Full Name

PKM Monoclonal Antibody

Reactivity

Human, Rat, Mouse, Rabbit

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation

Purity

>95%, Protein G purified

Pricing

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7 results for " Pyruvate Kinase pk " - showing 1-7

PKM2, Active Protein (Cat# AAA11792)

Pyruvate Kinase (M2-PK), ELISA Kit (Cat# AAA12400)

PKM, Monoclonal Antibody (Cat# AAA27048)

PKM1-specific, Polyclonal Antibody (Cat# AAA28375)

Pyruvate Kinase, Muscle (PKM2), ELISA Kit (Cat# AAA13913)

pyruvate kinase, muscle, ELISA Kit (Cat# AAA14969)

PKM, Monoclonal Antibody (Cat# AAA27041)

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7 results for " Pyruvate Kinase pk " - showing 1-7

PKM2, Active Protein (Cat# AAA11792)

Pyruvate Kinase (M2-PK), ELISA Kit (Cat# AAA12400)

PKM, Monoclonal Antibody (Cat# AAA27048)

PKM1-specific, Polyclonal Antibody (Cat# AAA28375)

Pyruvate Kinase, Muscle (PKM2), ELISA Kit (Cat# AAA13913)

pyruvate kinase, muscle, ELISA Kit (Cat# AAA14969)

PKM, Monoclonal Antibody (Cat# AAA27041)

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