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2 results for " Lipids Lipoproteins" - showing 1-2
Application Data
(All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.)
GBA/Glucosylceramidase, Monoclonal Antibody (Cat# AAA11697)
Full Name
Anti-GBA/Glucosylceramidase Rabbit Monoclonal Antibody
Gene Names
GBA; GCB; GBA1; GLUC
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
WB (Western Blot)
(Western blot analysis of Lipoprotein lipase expression in Human fetal liver lysate (AAA11694).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPL monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LPL)
Lipoprotein lipase, Monoclonal Antibody (Cat# AAA11694)
Full Name
Anti-Lipoprotein lipase Rabbit Monoclonal Antibody
Gene Names
LPL; LIPD; HDLCQ11
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing