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11 results for " Intracellular Signaling" - showing 1-11
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
AKT1, Polyclonal Antibody (Cat# AAA31364)
Full Name
Phospho-AKT1 (Ser124) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using APP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
APP, Polyclonal Antibody (Cat# AAA28326)
Full Name
APP Rabbit pAb
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
JIP1, Polyclonal Antibody (Cat# AAA31317)
Full Name
Phospho-JIP1 (Thr205) Antibody
Gene Names
MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Jak2, Polyclonal Antibody (Cat# AAA31311)
Full Name
Phospho-Jak2 (Tyr1008) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
AR, Polyclonal Antibody (Cat# AAA31420)
Full Name
Phospho-AR (Tyr535) Antibody
Gene Names
AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (92%), Bovine (85%), Sheep (85%), Rabbit (92%), Dog (92%)
Predicted Reactivity: Pig (92%), Bovine (85%), Sheep (85%), Rabbit (92%), Dog (92%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer imageSLAMF3 blockade in human hepatocytes is associated with lower susceptibility to HCV. (A) SLAMF3 was stained in primary human hepatocytes (PHHs) and cells from the Huh-7 human hepatoma cell line with a specific antibody (HLy9.1.25 clone; grey) and an isotype-matched control (empty). One of four independent experiments is shown. Huh-7 cells were transfected with scrambled control (sc) siRNA or three specific siRNAs (#1, #2 and #3) targeting SLAMF3, prior to infection with HCVcc; siRNA efficiency was checked by quantifying SLAMF3 mRNA (B) and the CD81 expression level (C) by flow cytometry analysis at 48 h post-transfection. Results are presented as the mean +/-SD (n = 3). Intracellular viral RNA was quantified at 72 h p.i. (D) and the infection was measured at 72 h p.i. by focus-forming units FFUs counting (E) (as inhibition percent; mean of three independent experiments; error bars: SD. **p)
CD229, Monoclonal Antibody (Cat# AAA11951)
Full Name
MOUSE ANTI HUMAN CD229
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Applications
Flow Cytometry, Immunoprecipitation
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
JIP1, Polyclonal Antibody (Cat# AAA31318)
Full Name
Phospho-JIP1 (Thr103) Antibody
Gene Names
MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)
Full Name
Anti-Musashi 1/Msi1 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
CDw17, Monoclonal Antibody (Cat# AAA13832)
Full Name
CDw17 (Lactosylceramide or LacCer) Mouse Monoclonal Antibody
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
CD19, Polyclonal Antibody (Cat# AAA28685)
Full Name
CD19 Antibody (N-term)
Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing