Search results for " Hematopoietic Progenitors"

4 results for " Hematopoietic Progenitors" - showing 1-4

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IF (Immunofluorescence) (Immunofluorescence analysis of Raw264.7 cells using CD44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells using CD44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CD44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using CD44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CD44 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using CD44 antibody at dilution of 1:100 (40x lens).)

CD44, Polyclonal Antibody (Cat# AAA28336)

Full Name

CD44 Rabbit pAb

Gene Names

CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

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SDS-PAGE (SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

CD34, Monoclonal Antibody (Cat# AAA23910)

Full Name

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Reactivity

Human, Rat. Others not known.

Applications

Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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SDS-PAGE (SDS-PAGE Analysis Purified Podocalyxin Mouse Monoclonal Antibody (3D3). Confirmation of Integrity and Purity of Antibody.)

IF (Immunofluorescence) (Confocal Immunofluorescence of HeLa cells using Podocalyxin Mouse Monoclonal Antibody (3D3) labeled with CF488 (Green); Reddot is used to label the nuclei.)

WB (Western Blot) (Western Blot Analysis of HeLa cell lysate using Podocalyxin Mouse Monoclonal Antibody (3D3).)

FCM (Flow Cytometry) (Flow Cytometry of NCCIT Cells using Podocalyxin Monoclonal Antibody (3D3).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Monoclonal Antibody (3D3).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (3D3).)

Podocalyxin (PODXL), Monoclonal Antibody (Cat# AAA13811)

Full Name

Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Mouse Monoclonal Antibody

Gene Names

PODXL; PC; PCLP; Gp200; PCLP-1

Reactivity

Human, Rabbit, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry

Pricing

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Application Data (Phycoerythrin conjugated Mouse anti Human CD45 antibody, clone F10-89-4 used to evaluate CD45 expression on bone-marrow derived mesenchymal stem cells by flow cytometryImage caption:From: Chaturvedi CP, Tripathy NK, Minocha E, Sharma A, Rahman K, Nityanand S.Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.Stem Cells Int. 2018 Oct 17;2018:6901761.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (FFPE human tonsil stained with Mouse Anti-Human CD45 , biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)

Application Data (Alexa Fluor A647 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 used for the identification of CD45 expressing cells in blood samples by immunofluorescence.Image caption:Representative CTCs found in Patients 1–5. Each column represents one patient's CTCs: Red = cytokeratin, Green = CD45, and Blue = DAPI.From: Marrinucci D, Bethel K, Lazar D, Fisher J, Huynh E, Clark P, Bruce R, Nieva J, Kuhn P.Cytomorphology of circulating colorectal tumor cells: a small case series.J Oncol. 2010:861341.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Alexa Fluor A647 conjugated Mouse anti Human CD45 antibody, clone F10-89-4 used for the identification of CD45 expressing cells in blood samples by immunofluorescence.Image caption:Relocation and characterization of CTCs using the Wright-Giemsa stain. After initial identification of CTCs via fluorescent images, subsequent Wright-Giemsa stain and/or Papanicolau stain of these cells is performed: (a) composite 3-color fluorescent image, (b) DAPI channel only, (c) cytokeratin channel only, (d) CD45 channel only, and (e) corresponding Wright-Giemsa-stained cell.From: Marrinucci D, Bethel K, Lazar D, Fisher J, Huynh E, Clark P, Bruce R, Nieva J, Kuhn P.Cytomorphology of circulating colorectal tumor cells: a small case series.J Oncol. 2010:861341.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human CD45 antibody, clone F10-89-4 used for the evaluation of CD45 expression on brain derived mesenchymal stem cells.Image caption:Characterisation of clonal perivascular MSC progenitor lines. (A) Single cell in vitro a few hours after plating, and culture at 7 and 21 days, respectively. Scale bar 50 µm. (B) Clonally derived MSC show the same antigen profile as detected for polyclonal lines with pericyte and MSC markers but neither neuronal nor glial precursor cell markers (O4, GFAP) (example here cortical clone; green = isotype, red = respective marker).(C) QPCR comparing clones derived from the ventricular zone (n = 2), and the cortex (n = 2) to a human neuronal progenitor cell line MesC2.10. Data show the relative higher expression of mRNA for RGS5, PDGFR-? and ?-SMA in the MSC clones compared to the control. As expected, Nestin mRNA was present in perivascular MSC clones and neural progenitors at comparable amounts. NG2 mRNA was slightly higher expressed in the MSC clones compared to the control. (D) Importantly, neural progenitors markers such as CD133, SOX1, NGN2, PAX6 and Musashi were highly expressed in the positive control but not detectable in MSC clones.From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012)The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.PLoS ONE 7(4): e35577.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human CD45 antibody, clone F10-89-4 used for the evaluation of CD45 expression on brain derived mesenchymal stem cells.Image caption:Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors. (A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-?, RGS5, CD146, Nestin, ?-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012) The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.PLoS ONE 7(4): e35577.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human CD45 antibody, clone F10-89-4 used for the evaluation of CD45 expression on brain derived mesenchymal stem cells.Image caption:FACS sorting of brain-derived progenitor cell lines. Human progenitor cells were positively sorted for the MSC markers CD105 and CD13, and thereafter negatively for hematopoietic (CD45) and endothelial markers (CD31). Histogram illustrates results (A, B) for the two different cortical lines and (C, D) for the two lines from the ventricular zone. 99.4–100% of the cell population expressed both, CD105 and CD13, and cultures did not contain endothelial or hematopoietic cells.From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012) The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.PLoS ONE 7(4): e35577.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Direction of Mouse anti Human CD45 in an immunohistochemical analysis of an acetone fixed, frozen human colon section using the Histar Detection Kit .)

Application Data (Figure A. FITC conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse anti Human CD45 . All experiments performed on red cell lysed human peripheral blood gated on leukocytes in the presence of Human SeroBlock .)

Application Data (Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control . Figure B . Alexa Fluor 488 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse anti Human CD45 . All experiments performed on Human blood gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)

CD45, Monoclonal Antibody (Cat# AAA12270)

Full Name

Mouse Anti Human CD45: Amethyst Orange

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

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Reactivity

Gene Name

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Clone

4 results for " Hematopoietic Progenitors" - showing 1-4

CD44, Polyclonal Antibody (Cat# AAA28336)

CD34, Monoclonal Antibody (Cat# AAA23910)

Podocalyxin (PODXL), Monoclonal Antibody (Cat# AAA13811)

CD45, Monoclonal Antibody (Cat# AAA12270)

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4 results for " Hematopoietic Progenitors" - showing 1-4

CD44, Polyclonal Antibody (Cat# AAA28336)

CD34, Monoclonal Antibody (Cat# AAA23910)

Podocalyxin (PODXL), Monoclonal Antibody (Cat# AAA13811)

CD45, Monoclonal Antibody (Cat# AAA12270)

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