Search results for " G1 S Checkpoint"

4 results for " G1 S Checkpoint" - showing 1-4

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain using CDK6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CDK6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using CDK6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of H9C2 cells using CDK6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using CDK6 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophageal using CDK6 Rabbit pAb at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CDK6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

CDK6, Polyclonal Antibody (Cat# AAA28330)

Full Name

CDK6 Rabbit pAb

Gene Names

CDK6; PLSTIRE

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CDK4 antibody . Western blot was performed from the immunoprecipitate using CDK4 antibody at a dilition of 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using CDK4 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using CDK4 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using CDK4 Rabbit pAb at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and CDK4 knockout (KO) HeLa cells, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

CDK4, Polyclonal Antibody (Cat# AAA28337)

Full Name

CDK4 Rabbit pAb

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Cyclin D1 antibody . Western blot was performed from the immunoprecipitate using Cyclin D1 antibody at a dilition of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.)

Cyclin D1, Monoclonal Antibody (Cat# AAA28377)

Full Name

[KO Validated] Cyclin D1 Rabbit mAb

Gene Names

CCND1; BCL1; PRAD1; U21B31; D11S287E

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistchemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Bub1, Polyclonal Antibody (Cat# AAA31312)

Full Name

Phospho-Bub1 (Ser459) Antibody

Gene Names

BUB1; BUB1A; BUB1L; hBUB1

Reactivity

Human, Rat

Applications

IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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4 results for " G1 S Checkpoint" - showing 1-4

CDK6, Polyclonal Antibody (Cat# AAA28330)

CDK4, Polyclonal Antibody (Cat# AAA28337)

Cyclin D1, Monoclonal Antibody (Cat# AAA28377)

Bub1, Polyclonal Antibody (Cat# AAA31312)

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4 results for " G1 S Checkpoint" - showing 1-4

CDK6, Polyclonal Antibody (Cat# AAA28330)

CDK4, Polyclonal Antibody (Cat# AAA28337)

Cyclin D1, Monoclonal Antibody (Cat# AAA28377)

Bub1, Polyclonal Antibody (Cat# AAA31312)

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