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18 results for " Endothelium" - showing 1-18


product-image-AAA13801_WB7.jpg WB (Western Blot) (Western Blot Analysis of human Jurkat cell lysate using CD31 Mouse Monoclonal Antibody (C31.7).)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13801)

Full Name
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Cynomolgus Monkey, Rabbit.
Weakly reacts with Rat
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
product-image-AAA11696_WB.jpg WB (Western Blot) (Western blot analysis of Podoplanin expression in human placenta lysate (AAA11696).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDPN)

Podoplanin, Monoclonal Antibody (Cat# AAA11696)

Full Name
Anti-Podoplanin Rabbit Monoclonal Antibody tested for WB in Human, Mouse, Rat.
Gene Names
PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-chromatography
Pricing
product-image-AAA11586_IHC3.jpg IHC (Immunohistochemistry) (Anti-VE Cadherin antibody, AAA11586, IHC(P)IHC(P): Rat Brain Tissue)

VE Cadherin, Polyclonal Antibody (Cat# AAA11586)

Full Name
Polyclonal Anti-CDH5 Antibody; Anti-VE Cadherin antibody
Gene Names
CDH5; 7B4; CD144
Reactivity
Human, Rat; Predicted: Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA30270_FCM6.jpg FCM (Flow Cytometry) (Flow cytometric analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

LYVE1, Monoclonal Antibody (Cat# AAA30270)

Full Name
LYVE1 Antibody
Gene Names
LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
product-image-AAA23910_SDS_PAGE6.jpg SDS-PAGE (SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.)

CD34, Monoclonal Antibody (Cat# AAA23910)

Full Name
CD34 (Hematopoietic Stem Cell & Endothelial Marker)
Reactivity
Human, Rat. Others not known.
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
product-image-AAA16736_SC.jpg Standard Curve (Sample)

Cadherin 5, ELISA Kit (Cat# AAA16736)

Full Name
Canine Cadherin 5 ELISA Kit
Gene Names
CDH5; 7B4; CD144
Reactivity
Canine
Pricing
product-image-AAA11285_SC.jpg Standard Curve (Sample)

endothelium Selectin, E-Selectin, ELISA Kit (Cat# AAA11285)

Full Name
Human endothelium Selectin, E-Selectin ELISA Kit
Gene Names
SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
Reactivity
Human
Pricing
product-image-AAA19154_IHC7.jpg IHC (Immunohistochemistry) (Figure 7. IHC analysis of MYLK using anti-MYLK antibody (AAA19154).MYLK was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (AAA19154) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

MYLK, Polyclonal Antibody (Cat# AAA19154)

Full Name
Anti-MYLK Picoband antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
product-image-AAA10912_IF8.gif IF (Immunofluorescence) (Figure 10 Immunofluorescence Validation of TACE in Rat Brain (Pradillo et al, 2005)Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells.)

TACE, Polyclonal Antibody (Cat# AAA10912)

Full Name
TACE Antibody
Gene Names
ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
TACE Antibody is affinity chromatography purified via peptide column.
Pricing
product-image-AAA12247_FCM5.gif FCM (Flow Cytometry) (Staining of thrombin activated platelets with Mouse anti Human CD62P)

CD62P, Monoclonal Antibody (Cat# AAA12247)

Full Name
MOUSE ANTI HUMAN CD62P
Gene Names
SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Pricing
product-image-AAA13818_FCM6.jpg FCM (Flow Cytometry) (Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD31 Mouse Monoclonal Antibody (JC/70A) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13818)

Full Name
CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Cynomolgus Monkey, Rabbit.
Does not react with Rat and Pig
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
product-image-AAA12084_APP10.jpg Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

CD105, Monoclonal Antibody (Cat# AAA12084)

Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
product-image-AAA11933_APP10.jpg Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

CD105, Monoclonal Antibody (Cat# AAA11933)

Full Name
MOUSE ANTI HUMAN CD105
Gene Names
ENG; END; HHT1; ORW1
Reactivity
Cynomolgus monkey, Horse, Monkey, Primate, Rhesus Monkey
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
product-image-AAA12085_APP10.jpg Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

CD105, Monoclonal Antibody (Cat# AAA12085)

Full Name
MOUSE ANTI HUMAN CD105
Gene Names
ENG; END; HHT1; ORW1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
product-image-AAA12029_APP10.jpg Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

CD105, Monoclonal Antibody (Cat# AAA12029)

Full Name
MOUSE ANTI HUMAN CD105:RPE
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
product-image-AAA12083_APP10.jpg Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

CD105, Monoclonal Antibody (Cat# AAA12083)

Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
product-image-AAA11865_APP10.jpg Application Data (Published customer image: Mouse anti Human CD105 antibody, clone SN6 used for flow cytometry.Image caption:Phenotype change from HLA-A2+/CD11b+/CD105- to HLA-A2+/CD11b-/CD105+ on endothelium-adherent blood monocyte-derived cells with increase in size and granularity during co-culture. HLA-A2+ PBMCs (1x106 cells/well) were incubated for 2 h (Day 0) with HLA-A2- HUVECs, after which the non-adherent cells were removed by washing. The cell layers were analysed by three-colour flow cytometry staining for HLA-A2, CD11b and CD105 on (A) Day 1 and (B) Day 2. These plots were gated for HLA-A2+ cells. Forward scatter/side scatter dot plots gated for HLA-A2+ cells on Day 0 (C) and Day 2 (D) was shown. These are representative of 2 individual experiments.From: Tso C, Rye K-A, Barter P (2012) Phenotypic and Functional Changes in Blood Monocytes Following Adherence to Endothelium. PLoS ONE 7(5): e37091.)

CD105, Monoclonal Antibody (Cat# AAA11865)

Full Name
MOUSE ANTI HUMAN CD105:FITC
Gene Names
ENG; END; HHT1; ORW1
Applications
Flow Cytometry
Pricing
product-image-AAA12250_APP4.jpg Application Data (Published customer image: Mouse anti Pig IgM antibody, clone K52 1C3 used for thetection of IgM expressing cels in pig jejenum by immunofluorescence.Image caption:Colonisation expands IgM and IgA-producing mucosal plasma cells. (A) IgA staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to IgA (red) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the red channel (IgA); (iii) shows the original greyscale image for the blue channel (endothelium). Scale bar represents 10 um. (C) IgM staining in jejunal mucosa from a GF pig and (D) a colonised pig at 21 days of age. Sections were stained with antibodies to IgM (red). Scale bar represents 10 um. (E) Area of IgM and (F) IgA staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21days of age (see Table 1 for numbers of piglets in each experiment). * p)

IgM, Monoclonal Antibody (Cat# AAA12250)

Full Name
MOUSE ANTI PIG IgM
Reactivity
Pig
Applications
Flow Cytometry, Immunohistochemistry
Pricing

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