Search results for " DNA Synthesis"

4 results for " DNA Synthesis" - showing 1-4

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using CDKN1A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using CDKN1A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using CDKN1A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using CDKN1A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using CDKN1A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using CDKN1A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human breast cancer using CDKN1A/p21CIP1 Rabbit pAb (AAA10750) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CDKN1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody (AAA10750) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)

CDKN1A, Polyclonal Antibody (Cat# AAA10750)

Full Name

CDKN1A

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Reactivity

Human, mouse, rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

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Application Data

Application Data (Hela Cells were treated with BrdU for 3 hours and stained with Mouse anti BrdU antibody, clone Bu20a . Goat anti Mouse IgG (H/L) DyLight 549 conjugated antibody (red) was used as the secondary antibody. Cytoplasm was stained with Rabbit anti GAPDH antibody . As the secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody (green) was used. PureBlu DAPI (1351303) was used as nuclear counterstain (blue).)

Application Data (HeLa Cells were treated with 10 mug BrdU for 1 hour (B) or left untreated (A). Cells were stained with Mouse anti BrdU antibody, clone Bu20a at a dilution of 1/25. As a secondary antibody, Goat anti Mouse IgG (H/L) DyLight 549 conjugated antibody (red) was used at a 1/50 dilution. Cytoplasm was stained with Rabbit anti GAPDH antibody at a dilution of 1/100. As a secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody (green) was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.)

Application Data (Published Customer Image:Mouse Anti Bromodeoxyuridine antibody, clone Bu20a used to stain dividing crypt cells in rat small intestine by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:BrdU/ Bu20a/ Small intestine/ Rat.From: Furukawa S, Nagaike M, Ozaki K.Databases for technical aspects of immunohistochemistry.J Toxicol Pathol. 2017 Jan;30(1):79-107.)

Application Data (Published Customer image:Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.Image caption:Ghrelin does not significantly inhibit the rate of stem cell self-renewal in the SGZ of the DG of adult rats. Representative images identifying triple positive (BrdU+/Sox2+/S100B+) new adult-born astrocytes (arrows) and double-positive (BrdU+/Sox2+/S100B-) new adult-born stem cells (arrowheads). Statistical analysis was performed using one-way ANOVA with Bonferroni's post hoc test, n = 12 rats per group. Scale bar = 20mum.From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.Psychoneuroendocrinology. 2015 Jan;51:431-9.)

Application Data (Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.Image caption:Ghrelin enhances spatial pattern separation and adult hippocampal neurogenesis in adult rats.(D) Representative images of DCX+ immature neurones and new adult-born DG neurones (white arrows) co-expressing NeuN+ and BrdU+ (yellow). Scale bar = 200mum.From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.Psychoneuroendocrinology. 2015 Jan;51:431-9.)

Application Data (Mouse Anti Bromodeoxyuridine antibody, clone Bu20a used to label DNA synthesis in cultured mouse astrocytes by immunofluorescence.Image caption:Astrocyte response to injury involves proliferation which is attenuated by TTX and KB-R7943. (a) GFAP positive cultured rat cortical astrocytes (green) exhibit BrdU immunolabelling (red), seen in resting cells (top row) and scratched cells (second row). There is an increase in BrdU-positive cells along the edge of a scratch, which is attenuated with TTX and KB-R7943 treatment (third and fourth rows, respectively). Scale bar 200 mum. (b) After 24 h, there was a significant 77 +/- 9% increase in proliferation amongst cells along the edge of a wound (n=7) compared to cells in unscratched cultures (n=8), as measured by BrdU staining. Compared to untreated cells, TTX decreased proliferation by 54 +/- 4%, while KB-R7943 decreased proliferation by 46 +/- 9%. *p)

Application Data (Brdu-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution for 1 hour at room temperature. Goat anti Mouse IgG (H/L) DyLight 649 conjugated was used as the secondary detection reagent at a 1/50 dilution. PUREBLU Hoechst 33342 (1351304) was used to stain total DNA. B. BrdU-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution overnight at 4 degree C. Goat anti Mouse IgG (H/L) FITC conjugated antibody was used at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.)

BrdU, Monoclonal Antibody (Cat# AAA12259)

Full Name

Mouse Anti BrdU

Applications

Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Bamacan (Phospho-Ser1083) using nocodazole treated HeLa whole cell lysates.-/+ means absence or presence of N peptideï¼ˆnon-phospho peptide) and P peptide(phospho peptide).)

Bamacan, Polyclonal Antibody (Cat# AAA31455)

Full Name

Phospho-Bamacan (Ser1083) Antibody

Gene Names

SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

Standard Curve (Sample)

Antioncogene p21 protein, ELISA Kit (Cat# AAA16589)

Full Name

Human Antioncogene p21 protein ELISA Kit

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Reactivity

Human

Pricing

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4 results for " DNA Synthesis" - showing 1-4

CDKN1A, Polyclonal Antibody (Cat# AAA10750)

BrdU, Monoclonal Antibody (Cat# AAA12259)

Bamacan, Polyclonal Antibody (Cat# AAA31455)

Antioncogene p21 protein, ELISA Kit (Cat# AAA16589)

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4 results for " DNA Synthesis" - showing 1-4

CDKN1A, Polyclonal Antibody (Cat# AAA10750)

BrdU, Monoclonal Antibody (Cat# AAA12259)

Bamacan, Polyclonal Antibody (Cat# AAA31455)

Antioncogene p21 protein, ELISA Kit (Cat# AAA16589)

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