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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
34 results for "signal pathway" - showing 1-34
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
GAPDH, Polyclonal Antibody (Cat# AAA28693)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K9/K14/K18/K23/K27 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
Acetyl-Histone H3-K9/K14/K18/K23/K27, Polyclonal Antibody (Cat# AAA28357)
Full Name
Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SOCS1, Polyclonal Antibody (Cat# AAA28752)
Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.)
GBA/Glucosylceramidase, Monoclonal Antibody (Cat# AAA11697)
Full Name
Anti-GBA/Glucosylceramidase Rabbit Monoclonal Antibody
Gene Names
GBA; GCB; GBA1; GLUC
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using HSP27/HSP27/HSPB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
HSP27/HSPB1, Polyclonal Antibody (Cat# AAA28328)
Full Name
[KO Validated] HSP27/HSPB1 Rabbit pAb
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug AKT1 antibody . Western blot was performed from the immunoprecipitate using AKT1 antibody at a dilition of 1:1000.)
AKT1, Monoclonal Antibody (Cat# AAA28356)
Full Name
AKT1 Rabbit mAb
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Filamin A antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Filamin A, Polyclonal Antibody (Cat# AAA28331)
Full Name
Filamin A Rabbit pAb
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using WNT10B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WNT10B, Polyclonal Antibody (Cat# AAA28334)
Full Name
WNT10B Rabbit pAb
Gene Names
WNT10B; SHFM6; WNT-12
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using Bax Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.)
Bax, Polyclonal Antibody (Cat# AAA28318)
Full Name
Bax Rabbit pAb
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
SDS-PAGE
Thrombin/Antithrombin Complex (TAT), Recombinant Protein (Cat# AAA21174)
Full Name
Recombinant Thrombin/Antithrombin Complex (TAT)
Reactivity
Homo sapiens (Human)
Applications
WB
Purity
> 80%
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PDX1, Polyclonal Antibody (Cat# AAA31430)
Full Name
Phospho-PDX1 (Ser66) Antibody
Gene Names
PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%), Xenopus (100%)
Predicted Reactivity: Pig (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using YAP1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
YAP1, Polyclonal Antibody (Cat# AAA28345)
Full Name
YAP1 Rabbit pAb
Gene Names
YAP1; YAP; YKI; YAP2; YAP65
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat heart using IRS1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IRS1, Polyclonal Antibody (Cat# AAA28342)
Full Name
IRS1 Rabbit pAb
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Standard Curve (Sample)
Ghrelin (GHRL), ELISA Kit (Cat# AAA20373)
Full Name
Porcine Ghrelin (GHRL) ELISA Kit
Gene Names
GHRL; MTLRP
Reactivity
Porcine
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
Phospho-Histone H3-S10/T11, Polyclonal Antibody (Cat# AAA28391)
Full Name
Phospho-Histone H3-S10/T11 Rabbit pAb
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
Standard Curve (Sample)
Acetylcholinesterase (AchE), ELISA Kit (Cat# AAA20758)
Full Name
Human Acetylcholinesterase (AchE) ELISA Kit
Gene Names
ACHE; YT; ACEE; ARACHE; N-ACHE
Reactivity
Human
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K23 at dilution of 1:100. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.)
Acetyl-Histone H3-K23, Polyclonal Antibody (Cat# AAA28365)
Full Name
Acetyl-Histone H3-K23 Rabbit pAb
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
Standard Curve (Sample)
Inositol Triphosphate (IP3), ELISA Kit (Cat# AAA20058)
Full Name
Inositol Triphosphate (IP3) ELISA Kit
Reactivity
Most species
Standard Curve (Sample)
WNT1 Inducible Signaling Pathway Protein 1, ELISA Kit (Cat# AAA17254)
Full Name
Porcine WNT1 Inducible Signaling Pathway Protein 1 ELISA Kit
Gene Names
WISP1; CCN4; WISP1c; WISP1i; WISP1tc
Reactivity
Porcine
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of GALE using anti-GALE antibody (AAA19135).GALE was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALE Antibody (AAA19135) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GALE, Polyclonal Antibody (Cat# AAA19135)
Full Name
Anti-GALE Picoband antibody
Gene Names
GALE; SDR1E1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
Standard Curve (Sample)
Ghrelin (GHRL), ELISA Kit (Cat# AAA20779)
Full Name
Bovine Ghrelin (GHRL) ELISA Kit
Gene Names
GHRL; MTLRP
Reactivity
Bovine
Pricing
Standard Curve (Sample)
Glucose Transporter 1 (GLUT1), ELISA Kit (Cat# AAA20505)
Full Name
Human Glucose Transporter 1 (GLUT1) ELISA Kit
Gene Names
Glut1; 0074/12; CG1086; CG13908; CG43946; DmelCG43946; Dmglut1; DmGluT1; DmGLUT1; Glt1; GLU; glut1; l(3)61Ea; l(3)L1H; l(3)S007412
Reactivity
Human
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody . Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody at a dilition of 1:1000.)
NF-kB p65/RelA, Monoclonal Antibody (Cat# AAA28381)
Full Name
[KO Validated] NF-kB p65/RelA Rabbit mAb
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using [KO Validated] JAK1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
JAK1, Polyclonal Antibody (Cat# AAA28369)
Full Name
[KO Validated] JAK1 Rabbit pAb
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AAA28639) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)
SRC, Monoclonal Antibody (Cat# AAA28639)
Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Ran using anti-Ran antibody (AAA19131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse thymus tissue lysate,Lane 7: human A549 whole cell lysate,Lane 8: human 22RV1 whole cell lysate,Lane 9: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.)
Ran, Polyclonal Antibody (Cat# AAA19131)
Full Name
Anti-Ran Picoband Antibody
Gene Names
RAN; TC4; Gsp1; ARA24
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100ng/ml, 30 min), using Phospho-SMAD4 Antibody(upper) or Beta-actin (lower).)
Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA28715)
Full Name
Phospho-SMAD4 (T277) Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse
Predicted Species Reactivity: Rat
Predicted Species Reactivity: Rat
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target), Monoclonal Antibody (Cat# AAA23916)
Full Name
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target)
Gene Names
ICOSLG; B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml
Pricing
FCM (Flow Cytometry)
(WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(PCSK9 Antibody (N-term) (Cat. #AAA28774) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell(top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PCSK9, Polyclonal Antibody (Cat# AAA28774)
Full Name
PCSK9 Antibody (N-term)
Gene Names
PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
WB (Western Blot)
(Western blot analysis of Lipoprotein lipase expression in Human fetal liver lysate (AAA11694).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPL monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LPL)
Lipoprotein lipase, Monoclonal Antibody (Cat# AAA11694)
Full Name
Anti-Lipoprotein lipase Rabbit Monoclonal Antibody
Gene Names
LPL; LIPD; HDLCQ11
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-chromatography
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NRK Antibody (Center) (AAA28689). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
NRK, Polyclonal Antibody (Cat# AAA28689)
Full Name
NRK Antibody (Center)
Gene Names
NRK; NESK
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Raji cell lysates using SHP-1 mouse mAb.)
SHP-1, Monoclonal Antibody (Cat# AAA14102)
Full Name
Anti-SHP-1 Mouse mAb
Gene Names
PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity purified
Pricing
FCM (Flow Cytometry)
(SERPINH1 Antibody (Center) (Cat. #AAA28730) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SERPINH1, Polyclonal Antibody (Cat# AAA28730)
Full Name
SERPINH1 Antibody (Center)
Gene Names
SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing