Search results for "Tag mAb"

WB (Western Blot) (Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000Lane A: GST-mBID transfected E.coli cell lysate (0.2ug)Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug)Lane C: Empty vecter (0.2ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

WB (Western Blot) (Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000Lane A: GST-mBID transfected E.coli cell lysate (0.1ug)Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug)Lane C: Empty vecter (0.1ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

Application Data (Recognize GST tag fusion proteins which expressed by E. coli.Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250Lane A: GST-mBID transfected E.coli cell lysate (0.5ug)Lane B: GST-mBID transfected E.coli cell lysate (0.2ug)Lane C: GST-mBID transfected E.coli cell lysate (0.1ug)Lane D: GST-mBID transfected E.coli cell lysate (0.05ug)Lane E: GST-mBID transfected E.coli cell lysate (0.02ug)Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug)Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug)Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug)Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug)Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug)Lane K: Empty vecter (0.5ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

WB (Western Blot) (Recognize GST tag fusion proteins which expressed by 293 cell.Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500Lane A: GST-FABP4-DYKDDDDK transfected 293 Cell Lysate (30ug)Lane B: DYKDDDDK-FABP4-GST transfected 293 Cell Lysate (30ug)Lane C: DYKDDDDK-AGR1-GST transfected 293 Cell Lysate (30ug)Lane D: Negative controlSecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

WB (Western Blot) (Recognize GST tag fusion protein human USP7.Anti-GST Tag mouse monoclonal antibody at 1:4000Lane A: GST-USP7 (Recombinant protein) (100ng)Lane B: GST-USP7 (Recombinant protein) (50ng)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

Application Data (Recognize GST tag fusion protein human ERK2.Anti-GST Tag mouse monoclonal antibody at 1:4000Lane A: GST-ERK2 (Recombinant protein) (100ng)Lane B: GST-ERK2 (Recombinant protein) (50ng)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

IP (Immunoprecipitation) (Anti-GST Tag was immunoprecipitated using:Lane A: GST-ARG1-DYKDDDDK transfected 293 cell lysate (0.5mg)Lane B: DYKDDDDK-ARG1-GST transfected 293 cell lysate (0.5mg) 2 ug anti-HA Tag mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilutionSecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.)