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Application
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218 results for "Primary antibodies" - showing 1-50
WB (Western Blot)
(WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)
PSEN1, Polyclonal Antibody (Cat# AAA23585)
Full Name
PSEN1 antibody - middle region
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Affinity Purified
Pricing
IP (Immunoprecipitation)
(Figure 11 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10944)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(Figure 10 KD Validation in HeLa cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 ?M luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies (1139). DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10952)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Purity
DR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with CD99 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CD99, Monoclonal Antibody (Cat# AAA30274)
Full Name
CD99 Antibody
Gene Names
CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data
(Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)
CD172a, Monoclonal Antibody (Cat# AAA12056)
Full Name
MOUSE ANTI RAT CD172a:RPE
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12117)
Full Name
RAT ANTI MOUSE CD206:Biotin
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
IHC (Immunohistchemistry)
(Validation of PKR in Rat Lung Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (AAA10917) at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
PKR, Polyclonal Antibody (Cat# AAA10917)
Full Name
PKR Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PKR Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12122)
Full Name
RAT ANTI MOUSE CD206:FITC
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12125)
Full Name
RAT ANTI MOUSE CD206:RPE
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
Application Data
(Published customer image: Effects of EDTA versus media storage of patient blood samples on flow cytometric results. Peripheral blood cells were (a) stained immediate following collection with CD11b and CADO48A or maintained in EDTA collection tubes for (b) 24 or (c) 48 hours or in media for (d) 24 or (e) 48 hours prior to staining with CD11b and CADO48A. Both EDTA and media samples were kept refrigerated. These findings show a decrease over time of population distinction in EDTA with minimal changes when cells are kept in media up to 48 hours. All cells were gated on P1.From: Sherger et al. BMC Veterinary Research 2012 8:209)
CD11b, Monoclonal Antibody (Cat# AAA12092)
Full Name
MOUSE ANTI DOG CD11b
Gene Names
ITGAM; CD11B
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Pricing
ICC (Immunocytochemistry)
(Figure 10 Immunocytochemistry Validation of PD-L1Immunocytochemical analysis of 4% paraformaldehyde-fixed PD-L1 transfected 293 cells labeling PD-L1 with at 1 μg/ml, followed by Goat anti-mouse IgG secondary antibody at 1/250 dilution (red). Lower left: Use mouse IgG antibody at 1 μg/ml as control.)
PDL1, Monoclonal Antibody (Cat# AAA10992)
Full Name
PDL1 Antibody [6H10]
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A purified
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12120)
Full Name
RAT ANTI MOUSE CD206:FITC
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of TMEM41B in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
TMEM41B, Polyclonal Antibody (Cat# AAA11035)
Full Name
TMEM41B (CT) Antibody
Reactivity
Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
Applications
Immunofluorescence, Western Blot
Purity
TMEM41B Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(Detection of Mouse anti Bovine CD86 in a flow cytometric analysis of bovine peripheral blood lymphocytes using Goat anti Mouse IgG:FITC (AAA12304))
IgG, Polyclonal Antibody (Cat# AAA12304)
Full Name
GOAT ANTI MOUSE IgG:FITC (RAT ADSORBED)
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(CDK4 Antibody (C-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CDK4, Polyclonal Antibody (Cat# AAA28747)
Full Name
CDK4 Antibody (C-term)
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12182)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)
S100B, Polyclonal Antibody (Cat# AAA28759)
Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat (Predicted: Rabbit)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Orexin receptor 1, Polyclonal Antibody (Cat# AAA13669)
Full Name
Goat anti-Orexin receptor 1 Antibody
Gene Names
HCRTR1; OX1R
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Sheep
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(PLD6 Antibody (Center) (Cat# AAA28684) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).)
PLD6, Polyclonal Antibody (Cat# AAA28684)
Full Name
PLD6 Antibody (Center)
Gene Names
PLD6; ZUC
Reactivity
Human, Mouse
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistchemistry)
(Figure 5 Immunohistochemistry Validation of TIM-1Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
TIM-1, Polyclonal Antibody (Cat# AAA10935)
Full Name
TIM-1 Antibody
Gene Names
HAVCR1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
TIM-1 Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
CD38, Polyclonal Antibody (Cat# AAA28702)
Full Name
CD38 Antibody (C-term)
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Figure 9 KD Validation of PUMA (Han et al., 2010)Immunoblot analyses of Tet-induced p53 cells treated with NOXA, Puma, Bim or non-targeting siRNAs that were utilized in this experiment. PUMA protein levels were markedly reduced in PUMA KD cells detected by anti-PUMA antibodies (3041).)
PUMA, Polyclonal Antibody (Cat# AAA10943)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating GAPDH in Hela whole cell lysateLane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (5ul) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug)For western blotting, the blot was detected at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000)
GAPDH, Monoclonal Antibody (Cat# AAA27042)
Full Name
GAPDH Monoclonal Antibody
Reactivity
Human, Mouse, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of TMEM41B in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
TMEM41B, Polyclonal Antibody (Cat# AAA11036)
Full Name
TMEM41B (NT) Antibody
Reactivity
Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Horse (100%); Chimpanzee (100%).
Predicted species reactivity based on immunogen sequence: Horse (100%); Chimpanzee (100%).
Applications
Immunofluorescence, Western Blot
Purity
TMEM41B Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
GAPDH, Polyclonal Antibody (Cat# AAA28776)
Full Name
GAPDH Antibody (N-term)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Overlay Peak curve showing 293F cells transfected with GST stained with AAA28065 (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
GST, Monoclonal Antibody (Cat# AAA28065)
Full Name
GST Monoclonal Antibody
Reactivity
All
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat Testis labeling TMPRSS2 with 9569 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
TMPRSS2, Polyclonal Antibody (Cat# AAA11037)
Full Name
TMPRSS2 (CT) Antibody
Gene Names
TMPRSS2; PP9284; PRSS10
Reactivity
Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Monkey (100%); Gorilla (100%); Cat (92%).
Predicted species reactivity based on immunogen sequence: Monkey (100%); Gorilla (100%); Cat (92%).
Applications
Immunofluorescence, Western Blot
Purity
TMPRSS2 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(Published customer imageSLAMF3 blockade in human hepatocytes is associated with lower susceptibility to HCV. (A) SLAMF3 was stained in primary human hepatocytes (PHHs) and cells from the Huh-7 human hepatoma cell line with a specific antibody (HLy9.1.25 clone; grey) and an isotype-matched control (empty). One of four independent experiments is shown. Huh-7 cells were transfected with scrambled control (sc) siRNA or three specific siRNAs (#1, #2 and #3) targeting SLAMF3, prior to infection with HCVcc; siRNA efficiency was checked by quantifying SLAMF3 mRNA (B) and the CD81 expression level (C) by flow cytometry analysis at 48 h post-transfection. Results are presented as the mean +/-SD (n = 3). Intracellular viral RNA was quantified at 72 h p.i. (D) and the infection was measured at 72 h p.i. by focus-forming units FFUs counting (E) (as inhibition percent; mean of three independent experiments; error bars: SD. **p)
CD229, Monoclonal Antibody (Cat# AAA11951)
Full Name
MOUSE ANTI HUMAN CD229
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Applications
Flow Cytometry, Immunoprecipitation
Pricing
Application Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta5 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power))
ESTROGEN RECEPTOR BETA 5, Monoclonal Antibody (Cat# AAA12216)
Full Name
MOUSE ANTI HUMAN ESTROGEN RECEPTOR BETA 5
Gene Names
ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
Applications
Immunohistochemistry, Western Blot
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12183)
Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of AAA27043 in Hela whole cell lysate. Lane 2: AAA27043 (5ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug) For western blotting, the blot was detected with AAA27043 at 1:5000, and a HRPconjugated Protein G antibody was used as the secondary antibody at 1:2000)
GAPDH, Monoclonal Antibody (Cat# AAA27043)
Full Name
GAPDH Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.)
HDAC2, Polyclonal Antibody (Cat# AAA28801)
Full Name
HDAC2 Antibody (C-term)
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IP (Immunoprecipitation)
(Figure 13 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10930)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(Figure 9 Immunohistochemistry Validation of hRIP3 in Human Colon Tissue Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-hRIP3 antibody (8963) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
hRIP3, Polyclonal Antibody (Cat# AAA11031)
Full Name
hRIP3 Antibody
Gene Names
RIPK3; RIP3
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
hRIP3 Antibody is Protein A purified.
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12118)
Full Name
RAT ANTI MOUSE CD206:Biotin
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with XRCC6 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with XRCC6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). XRCC6 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)
XRCC6, Polyclonal Antibody (Cat# AAA28768)
Full Name
XRCC6 Antibody (C-term)
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA28066 (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ACTB, Monoclonal Antibody (Cat# AAA28066)
Full Name
ACTB Monoclonal Antibody
Reactivity
Human, Mouse, Rat, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
WB (Western Blot)
(Figure 1, Wstern blot: primary antibody, anti-S1(MERS-CoV) rabbit polyclonal antibody (1:2000 dilution); secondary antibody, HRPconjugated goat anti-rabbit IgGA, full length S1 protein of MERS-CoVB, receptor binding domain of S1 (MERS-CoV) protein)
S1 (MERS-CoV), Antibody (Cat# AAA13694)
Full Name
Anti-S1 (MERS-CoV) Rabbit Polyclonal Antibody
Applications
Western Blot
Purity
Protein A sepharose purified IgG
Pricing
FCM (Flow Cytometry)
(SEPT9 Antibody (A555) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SEPT9, Polyclonal Antibody (Cat# AAA28793)
Full Name
SEPT9 Antibody (C-term)
Gene Names
SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12124)
Full Name
RAT ANTI MOUSE CD206:RPE
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
WB (Western Blot)
(TFAM Antibody (C-term) western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the TFAM antibody detected the TFAM protein (arrow).)
TFAM, Polyclonal Antibody (Cat# AAA28696)
Full Name
TFAM Antibody (C-term)
Gene Names
TFAM; TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.)
Bid, Polyclonal Antibody (Cat# AAA28769)
Full Name
Bid Antibody (BH3 Domain Specific)
Gene Names
BID; FP497
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot of Latexin (arrow) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2))
Latexin / MUM, Polyclonal Antibody (Cat# AAA12323)
Full Name
Rabbit Polyclonal to Human Latexin / MUM
Gene Names
LXN; ECI; TCI
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Immunoaffinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of LIN28B Antibody (N-term) in HL60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified Pab.)
LIN28B, Polyclonal Antibody (Cat# AAA12320)
Full Name
Rabbit Polyclonal to Human LIN28B
Gene Names
LIN28B; CSDD2
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Ammonium sulfate precipitation
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.)
BHLH3, Polyclonal Antibody (Cat# AAA28734)
Full Name
BHLH3 Antibody (N-term)
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of (AAA28653) on paraffin-embedded Human breastcarcinoma tissue. Tissue was fixed withformaldehyde at room temperature. Heatinduced epitope retrieval was performed byEDTA buffer (pH9. 0). Samples wereincubated with primary antibody(1:100) for 1hour at room temperature. Undiluted CRFAnti-Polyvalent HRP Polymer antibody wasused as the secondary antibody.)
Phospho-CDC25A (S124), Polyclonal Antibody (Cat# AAA28653)
Full Name
Phospho-CDC25A (S124) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human (Predicted Reactivity: Rat)
Applications
WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
TMPRSS2, Polyclonal Antibody (Cat# AAA11038)
Full Name
TMPRSS2 (IN) Antibody
Gene Names
TMPRSS2; PP9284; PRSS10
Reactivity
Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Horse (100%); Rabbit (100%); Monkey (100%); Sheep (100%); Gorilla (100%); Cat (100%).
Predicted species reactivity based on immunogen sequence: Horse (100%); Rabbit (100%); Monkey (100%); Sheep (100%); Gorilla (100%); Cat (100%).
Applications
Immunofluorescence, Western Blot
Purity
TMPRSS2 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12186)
Full Name
RAT ANTI MOUSE CD11b:RPE
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Parkin, Polyclonal Antibody (Cat# AAA28668)
Full Name
Parkin Antibody (C-term)
Gene Names
PARK2; PDJ; PRKN; AR-JP; LPRS2
Reactivity
Human, mouse
Applications
WB, EIA, IF, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
PACSIN2, Polyclonal Antibody (Cat# AAA28758)
Full Name
PACSIN2 Antibody (C-term)
Gene Names
PACSIN2; SDPII
Reactivity
Human, mouse
Applications
Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing