Search results for "Inflammatory Marker"

5 results for "Inflammatory Marker" - showing 1-5

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . High power)

Application Data (Published customer image: Massive activation and productive infection of microglia in CD4-depleted RMs. (a) ISH showing the levels of SIV vRNA+ cells in brain from a representative SIV-uninfected control (top), SIV-infected control (middle) and CD4-depleted SIV-infected (bottom) animal. The amount of SIV vRNA+ cells was markedly higher in CD4-depleted animals than in controls. (b) Immunofluorescence staining for CD163 (left panels), HLA-DR (middle panels) and proliferating cell nuclear antigen (PCNA; right panels) in the parenchyma of one representative SIV-uninfected control (top), one SIV-infected control (middle) and one SIV-infected CD4-depleted (bottom) RM. Nuclei are stained in green; markers of interest in red. (c) Quantitative analyses showing the number of cells per mm2 of tissue that stained positively for the markers of interest. Increased expression of CD163, HLA-DR and PCNA was found in CD4-depleted animals (orange square; n = 4) when compared to both groups of controls (SIV- open circle, n = 3; SIV+ closed circle, n = 3). (d) Single and combined staining for IBA-1 (green), CD163 (blue), and SIV-vRNA (red) in the brain of one representative SIV-infected CD4 depleted RM. The box on the right is a magnification of a microglial cell (IBA-1+) that expresses CD163 and is productively infected (SIV vRNA+).From: Micci L, Alvarez X, Iriele RI, Ortiz AM, Ryan ES, et al. (2014) CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells. PLoS Pathog 10(10): e1004467.)

Application Data (Published customer image: Massive SIV infection of LN and intestinal macrophages in CD4-depleted RMs. (a) Immunofluorescence staining for T cell (CD3; blue) and macrophage (CD68 and/or CD163; green) lineage markers combined with in situ hybridization for SIV vRNA (red) in the LN isolated at day 42 post-infection from one representative undepleted control (left) and one CD4-depleted RM. The vast majority of SIV vRNA+ cells express CD3 in undepleted control but express CD68/CD163 in CD4-depleted RM. (b) The same staining is shown for colon (top) and jejunum (bottom) tissues in two representative CD4-depleted animals. (c) Quantitative image analysis of LN and mucosal tissues showing the fraction of SIV vRNA+ cells that express CD3 or CD68/CD163 in CD4-depleted (n = 12; the 8 animals in this study plus the 4 in Ortiz et al 2011) or undepleted control RMs (n = 6; two SIVmac251 infected animals belonging to a different study were added as controls). (d) Relative abundance of SIV vRNA+ in productively infected T cells and macrophages, determined by measuring the volumetric sum of the SIV vRNA+ intensity integration values in situ from confocal images collected under identical laser settings, is shown in four CD4-depleted RMs. Macrophages on average have higher per cell SIV vRNA+ content compared to CD4+ T cells within the same host. Statistical analyses were determined by Mann-Whitney test.From: Micci L, Alvarez X, Iriele RI, Ortiz AM, Ryan ES, et al. (2014) CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells. PLoS Pathog 10(10): e1004467.)

Application Data (Published customer image:Immunofluorescence analysis of myocardial HO-1 and CD163 expression. Surgical non-treated (NT) control animals showed minimal HO-1 (green) expression in resident CD163+ perivascular macrophages (red) (A -C). Sporadic HO-1 expression (white arrow) localized to CD163+ cells observed with Hb alone (D -F). With LPS alone, CD163+ infiltrates and perivascular macrophages showed minimal HO-1 expression (white arrowhead) (G -I). Increased HO-1 expression localized to CD163+ infiltrates and perivascular macrophages observed with LPS + Hb (white arrows) (J -L,M -O). Hp reduces HO-1 expression in CD163+ infiltrates and perivascular cells (P -R,S -U). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar = 10 um.From: Baek JH, Zhang X, Williams MC, Schaer DJ, Buehler PW, D'Agnillo F. Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin. Toxins (Basel). 2014 Mar 31;6(4):1244-59.)

Application Data (Published customer image: Rasmussen's encephalitis. (A) Areas of active encephalitis and cortical scarring highlighted with increased numbers of HLADR-positive microglia/macrophages, as well as interstitial rod-like CD163-positive microglia cells (inset). (B) On adjacent sections, labelling with pS6 235/236 showed a similar distribution of cellular labelling as well as with pS6 240/244 (C). (D) pS6 240/244 highlighted many of the enlarged dysmorphic neurones in RE cases, as confirmed with co-labelling with neurofilaments (inset). (E) pS6 236/26 demonstrated labelling of smaller glial cells as well as (F) bipolar rod shape cells. Double labelling studies in RE cases confirmed the majority of pS6 labelled cells were not GFAP-positive astroglia (G) but co-labelled with populations of iba1-positive cells (microglial marker) (H), nestin and doublecortin (inset) (I) positive cells. Bar in A, B, C equivalent to approximately 100 microns, in d approximately 50 microns and E, F, G, H, I approximately 30 microns.From: Liu J, Reeves C, Michalak Z, Coppola A, Diehl B, Sisodiya SM, Thom M. Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies. Acta Neuropathol Commun. 2014 Jul 8;2:71.)

Application Data (Published customer image: Tumor-promoting phenotype of the myeloid clusters. IHC staining demonstrating the expression of CD163, IL-6, IL-10, VEGF-A, MMP-9, SDF-1 and Bcl-xL by the myeloid cells associated with anthracosis. For each protein, representative images showing positive and negative staining areas were selected from the same slide. The quantification was performed by analyzing random images of myeloid cluster areas associated with anthracosis and other areas (10 images for each group) from 10 patients. Two-tailed Student's t-test was used for statistical analysis. Shown are means +/- SEM, *** P)

Application Data (Published customer image: Morphologic and phenotypic characterization of porcine alveolar macrophage cells (PAM) by flow cytometry using SWC3, SWC1, CD163, CD14, CD16, MHCII and DC-sign staining. This figure is a representative of three independent experiments.From: Seeboth J, Solinhac R, Oswald IP, Guzylack-Piriou L. The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig. Vet Res. 2012 Apr 24;43:35..)

Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

CD163, Monoclonal Antibody (Cat# AAA12100)

Full Name

MOUSE ANTI HUMAN CD163

Gene Names

CD163; M130; MM130

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Immunohistochemistry, Western Blot

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Application Data (Published customer image: Cytokine expression in segregated populations of cells following stroke. (A, B) Dot plots showing CD11b+CD45high macrophages/granulocytes (upper right quadrants) and CD11b+CD45dim microglia (bottom right quadrants) expressing IL-1beta (A) or TNF-a (B). (C-J) Bar graphs showing numbers and proportions of IL-1beta (C, D), TNF-a (F, G) and IL-1beta/TNF-a co-expressing (I, J) CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in unmanipulated control mice (n = 10), in mice 6 (n = 7), 12 (n = 7), or 24 hours after pMCAO (n = 10), and in sham-operated mice 24 hours after pMCAO (n = 7). (E, H) Comparison of the MFI values for IL-1beta (E) and TNF-a (H) in viable CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in unmanipulated mice, in mice 6, 12, or 24 hours after pMCAO, and in sham-operated mice 24 hours after pMCAO. Macrophages/granulocytes express significantly more IL-1beta than do microglial in unmanipulated mice, in mice 6, 12, or 24 hours after pMCAO, and in sham-operated mice 24 hours after pMCAO (E), whereas microglial cells express significantly higher levels of TNF-a than do macrophages/granulocytes at 12 h and 24 hours, and in sham-operated mice 24 hours after pMCAO (H). (K) CD11b+CD45highGr1- macrophages and not CD11b+CD45highGr1+ granulocytes are the main producers of IL-1beta and TNF-a 24 hours after pMCAO. *P < 0.05, **P < 0.01, and ***P < 0.001.From: http://www.jneuroinflammation.com/content/5/1/46.)

Application Data (Published customer image:e) Cryosectioned brain samples from day 6 p.i. were stained for WNV envelope protein (green), CD45 (as a pan-leukocyte marker; red), and DAPI (blue) and utilized for laser scanning confocal microscopy (20X images are shown and are representative of n = 5 mice per group).From: Wang P, Bai F, Zenewicz LA, Dai J, Gate D, et al. (2012) IL-22 Signaling Contributes to West Nile Encephalitis Pathogenesis. PLoS ONE 7(8): e44153.)

Application Data (Published customer image: Colocalization of activated microglial cells with Abeta plaques and IL-1beta immunoreactivity in the TgAPPsw mice with reduced GRK5. Panels A-C, IF staining of Abeta+ plaques (A, red) and surrounding CD45+ microglial cells (B, green), as well as their merged view (C) in the double mice. Scale bar: 50 um for panels A-C. Panels D-F, IF staining of Abeta+ plaques (D, red) and surrounding CD11b+ microglial cells (E, green), as well as their merged view (F) in the double mice. Scale bar: 50 um for panels D-F. Panel G, an example of merged view for CD45 (green) and CD11b/c (clone OX42, red) co-staining of the microglial cells. The image showed that, at least in this particular experimental paradigm, the CD45 antibody stained more specifically for the microglial cell profiles; while the OX42 antibody, in addition to its positive staining of the microglial cells, also non-specifically decorated the plaques. Scale bar: 45 um. Panel H, an example of merged view for Abeta+ plaques (red) and surrounding IL-1beta immunoreactivity (green) in the double mice. Scale bar: 30 um. Panel I, colocalization of CD45+ microglial cells (green) with IL-1beta immunoreactivity (red) in the double mice. Scale bar: 30 um. Blue indicates reference DAPI staining of nuclei.From: Li et al. Journal of Neuroinflammation 2008 5:24.)

Application Data (Published customer image: Inflammatory response following permanent MCA occlusion. (A-C) Dot plots of viable CD11b+CD45high macrophages/granulocytes (top right quadrants) and CD11b+CD45dim microglia (bottom right quadrants) in cortex from unmanipulated control mice (A, B), and mice exposed to pMCAO with 24 hour survival (C). (D) At 24 hours, flow cytometric analysis of the CD11b+CD45high profiles showed that approximately half of the population consisted of CD45highGr1+ granulocytes. (E) Quantification of CD11b+CD45dim and CD11b+CD45high cells in unmanipulated control mice (n = 10), in mice 6 (n = 7), 12 (n = 7), or 24 hours after pMCAO (n = 10), and in sham-operated mice 24 hours after pMCAO (n = 7). (F) Bar graphs showing equal recruitment of CD11b+CD45highGr1- macrophages and CD11b-CD45highGr1+ granulocytes in unmanipulated mice, in mice 6, 12, or 24 hours after pMCAO, and in sham-operated mice 24 hours after pMCAO. (G, H) Bar graphs showing the mean fluorescent intensity (MFI) of CD45 expression by CD45dim microglia (G) and CD45high macrophages/granulocytes (H). *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen et al. Journal of Neuroinflammation 2008 5:46.)

Application Data (Published customer image Percentage of microgliosis (mean +/- s.e.m) by area in (a) Tg APPsw/CD40 def. mice versus Tg APPsw mice and in (b) Tg PSAPP/CD40 def. mice versus Tg PSAPP mice at 22 to 24 months of age calculated by quantitative image analysis. Post hoc comparison between groups are indicated by the marked bars (* p < 0.05; ** p < 0.01). Representative photographs of brain area in (c) Tg APPsw, (e) Tg APPsw/CD40 def., (d) Tg PSAPP and (f) Tg PSAPP/CD40 def. mice stained with CD45 antibody (each bar represents 0.1 mm).Laporte et al..)

Application Data (Published customer image: Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen et al. Journal of Neuroinflammation 2008 5:46.)

Application Data (Published customer image: Increased CD45+ microglial cells in hippocampus and cortex of the TgAPPsw mice with reduced GRK5. Representative IF results with anti-CD45 staining (green) in hippocampus (A-D) and cortex (F-I) of WT (A &F), heterozygote GRK5KO (B &G), TgAPPsw (C &H), and the double mice (D &I), respectively. Scale bar in panel I is for panels A-D and F-I: 100 um. Panels E &J, examples of high magnification views of CD45+-microglial cells in hippocampus (E) and cortex (J) of the double mice that show details of activated microglial morphology. Scale bar in panel J is for panels E &J: 20 um. Blue indicates reference DAPI staining of nuclei.From: Li et al. Journal of Neuroinflammation 2008 5:24.)

Application Data (Published customer image: Sensitivity of cytokine detection using flow cytometry. Histograms and dot plots of IL-1beta (A) and TNF-a (B) expression in LPS-activated peritoneal macrophages versus macrophages/granulocytes isolated from cortex 24 hours after pMCAO. Light colored histograms represent cells stained with isotype control antibodies and filled histograms represent cells stained with antibodies for either IL-1beta (A) or TNF-a (B).From: Clausen et al. Journal of Neuroinflammation 2008 5:46)

Application Data (Published customer image: Chronic effects of celastrol on microgliosis in Tg PS1/APPsw mice. A) Representative photomicrographs (taken with a 20x and 60x objective providing a respective magnification of 200x and 600x respectively) depicting the presence of CD45 reactive microglia around Abeta deposits in Tg PS1/APPsw treated with a placebo and celastrol. B) Histogram representing the burden of activated microglia (CD45 positive) in the cortex of Tg PS1/APPsw mice treated with placebo and celastrol pellets. Statistically significant difference in microgliosis burden (P < 0.03) was observed between placebo and celastrol treated mice. (* P < 0.05)..)

CD45, Monoclonal Antibody (Cat# AAA11896)

Full Name

RAT ANTI MOUSE CD45

Gene Names

Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation

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S100A9, Monoclonal Antibody (Cat# AAA13837)

Full Name

S100A9 (Macrophage Marker) Mouse Monoclonal Antibody

Gene Names

S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387

Reactivity

Human and Rat. Others not tested.

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . High power)

Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1, red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1, red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

CD163, Monoclonal Antibody (Cat# AAA11943)

Full Name

MOUSE ANTI HUMAN CD163

Gene Names

CD163; M130; MM130

Reactivity

Guinea Pig, Pig, Rhesus Monkey, Sheep

Applications

Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Western Blot

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IF (Immunofluorescence) (staining A549 cells cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

Application Data (E4BP4 mediates glucocorticoid-regulated adipogenesis through COX2 YangYang a, b , Hongkui Wei a, b , Tongxing Song a, b , Anle Cai a, b , Yuanfei Zhou a, b , Jie Peng a, b , Siwen Jiang b, c , Jian Peng a, b, *)

WB (Western Blot) (Western blot analysis of extracts from Mouse spleen, using Cox2 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 70 kDa.)

WB (Western Blot) (Western blot analysis of Cox2 expression in A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (At 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IF (Immunofluorescence) (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)

IF (Immunofluorescence) (At 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibod)

Cox2, Polyclonal Antibody (Cat# AAA31143)

Full Name

Cox2 Antibody

Gene Names

PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

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5 results for "Inflammatory Marker" - showing 1-5

CD163, Monoclonal Antibody (Cat# AAA12100)

CD45, Monoclonal Antibody (Cat# AAA11896)

S100A9, Monoclonal Antibody (Cat# AAA13837)

CD163, Monoclonal Antibody (Cat# AAA11943)

Cox2, Polyclonal Antibody (Cat# AAA31143)

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5 results for "Inflammatory Marker" - showing 1-5

CD163, Monoclonal Antibody (Cat# AAA12100)

CD45, Monoclonal Antibody (Cat# AAA11896)

S100A9, Monoclonal Antibody (Cat# AAA13837)

CD163, Monoclonal Antibody (Cat# AAA11943)

Cox2, Polyclonal Antibody (Cat# AAA31143)

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