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Application

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46 results for "Goat Anti Rat IgG chain specific " - showing 1-46


product-image-AAA14908_APP.jpg Application Data (FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-PE)

Goat Anti-Rat IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14908)

Full Name
Goat Anti-Rat IgG-HRP
Reactivity
Rat IgM; may react with immunoglobulins from other species.
Applications
ELISA
Purity
Affinity chromatography on pooled rat IgG covalently linked to agarose
Pricing
product-image-AAA29919_FCM7.jpg FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Alpha-tubulin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

Alpha-Tubulin, Polyclonal Antibody (Cat# AAA29919)

Full Name
Alpha-Tubulin Antibody
Gene Names
TUBA4A; TUBA1; H2-ALPHA
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Immunofluorescence
Purity
Peptide affinity purified
Pricing
product-image-AAA14899_APP.jpg Application Data (BALB/c mouse splenocytes were stained with Goat F(ab)2 Anti-Mouse Kappa-BIOT and Rat Anti-Mouse CD19-PE followed by Streptavidin-FITC.)

Goat F(ab')2 Anti-Mouse kappa (kappa chain specific), Polyclonal Secondary Antibody (Cat# AAA14899)

Full Name
Goat F(ab')2 Anti-Mouse Kappa-LE/AF
Reactivity
Mouse
Applications
Flow Cytometry
Purity
Gel filtration chromatography of pepsin digested antibody
Pricing
product-image-AAA31146_WB7.jpg WB (Western Blot) (Western blot analysis of Tubulin alpha expression in various sample)

Tubulin alpha, Polyclonal Antibody (Cat# AAA31146)

Full Name
Tubulin alpha Antibody
Gene Names
TUBA1B; K-ALPHA-1
Reactivity
Human, Mouse, Rat, Pig, Bovine, Rabbit, Chicken, Fish
Predicted: Horse, Sheep, Dog
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA19262_FCM8.jpg FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of C6 cells using anti-non-muscle Myosin IIB/MYH10 antibody (AAA19262).Overlay histogram showing C6 cells stained with AAA19262 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-non-muscle Myosin IIB/MYH10 Antibody (AAA19262,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

non-muscle Myosin IIB/MYH10, Polyclonal Antibody (Cat# AAA19262)

Full Name
Anti-non-muscle Myosin IIB/MYH10 Antibody
Gene Names
MYH10; NMMHCB; NMMHC-IIB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA12182_APP9.jpg Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

CD11b, Monoclonal Antibody (Cat# AAA12182)

Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
product-image-AAA12184_APP9.jpg Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

CD11b, Monoclonal Antibody (Cat# AAA12184)

Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
product-image-AAA31078_IHC6.jpg IHC (Immunohistchemistry) (Annexin II Antibody for IHC in human colon tissue)

Annexin II, Polyclonal Antibody (Cat# AAA31078)

Full Name
Annexin II Antibody
Gene Names
ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA19239_FCM6.jpg FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of SiHa cells using anti-Glutaminase/GLS antibody (AAA19239).Overlay histogram showing SiHa cells stained with AAA19239 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutaminase/GLS Antibody (AAA19239,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Glutaminase/GLS, Polyclonal Antibody (Cat# AAA19239)

Full Name
Anti-Glutaminase/GLS Antibody
Gene Names
GLS; GAC; GAM; KGA; GLS1; AAD20
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA12183_APP9.jpg Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

CD11b, Monoclonal Antibody (Cat# AAA12183)

Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
product-image-AAA12185_APP9.jpg Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

CD11b, Monoclonal Antibody (Cat# AAA12185)

Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
product-image-AAA11965_APP10.jpg Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

CD25, Monoclonal Antibody (Cat# AAA11965)

Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
product-image-AAA11966_APP10.jpg Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

CD25, Monoclonal Antibody (Cat# AAA11966)

Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
Pricing
product-image-AAA11668_ICC7.jpg ICC (Immunocytochemistry) (Figure 7. IHC analysis of ERAB using anti-ERAB antibody (AAA11668).ERAB was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERAB Antibody (AAA11668) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ERAB, Polyclonal Antibody (Cat# AAA11668)

Full Name
Anti-ERAB Antibody
Gene Names
HSD17B10; ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; 17b-HSD10; DUPXp11.22
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
product-image-AAA12044_APP10.jpg Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

CD25, Monoclonal Antibody (Cat# AAA12044)

Full Name
MOUSE ANTI RAT CD25:RPE
Gene Names
Il2ra; IL2RAC
Applications
Flow Cytometry
Pricing
product-image-AAA12186_APP9.jpg Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

CD11b, Monoclonal Antibody (Cat# AAA12186)

Full Name
RAT ANTI MOUSE CD11b:RPE
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
product-image-AAA12231_APP9.jpg Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

CD11b, Monoclonal Antibody (Cat# AAA12231)

Full Name
RAT ANTI MOUSE CD11b:Low Endotoxin
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
Pricing
product-image-AAA12181_APP9.jpg Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

CD11b, Monoclonal Antibody (Cat# AAA12181)

Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
product-image-AAA19175_IHC6.jpg IHC (Immunohistchemistry) (Figure 6. IHC analysis of DYNLT1 using anti-DYNLT1 antibody (AAA19175).DYNLT1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DYNLT1 Antibody (AAA19175) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

DYNLT1, Polyclonal Antibody (Cat# AAA19175)

Full Name
Anti-DYNLT1 Picoband antibody
Gene Names
DYNLT1; CW-1; TCTEL1; tctex-1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
product-image-AAA19270_FCM8.png FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of C6 cells using anti-Clathrin heavy chain/CLTC antibody (AAA19270).Overlay histogram showing C6 cells stained with AAA19270 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Clathrin heavy chain/CLTC Antibody (AAA19270,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Clathrin heavy chain/CLTC, Polyclonal Antibody (Cat# AAA19270)

Full Name
Anti-Clathrin heavy chain/CLTC Antibody
Gene Names
CLTC; Hc; CHC; CHC17; CLH-17; CLTCL2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19156_IHC6.jpg IHC (Immunohistchemistry) (Figure 6. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).Integrin alpha 5 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 5 Antibody (AAA19156) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Integrin alpha 5, Polyclonal Antibody (Cat# AAA19156)

Full Name
Anti-Integrin alpha 5 Picoband antibody
Gene Names
ITGA5; FNRA; CD49e; VLA-5; VLA5A
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
product-image-AAA19154_IHC7.jpg IHC (Immunohistochemistry) (Figure 7. IHC analysis of MYLK using anti-MYLK antibody (AAA19154).MYLK was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (AAA19154) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

MYLK, Polyclonal Antibody (Cat# AAA19154)

Full Name
Anti-MYLK Picoband antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
product-image-AAA19277_FCM13.png FCM (Flow Cytometry) (Figure 13. Flow Cytometry analysis of THP-1 cells using anti-Alpha B Crystallin/CRYAB antibody (AAA19277).Overlay histogram showing THP-1 cells stained with AAA19277 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha B Crystallin/CRYAB Antibody (AAA19277, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Alpha B Crystallin/CRYAB, Polyclonal Antibody (Cat# AAA19277)

Full Name
Anti-Alpha B Crystallin/CRYAB Antibody
Gene Names
CRYAB; CRYA2; CTPP2; HSPB5; CMD1II
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19274_FCM8.jpg FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U87 cells using anti-PSMB5/MB1 antibody (AAA19274).Overlay histogram showing U87 cells stained with AAA19274 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMB5/MB1 Antibody (AAA19274, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PSMB5/MB1, Polyclonal Antibody (Cat# AAA19274)

Full Name
Anti-PSMB5/MB1 Antibody
Gene Names
PSMB5; X; MB1; LMPX
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19383_IF8.jpg IF (Immunofluorescence) (Figure 8. IF analysis of eRF1/ETF1 using anti- eRF1/ETF1 antibody (AAA19383).eRF1/ETF1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- eRF1/ETF1 Antibody (AAA19383) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

eRF1/ETF1, Monoclonal Antibody (Cat# AAA19383)

Full Name
Anti-eRF1/ETF1 Antibody (monoclonal, 3E5)
Gene Names
ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19162_IHC6.jpg IHC (Immunohistchemistry) (Figure 6. IHC analysis of S100A10 using anti-S100A10 antibody (AAA19162).S100A10 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-S100A10 Antibody (AAA19162) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

S100A10, Polyclonal Antibody (Cat# AAA19162)

Full Name
Anti-S100A10 Picoband antibody
Gene Names
S100A10; 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
product-image-AAA19151_IHC6.jpg IHC (Immunohistchemistry) (Figure 6. IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (AAA19151).MAP1LC3A was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP1LC3A Antibody (AAA19151) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MAP1LC3A, Polyclonal Antibody (Cat# AAA19151)

Full Name
Anti-MAP1LC3A Picoband antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
product-image-AAA19328_FCM6.png FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U937 cells using anti-BDH1 antibody (AAA19328).Overlay histogram showing U937 cells stained with AAA19328 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BDH1 Antibody (AAA19328,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

BDH1, Polyclonal Antibody (Cat# AAA19328)

Full Name
Anti-BDH1 Antibody
Gene Names
BDH1; BDH; SDR9C1
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19382_FCM11.png FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of RH35 cells using anti- eRF1/ETF1 antibody (AAA19382).Overlay histogram showing RH35 cells stained with AAA19382 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-eRF1/ETF1 Antibody (AAA19382, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

eRF1/ETF1, Monoclonal Antibody (Cat# AAA19382)

Full Name
Anti-eRF1/ETF1 Antibody (monoclonal, 3B6)
Gene Names
ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19276_FCM12.jpg FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of U937 cells using anti-DYNLL1/PIN antibody (AAA19276).Overlay histogram showing U937 cells stained with AAA19276 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DYNLL1/PIN Antibody (AAA19276, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

DYNLL1/PIN, Polyclonal Antibody (Cat# AAA19276)

Full Name
Anti-DYNLL1/PIN Antibody
Gene Names
DYNLL1; LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19381_IF11.jpg IF (Immunofluorescence) (Figure 11. IF analysis of Tubulin alpha using anti- Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

Tubulin alpha, Monoclonal Antibody (Cat# AAA19381)

Full Name
Anti-Tubulin alpha Antibody (monoclonal, 7B12)
Gene Names
TUBA1A; LIS3; TUBA3; B-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19142_IHC8.jpg IHC (Immunohistochemistry) (Figure 8. IHC analysis of HBD using anti-HBD antibody (AAA19142).HBD was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HBD Antibody (AAA19142) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

HBD, Polyclonal Antibody (Cat# AAA19142)

Full Name
Anti-HBD Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
product-image-AAA11918_APP10.jpg Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

CD8 ALPHA, Monoclonal Antibody (Cat# AAA11918)

Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence
Pricing
product-image-AAA31344_APP10.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

alpha Tubulin, Polyclonal Antibody (Cat# AAA31344)

Full Name
Acetyl-alpha Tubulin (Lys40) Antibody
Gene Names
TUBA1B; K-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA12069_APP10.jpg Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

CD8 ALPHA, Monoclonal Antibody (Cat# AAA12069)

Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence
Pricing
product-image-AAA13868_IF3.jpg IF (Immunofluorescence) (Immunofluorescence – anti-Tubulin alpha4A Ab - Microtubule Marker in mouse embryonic fibrolasts at 1/50 dilution;)

TUBA4A, Polyclonal Antibody (Cat# AAA13868)

Full Name
Tubulin Alpha4A Chain Polyclonal Antibody
Gene Names
TUBA4A; TUBA1; H2-ALPHA
Reactivity
Reacts against human, rat, mouse, canine and monkey proteins.
Applications
Immunofluorescence, Western Blot
Pricing
product-image-AAA29642_IHC6.jpg IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

NFkappaB p65, Monoclonal Antibody (Cat# AAA29642)

Full Name
NFkappaB p65 Mouse Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification using immunogen.
Pricing
product-image-AAA29643_IHC8.jpg IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue.1,-tubulin Monoclonal Antibody(8F11) was diluted at 1:200 (4C,overnight).2, Sodium citrate pH 6.0 was used for antibody retrieval (>98C,20min).3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)

alpha-tubulin, Monoclonal Antibody (Cat# AAA29643)

Full Name
alpha-tubulin Mouse Monoclonal Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Affinity purification using immunogen.
Pricing
product-image-AAA31050_IHC11.jpg IHC (Immunohistochemistry) (AAA31050 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

ZAP-70, Polyclonal Antibody (Cat# AAA31050)

Full Name
Phospho-ZAP-70 (Tyr319) Antibody
Gene Names
ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31070_APP10.jpg Application Data (The involvement of AMPK and Akt in the negative regulation of mTOR by APPL1 in STZ-induced diabetic rats.The variations of p-AMPK and p-Akt in normal control rats with vacant lentiviral vector control, normal control rats with APPL1 knockdown, PDN rats, and PDN rats with APPL1 knockdown, and PDN rats with APPL1 overexpression.The abbreviations for the groups of negative control, normal control + shRNA encoding APPL1, painful diabetic neuropathy (PDN), PDN + shRNA encoding APPLL (shRNA) and PDN + APPL1 genetic overexpression are shown as CON, CON + shRNA, PDN, PDN + shRNA, and PDN + OXP. (n = 4, *P < 0.05 vs. NC group, # P < 0.05 vs. PDN group).Data are expressed as the means ± SEM.)

Phospho-AMPK1 (Ser486), Polyclonal Antibody (Cat# AAA31070)

Full Name
Phospho-AMPK1 (Ser486) Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
product-image-AAA13839_IF5.jpg IF (Immunofluorescence) (Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite’ Mounting Medium with DAPI (nuclei, blue).)

CD3, Monoclonal Antibody (Cat# AAA13839)

Full Name
CD3 (T-Cell Marker) Mouse Monoclonal Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human, Mouse, Rat
Applications
Functional Assay, Flow Cytometry, Immunofluorescence
Pricing
product-image-AAA28058_IP20.jpg IP (Immunoprecipitation) (Immunoprecipitating TUBB in Hela whole cell lysateLane 1: Mouse control IgG instead in Hela whole cell lysate.Lane 2: (2ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (5ug)For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:5000)

TUBB, Monoclonal Antibody (Cat# AAA28058)

Full Name
TUBB Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
product-image-AAA31115_IF5.jpg IF (Immunofluorescence) (AAA31115 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IL11RA, Polyclonal Antibody (Cat# AAA31115)

Full Name
IL11RA Antibody
Gene Names
IL11RA; CRSDA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
product-image-AAA30934_IHC12.jpg IHC (Immunohistochemistry) (AAA30934 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

I kappaB alpha, Polyclonal Antibody (Cat# AAA30934)

Full Name
Phospho-I kappaB alpha (Ser32/Ser36) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA30994_IHC6.jpg IHC (Immunohistchemistry) (AAA30994 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IGF1R, Polyclonal Antibody (Cat# AAA30994)

Full Name
Phospho-IGF1R (Tyr1161) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA30949_IHC7.jpg IHC (Immunohistochemistry) (AAA30949 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IKK alpha, Polyclonal Antibody (Cat# AAA30949)

Full Name
Phospho-IKK alpha (Ser176)/IKK beta (Ser177) Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing

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