Search results for "Family Laminin"

2 results for "Family Laminin" - showing 1-2

Display

< >

<< Previous Next >>

WB (Western Blot) (Sample: Recombinant GAL3, Human; Antibody: Rabbit Anti-Human GAL3 Ab)

SDS-PAGE (Sample: Active recombinant GAL3, Human)

Gene Sequencing (extract)

Hemagglutination assay (Figure 3. The hemagglutination assay of GAL3 in V- bottom shaped 96-well microtiter plate.)

Effective concentration of GAL3 (Figure 2.GAL3 also can agglutinate red blood. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL3 with 0.01M PBS (pH7.4), add 50uL a serial dilution of GAL3 to each well of a U or V- bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL3, replace with 50uL 0.01M PBS. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 1-2 hours at room temperature. The results are shown in Figure 2. It was obvious that the minimal effective concentration of GAL3 is 1.2ug/mL.)

Activity (Figure 1.Galectin 3 (GAL3) is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. The protein also has been demonstrated to be involved in cancer, inflammation and fibrosis, heart disease, and stroke. GAL3 is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Besides, Alpha-2-Heremans Schmid Glycoprotein (aHSG) has been identified as an interactor of GAL3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human GAL3 and recombinant human aHSG. Briefly, GAL3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to aHSG-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GAL3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of GAL3 and aHSG was shown in Figure 1, and this effect was in a dose dependent manner.)

Sequence Information

Galectin 3 (GAL3), Active Protein (Cat# AAA21140)

Full Name

Active Galectin 3 (GAL3)

Gene Names

LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>98%

Pricing

< >

<< Previous Next >>

Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IF (Immunofluorescence) (Immunofluorescent staining of paraformaldehyde-Raji cells using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).)

FCM (Flow Cytometry) (Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).)

SDS-PAGE (SDS-PAGE Analysis Purified Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699). Confirmation of Integrity and Purity of Antibody)

WB (Western Blot) (Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IF (Immunofluorescence) (Immunofluorescent staining of HeLa cells using Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Laminin Receptor Monospecific Mouse Monoclonal Antibody (RPSA/2699).)

Laminin Receptor/RPSA, Monoclonal Antibody (Cat# AAA23908)

Full Name

Laminin Receptor/RPSA (Marker of Metastatic Potential)

Gene Names

RPSA; SA; LBP; LRP; p40; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4

Reactivity

Human. Others not known.

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

Loading...

Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

2 results for "Family Laminin" - showing 1-2

Galectin 3 (GAL3), Active Protein (Cat# AAA21140)

Laminin Receptor/RPSA, Monoclonal Antibody (Cat# AAA23908)

Loading...

Need a datasheet, manual, COA, or MSDS? Have a question?

Interested to get a quote about our products?

Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

2 results for "Family Laminin" - showing 1-2

Galectin 3 (GAL3), Active Protein (Cat# AAA21140)

Laminin Receptor/RPSA, Monoclonal Antibody (Cat# AAA23908)

Submit a Question

Request more Information

Request a Manual

Request a Quote