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FCM (Flow Cytometry) (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335 detected with Goat anti Mouse IgG (H/L):FITC (STAR117F), and Mouse anti Pig wCD8a:RPE)

Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by flow cytometry.Image caption: NK cell numbers in the blood of influenza infected pigs. Blood was taken from influenza A virus infected (n = 12) and control pigs (n = 9) on day 0-3 pi in a second experiment. (A) Isolated PBMCs were analysed by flow cytometry and live CD3- lymphocytes were gated as NKp46- or NKp46+ NK cells according to CD8a and NKp46 expression. Plots are taken from a representative control animal. Absolute numbers of (B) NKp46+ NK cells in PBMC were analysed by flow cytometry in control (left) and infected animals (right). (C) Results for the NKp46+ cells in the two groups were compared on each sampling day. (D) NKp46- NK cells in PBMC in control (left) and infected animals (right). (E) NKp46- NK cells were compared for the two groups. Each line in (B) and (D) represents one animal. **p=0.01.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)

Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescence.Image caption:Staining for apoptosis in the lungs. Lung tissue sections from animals infected with influenza A virus (n = 12) were stained with immunofluorescence markers against (A) NKp46(green), (B) influenza A NP(red) and (C) the apoptosis marker caspase-3(blue). (D) Overlay displaying simultaneously influenza A virus NP+ and caspase-3+ cells as purple (arrows). Representative of virus infected bronchiole at day 1 pi. Immunofluorescence staining, 400x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)

Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescence.Image caption:NKp46+ cells in the lungs of influenza virus infected pigs.Lung tissue sections from pigs infected with influenza A virus and control pigs were stained with immunofluorescence markers for cytokeratin (blue), NKp46 (green) and influenza A virus NP (red). NKp46+ cells were counted in areas were influenza A virus NP was (A) detected and (B) not detected. Representative pictures taken from the same animal on day 1 pi are shown. Arrows point at NKp46+ cells. Immunofluorescence staining, 200x. (C) Plot shows number of NKp46+ cells per 0,1 mm2 in sections (n = 24 per animal) from control animals (n = 6) and in areas with and without virus in infected animals (n = 4 per day) calculated as described in Material and Methods. Groups with different letters differ significantly (p=0.05). (D) NKp46+ cells in the lumen of a bronchus (BL). Arrows point at the epithelial lining. Representative picture of luminal exudate, taken from an infected animal on day 2 pi. Insert shows NKp46+ and influenza A virus NP+ cell in the lung tissue of an infected animal on day 1 pi. Immunofluorescence staining, 400x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)

Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by flow cytometry.Image caption: Percentages of NK cells and expression of NKp46 and CD25 in lung tissue. Mononuclear cells were isolated from lung tissue of pigs infected with influenza A virus (n = 12) and control animals (n = 6) during the first 3 days pi and analysed by flow cytometry. (A) Live CD3- lymphocytes were gated as described in Fig 2. NK cells were gated according to CD8a and NKp46 expression and defined as NKp46-, NKp46int or NKp46high cells. Plot shown is from a representative control animal. (B) Proportions of NKp46- (green), NKp46int (blue) and NKp46high (purple) NK cells in individual animals, shown as percentages of gated cells in lymphocytes. (C) Percentages of NKp46- (left), NKp46int (middle) and NKp46high (right) NK cells among lymphocytes. (D) Median fluorescence intensity (MFI) in the NKp46- gate (left), the NKp46+ gate (middle) and in the NKp46high gate (right) are shown. (E) CD25+ cells were gated in the NKp46- and NKp46int NK cells (left) and in the NKp46high NK cells (right). Plots shown are from a representative control animal. (F) The percentages of CD25+ cells in each gate were calculated in control animals (green), infected animals from day 1 (purple), day 2 (blue) and day 3 (pink) pi. *p=0,05, **p=0.01.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)

Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescence.Image caption:Counting of NKp46+ cells. Numbers of NKp46+ cells per area were counted in lung tissue sections from control animals and influenza A virus infected animals as described in Material and Methods. Representative picture of area with virus from infected animal. Immunofluorescence staining, 200x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)

Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:Splenic NKp46high NK cells produced the highest levels of IFN-?. (A + B) Intracellular cytokine staining for IFN-? production in NKp46-defined NK cells within PBMC (upper graphs) and splenocytes (lower graphs) by four-colour FCM after 24 h in vitro stimulation with rhIL-2, rpIL-12 and rpIL-18 or medium. CD3- lymphocytes were gated (not shown) and NKp46/CD8a defined total NK cells were further subgated for IFN-? production. IFN-? producing NK cells were gated according to NKp46 expression levels (CD8a+NKp46-: blue, CD8a+NKp46+: green, CD8adim/-NKp46high: red). (A) Numbers indicate the percentage of IFN-?+ NK cells within respective gates. Results are representative for experiments with four different animals. (B) Percentage of IFN-?+ cells (left graph) and mean fluorescence intensity of IFN-?+ cells (right graph) within the different NK-cell subsets in blood and spleen of four animals are shown. (C) Supernatants of cytokine stimulated FACS-sorted CD3-CD8a+NKp46- and CD3-CD8a+NKp46+ NK cells from blood and CD3-CD8a+NKp46-, CD3-CD8a+NKp46+ and CD3-CD8adim/-NKp46high NK cells from spleen were tested for IFN-? production in ELISA following 24 h in vitro stimulation with rhIL-2, rpIL-12 and rpIL-18. Data on the left are from one representative animal and displayed as the mean of duplicates +/- SD. IFN-? production in experiments with four animals analysed is shown on the right. Mean values are represented by a black bar. (B + C) Significant differences between the subsets in blood or spleen are indicated (* = p < 0.05, ** = p < 0.01).From: Mair KH, Mllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmller A, Gerner W. Porcine CD8adim/-NKp46high NK cells are in a highly activated state. Vet Res. 2013 Mar 1;44:13.)

Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:Varying expression of NKp46, CD8a, CD16 and CD27 on NK-cell subsets in blood and spleen. (A) Following five-colour staining, PBMC and splenocytes were gated on CD3- cells and further subgated according to their NKp46/CD8a expression pattern in NKp46- and NKp46+ NK cells in blood (upper graph) and NKp46-, NKp46+ and NKp46high NK cells in spleen (lower graph). (B) 14 healthy 6 -7 month old pigs were investigated for NKp46 and CD8a expression levels in the NKp46-defined NK-cell subsets. Box-plots show the mean fluorescence intensity of the two markers. (C) NK-cell subsets defined in (A) were further analysed for their expression of the surface markers CD16 and CD27. Histograms show the expression of the two markers within the respective NKp46-defined subsets (CD8a+NKp46-: blue histograms, CD8a+NKp46+: green histograms, CD8adim/-NKp46high: red histograms) in blood (upper graphs) and spleen (lower graphs) according to the corresponding isotype control (grey histrograms with dotted lines). Box-plots show the mean fluorescence intensity of CD16 and CD27 of the NKp46-defined NK-cell subsets in blood and spleen of 14 healthy 6 -7 month old pigs. (B + C) Significant differences between the subsets in blood or spleen are indicated (* = p < 0.05, ** = p < 0.01, *** = p < 0.001).From: Mair KH, Mllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmller A, Gerner W. Porcine CD8adim/-NKp46high NK cells are in a highly activated state. Vet Res. 2013 Mar 1;44:13.)

Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335:Alexa Fluor488 and Mouse anti Pig wCD8a:RPE)

Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335:APC and Mouse anti Pig wCD8a:RPE)

CD335, Monoclonal Antibody (Cat# AAA12253)

Full Name

MOUSE ANTI PIG CD335

Reactivity

Pig

Applications

Flow Cytometry, Immunofluorescence

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Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:Morphology and P450 arom immunoreactivity of tumoral region in human testis with seminoma. A-B: Haematoxylin-eosin staining. C-D: Strong P450 arom immunoreactivity in cytoplasm of neoplastic cells (Nc) and unstained lymphocytes (L). Insert: absorption control. Scale bars: A, 20 um; B-C, 12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in rat prostate by immunohistochemistry on paraffin embedded sectionsImage caption:Immunohistochemical staining of aromatase in prostate of control and BPA-treated rats at doses of 25, 50, 300, or 600 ug/Kg/d for 4 days.From: Castro B, S¡nchez P, Torres JM, Preda O, del Moral RG, Ortega E. Bisphenol A exposure during adulthood alters expression of aromatase and 5a-reductase isozymes in rat prostate. PLoS One. 2013;8(2):e55905.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:P450 arom immunoreactivity in testicular region adjacent to seminoma and in controls A: Intense aromatase immunostaining in IGCN cells. B: Placental-like alkaline phosphatase staining of IGCN basal cells C: Strong aromatase immunoreactivity of interstitial Leydig cells in normal testis (Lc) D: Intense immunostaining of luteal cells (Luc) in ovarian tissue Scale bars: A-B, 8 um; C,12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 use for the detection of aromatase in KGN cells by western blottingImage caption:Effect of H89 on aromatase and BRCA1 expression in KGN cells. A, The human ovarian granulosa cell line KGN were treated with control (DMSO), 20 mM H89, 50 mM PD98059, 200 nM Wortmannin, 100 nM Rapamycin 100 nM, and cells were harvested 24 hours after treatment. Immunoblotting was performed using specific antibodies against aromatase, BRCA1 and a-tubulin; B, Aromatase and BRCA1 mRNA levels were measured by real-time RT-PCR. Gapdh was used for normalizing the real time PCR results. The data are expressed as a fold (Aromatase mRNA), or a percentage (BRCA1 mRNA) relative to the DMSO-treated control.From: Ghosh S, Lu Y, Hu Y. A Role of CREB in BRCA1 Constitutive Promoter Activity and Aromatase Basal Expression. Int J Biomed Sci. 2008 Dec 15;4(4):260-265.)

Application Data (Western blot analysis of human placenta extract probed with Mouse anti Human Cytochrome P450 Aromatase followed by F(ab')2 Rabbit anti Mouse IgG:HRP)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in human lung tumors by immunohistochemistry on formalin fixed paraffin embedded tissue.Image caption:Representative immunohistochemistry of lung tumors for ERa, ERbeta, aromatase, EGFR and PR.From: Stabile LP, Dacic S, Land SR, Lenzner DE, Dhir R, Acquafondata M, Landreneau RJ, Grandis JR, Siegfried JM. Combined analysis of estrogen receptor beta-1 and progesterone receptor expression identifies lung cancer patients with poor outcome. Clin Cancer Res. 2011 Jan 1;17(1):154-64.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in porcine spermatozoa by immunofluorescenceImage caption:Immunofluorescence labelling of androgen and estrogen receptors in pig spermatozoa: A) AR red fluorescence in sperm proximal mid-piece. B) P450arom red brilliant light in the proximal tail of sperm with a diffuse labelling in the distal tail. C) ERa red fluorescence in the sperm mid-piece, together with a faint labelling in the tail. D) ERbeta green intense light in the sperm acrosomal region. Inserts: immunonegative absorption controls. Scale bars: 5 um.From: Rago V, Aquila S, Panza R, Carpino A. Cytochrome P450arom, androgen and estrogen receptors in pig sperm. Reprod Biol Endocrinol. 2007 Jun 6;5:23.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in sheep tissues by immunohistochemistry on paraffin sectionsImage caption:a and b) Low- (a) and higher- (b) power sections of the fetal end of a placentome in G1 stained with the prolactin antiserum and showing the intensely stained binucleate cells scattered along the trophoblast, but with a tendency to be more densely accumulated towards the fetal end (scale bars a=200?um; b=150?um). (c) High-power section of the trophoblast -endometrium interface in a placentome in G2 stained with the prolactin antiserum. Note the patchy staining of the thin, elongated endometrial epithelial cells (arrowed) in close contact with the intensely stained binucleate trophoblast cells (scale bar=40?um). (d) Low-power section of the intercotyledonary allantochorion stained with the prolactin antiserum and showing the relatively dense accumulation of binucleate trophoblast cells (arrowed) which remain unstained in this region of the placenta (scale bar=100?um). (e) High-power section at the edge of a placentome of G1 showing positive staining of the uninucleate trophoblast by the 3beta-HSD antiserum. The binucleate cells (arrowed) remain unstained (scale bar=40?um). (f) Section of the distended endometrial glands at the lateral border of a placentome in G2 stained with 3beta-HSD. The basal portions of the endometrial cells lining the glands stain strongly as does the secretory material adhered to the luminal surface of the cells and the accumulated coagulum, possibly as a result of ˜stickiness' of the material (scale bar=150?um). (g) High-power section of the intercotyledonary region of the allantochorion in G1 showing positive staining of the cytoplasm of the uninucleate, but not binucleate trophoblast by the 17,20 lyase antiserum (scale bar=40?um). (h) Section at the fetal end of a placentome from G2 remaining unstained by the aromatase antiserum (scale bar=150?um). (i) Section of near-term sheep placenta showing strong, positive staining of the uninucleate, but not binucleate, trophoblast by the aromatase antiserum (scale bar=150?um). (j) High-power section at the fetal end of a placentome in G1 showing strong positive staining of the uninucleate trophoblast by the PR antiserum. The binucleate cells (arrowed) remain unstained (scale bar=40?um).From: Wilsher S, Stansfield F, Greenwood RE, Trethowan PD, Anderson RA, Wooding FB, Allen WR. Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis). Reproduction. 2013 May 21;145(6):541-54.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in human synovial tissue by immunohistochemistry on cryosectionsImage caption:Aromatase expression in synovial tissue and endogenous steroid hormone release from superfused synovium. (a) Immunohistochemistry of aromatase in one OA and one RA patient. Using the respective control antibody revealed no staining of positive cells (not shown). Magnification: 400x. (b) Density of aromatase-positive cells in OA (open bars, n = 20) and RA patients (hatched bars, n = 16). (c) Spontaneously released E2, E3, and free testosterone from standardized superfused pieces of synovial tissue of OA (open bars, n = 20) and RA patients (hatched bars, n = 18). (b,c) Values are given as box blots with the 5th, 25th, 50th (median), 75th, and 95th percentile when applicable. OA, osteoarthritis; RA, rheumatoid arthritis. Other abbreviations are as given in the legend to Fig. 1.From: Schmidt M, Weidler C, Naumann H, Anders S, Sch¶lmerich J, Straub RH. Androgen conversion in osteoarthritis and rheumatoid arthritis synoviocytes--androstenedione and testosterone inhibit estrogen formation and favor production of more potent 5alpha-reduced androgens. Arthritis Res Ther. 2005;7(5):R938-48.)

CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)

Full Name

MOUSE ANTI HUMAN CYTOCHROME P450 AROMATASE

Gene Names

CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

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2 results for "Family F Box" - showing 1-2

CD335, Monoclonal Antibody (Cat# AAA12253)

CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)

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2 results for "Family F Box" - showing 1-2

CD335, Monoclonal Antibody (Cat# AAA12253)

CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)

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