Search results for "Control pAb"

< >

<< Previous Next >>

WB (Western Blot) (Sample: Recombinant GAL3, Human; Antibody: Rabbit Anti-Human GAL3 Ab)

Galectin 3 (GAL3), Active Protein (Cat# AAA21140)

Full Name

Active Galectin 3 (GAL3)

Gene Names

LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>98%

Pricing

< >

Lamin A/C, Polyclonal Antibody (Cat# AAA28349)

Full Name

[KO Validated] Lamin A/C Rabbit pAb

Gene Names

LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody (Center). AAA28746 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

HIST1H2AG, Polyclonal Antibody (Cat# AAA28746)

Full Name

HIST1H2AG Antibody (Center)

Gene Names

HIST1H2AI; H2A/c; H2AFC

Reactivity

Human (Predicted Reactivity: Rat, Mouse, Bovine, Xenopus, Yeast, Chicken, Monkey, Zebrafish, Drosophila)

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

GAPDH, Polyclonal Antibody (Cat# AAA28693)

Full Name

GAPDH Antibody (C-term R248)

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

ATP5B, Polyclonal Antibody (Cat# AAA28724)

Full Name

ATP5B Antibody (Center)

Gene Names

ATP5B; ATPMB; ATPSB; HEL-S-271

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Rat)

Applications

Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from Hela, HepG2, MCF-7, SH-SY5Y, mouse NIH/3T3 cell line (from left to right), using FGFR1 Antibody (Center). AAA28687 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

FGFR1, Polyclonal Antibody (Cat# AAA28687)

Full Name

FGFR1 Antibody (Center)

Gene Names

FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1

Reactivity

Human, mouse (Predicted Reactivity: Chicken, Rat)

Applications

Flow Cytometry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (PCNA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

PCNA, Polyclonal Antibody (Cat# AAA28705)

Full Name

PCNA Antibody (C-term)

Gene Names

PCNA; ATLD2

Reactivity

Human (Predicted Reactivity: Bovine, Hamster, Monkey, Rat)

Applications

Immunohistochemistry, Immunofluorescence, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SOCS1, Polyclonal Antibody (Cat# AAA28752)

Full Name

SOCS1 Antibody (N-term)

Gene Names

SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1

Reactivity

Mouse, rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (HAS2 Antibody (Center) (AAA28714) flow cytometric analysis of K562 cells (bottom histogram) compared to a Rabbit IgG Isotype control (negative control-top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

HAS2, Polyclonal Antibody (Cat# AAA28714)

Full Name

HAS2 Antibody (Center)

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Vimentin, Polyclonal Antibody (Cat# AAA28770)

Full Name

Vimentin Antibody

Gene Names

VIM; HEL113; CTRCT30

Reactivity

Human

Applications

Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (CDK4 Antibody (C-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

CDK4, Polyclonal Antibody (Cat# AAA28747)

Full Name

CDK4 Antibody (C-term)

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)

S100B, Polyclonal Antibody (Cat# AAA28759)

Full Name

S100B Antibody

Gene Names

S100B; NEF; S100; S100-B; S100beta

Reactivity

Human, Mouse, Rat (Predicted: Rabbit)

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

WB (Western Blot) (CYK18 Antibody (C-term) Western blot of A431 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow) (Kindly offered by Dr. Li).)

KRT18 / CK18 / Cytokeratin 18, Polyclonal Antibody (Cat# AAA12370)

Full Name

Rabbit Polyclonal to Human KRT18 / CK18 / Cytokeratin 18

Gene Names

KRT18; K18; CYK18

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry

Purity

Ammonium sulfate precipitation

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Flow cytometric analysis of SK-BR-3 cells using MICA Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). AAA28710 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

MICA, Polyclonal Antibody (Cat# AAA28710)

Full Name

MICA Antibody (Center)

Gene Names

MICA; MIC-A; PERB11.1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.)

HLA-B, Polyclonal Antibody (Cat# AAA28743)

Full Name

HLA-B Antibody (N-term)

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using HSP27/HSP27/HSPB1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

HSP27/HSPB1, Polyclonal Antibody (Cat# AAA28328)

Full Name

[KO Validated] HSP27/HSPB1 Rabbit pAb

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using SIRT1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

SIRT1, Polyclonal Antibody (Cat# AAA28348)

Full Name

[KO Validated] SIRT1 Rabbit pAb

Gene Names

SIRT1; SIR2L1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of NOS2A Antibody (Center) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

NOS2A, Polyclonal Antibody (Cat# AAA28780)

Full Name

NOS2A Antibody (Center)

Gene Names

NOS2; NOS; INOS; NOS2A; HEP-NOS

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using WNT10B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WNT10B, Polyclonal Antibody (Cat# AAA28334)

Full Name

WNT10B Rabbit pAb

Gene Names

WNT10B; SHFM6; WNT-12

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of SRC Antibody (N-term) with A431 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

SRC, Polyclonal Antibody (Cat# AAA28721)

Full Name

SRC Antibody (N-term)

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

CD38, Polyclonal Antibody (Cat# AAA28702)

Full Name

CD38 Antibody (C-term)

Gene Names

CD38; T10; ADPRC 1

Reactivity

Human (Predicted Reactivity: Monkey)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Sample: Recombinant PRF1, Human;Antibody: Rabbit Anti-Human PRF1 Ab)

Activity (Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. Besides, Calreticulin (CRT) has been identified as an interactor of PRF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PRF1 and recombinant human CRT. Briefly, PRF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CRT-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PRF1 and CRT was shown in Figure 1, and this effect was in a dose dependent manner.The activity of recombinant PRF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant human PRF1 with 0.9% NaCl, add 50uL a serial dilution of PRF1, 10uL 0.1M CaCl2 to each well, then add 50uL 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 10uL 0.9% NaCl to reaplace CaCl2 in control wells. The plate is incubated for 20 hours at 37 degree C, 5% CO2. The results are shown in Figure 2. It was obvious that the minimal effective concentration of PRF1 is 12.5ug/mL.)

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

Full Name

Active Perforin 1 (PRF1)

Gene Names

PRF1; P1; PFP; HPLH2

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>80%

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of CYK18 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

CYK18, Polyclonal Antibody (Cat# AAA28709)

Full Name

CYK18 Antibody (C-term)

Gene Names

KRT18; K18; CYK18

Reactivity

Human, mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using Bax Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.)

Bax, Polyclonal Antibody (Cat# AAA28318)

Full Name

Bax Rabbit pAb

Gene Names

BAX; BCL2L4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (HSPA5 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

HSPA5, Polyclonal Antibody (Cat# AAA28738)

Full Name

HSPA5 Antibody

Gene Names

HSPA5; BIP; MIF2; GRP78; HEL-S-89n

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IDH1, Polyclonal Antibody (Cat# AAA28676)

Full Name

IDH1 Antibody (Center)

Gene Names

IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26

Reactivity

Human, mouse (Predicted Reactivity: Bovine, Rat)

Applications

Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of CTNNB1 Antibody (C-term) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

CTNNB1, Polyclonal Antibody (Cat# AAA28720)

Full Name

CTNNB1 Antibody (C-term)

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human (Predicted Reactivity: Bovine, Mouse, Rat)

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

Calreticulin, Polyclonal Antibody (Cat# AAA28363)

Full Name

[KO Validated] Calreticulin Rabbit pAb

Gene Names

CALR; RO; CRT; SSA; cC1qR

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with XRCC6 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with XRCC6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). XRCC6 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)

XRCC6, Polyclonal Antibody (Cat# AAA28768)

Full Name

XRCC6 Antibody (C-term)

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of SK-Br-3 cells using PTAR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

PTAR1, Polyclonal Antibody (Cat# AAA28765)

Full Name

PTAR1 Antibody (Center)

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (SEPT9 Antibody (A555) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SEPT9, Polyclonal Antibody (Cat# AAA28793)

Full Name

SEPT9 Antibody (C-term)

Gene Names

SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

SIRT3, Polyclonal Antibody (Cat# AAA28346)

Full Name

SIRT3 Rabbit pAb

Gene Names

SIRT3; SIR2L3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CDK4 antibody . Western blot was performed from the immunoprecipitate using CDK4 antibody at a dilition of 1:1000.)

CDK4, Polyclonal Antibody (Cat# AAA28337)

Full Name

CDK4 Rabbit pAb

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (TFAM Antibody (C-term) western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the TFAM antibody detected the TFAM protein (arrow).)

TFAM, Polyclonal Antibody (Cat# AAA28696)

Full Name

TFAM Antibody (C-term)

Gene Names

TFAM; TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat heart using IRS1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IRS1, Polyclonal Antibody (Cat# AAA28342)

Full Name

IRS1 Rabbit pAb

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of LIN28B Antibody (N-term) in HL60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified Pab.)

LIN28B, Polyclonal Antibody (Cat# AAA12320)

Full Name

Rabbit Polyclonal to Human LIN28B

Gene Names

LIN28B; CSDD2

Reactivity

Human

Applications

Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry

Purity

Ammonium sulfate precipitation

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.)

BHLH3, Polyclonal Antibody (Cat# AAA28734)

Full Name

BHLH3 Antibody (N-term)

Gene Names

BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunofluorescence, Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using p70 S6 Kinase 1 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

p70 S6 Kinase 1, Polyclonal Antibody (Cat# AAA28344)

Full Name

p70 S6 Kinase 1 Rabbit pAb

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Parkin, Polyclonal Antibody (Cat# AAA28668)

Full Name

Parkin Antibody (C-term)

Gene Names

PARK2; PDJ; PRKN; AR-JP; LPRS2

Reactivity

Human, mouse

Applications

WB, EIA, IF, FC/FACS, IHC

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for binding efficiency determinationImage caption:Binding efficiencies (BE) of different a4beta7 molecules composed of distinct a4 mutants to monoclonal antibodies against a4, beta7 or the a4beta7 heterodimer. Binding efficiency is determined by the ratio between the mean fluorescence of antibody binding to each a4 molecule and of the binding in a mock-transfected cell culture (see Materials and Methods for details). Dark gray bars represent binding to the human (wild type) a4 clone, whereas light gray bars are those of binding to the different a4 mutants (as shown in the x-axis). a4 mutants which included substitutions at codon 201 are boxed. A, binding of anti-a4 2b4 antibody. B, binding of anti-a4 HP2/1 antibody. C, BE of different anti-a4 and beta7 antibodies to the human a4 and the quintuple a4 mutant (5 aa mut). Bars represent the range of standard errors deduced from triplicate experiments. p-values of Student's t tests are shown above each comparison. NS, non-significant (> 0.05).From:Darc M, Hait SH, Soares EA, Cicala C, Seuanez HN, et al. (2011) Polymorphisms in the a4 Integrin of Neotropical Primates: Insights for Binding of Natural Ligands and HIV-1 gp120 to the Human a4beta7. PLoS ONE 6(9): e24461.)

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for western blotting and immunoprecipitationImage caption:Tetraspanins CD81, CD82 and CD151 are associated with a4beta1 throughout erythroid maturation and with beta3 in proerythroblasts and basophilic erythroblasts. A. CD81, CD82 and CD151 precipitates from Mn2+-activated proerythroblasts (ProEB, day 5), basophilic (BasoEB, day 8) and polychromatic (PolyEB, day 12) erythroblasts were successively probed with anti-a4, anti-beta1 and anti-beta3 antibodies; tetraspanin controls from each time point are also illustrated. All tetraspanins co-precipitated a4 and beta1 from erythroblasts B. Tetraspanin precipitates from day 6 proerythroblasts (ProEB) solubilised in the presence of EDTA or different cations, and from Mn2+-activated basophilic erythroblasts (BasoEB, day 8) were probed with a mix of antibodies to a5, beta1, beta2 and beta3 integrins while the control samples were probed with the relevant tetraspanin antibodies. For clarity, integrin controls are illustrated for the EDTA blot but were present on all blots. beta1 and beta3 integrins were precipitated well only in the presence of Mn2+. C. CD81 and CD82 precipitates from day 5 proerythroblasts were successively probed with different anti-integrin subunit antibodies and demonstrate co-precipitation of beta1 and beta3 but not a5 or beta2 integrins. D. CD81 (454720) and CD82 (53H5) precipitates from day 6 proerythroblasts (ProEB) and HEL cells (HEL) solubilised in the presence of EDTA, Ca2++Mg2+ or Mn2+ probed with anti-CD82 and anti-CD81 antibodies. Each tetraspanin co-precipitates the other most strongly in the presence of Mn2+ from proerythroblasts while any cation permits co-precipitation in HEL cells. Integrins were analysed on 7.5% gels, tetraspanins on 12% gels; non-reducing conditions. Unless stated, the following clones were used: CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; a5, IIA1; aL, TS1/22; aIIb, PAB-1. All day 5 and 6 cultures comprised 90 -95% proerythroblasts; day 8 culture comprised 5% proerythroblasts, 81% basophilic erythroblasts and 14% polychromatic erythroblasts; day 12 culture comprised 41% polychromatic erythroblasts, 15% orthochromatic erythroblasts and 41% reticulocytes. In the day 5 and 6 cultures 15 -34% of cells were GPA+ and 28 -35% of cells were aIIb+. Day 8 and day 12 cultures had 77% and 97% GPA+ cells, respectively, and 9% and 0% aIIb+ cells, respectively.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4beta1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1. PLoS ONE 8(5): e62654.)

CD49d, Monoclonal Antibody (Cat# AAA12003)

Full Name

MOUSE ANTI HUMAN CD49d

Gene Names

ITGA4; IA4; CD49D

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (AGXT Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

AGXT, Polyclonal Antibody (Cat# AAA28732)

Full Name

AGXT Antibody (Center)

Gene Names

AGXT; AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ITCH antibody at dilution of 1:100 (40x lens).)

ITCH, Polyclonal Antibody (Cat# AAA28315)

Full Name

[KO Validated] ITCH Rabbit pAb

Gene Names

ITCH; AIF4; AIP4; NAPP1; dJ468O1.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain using CDK6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CDK6, Polyclonal Antibody (Cat# AAA28330)

Full Name

CDK6 Rabbit pAb

Gene Names

CDK6; PLSTIRE

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (SYP Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SYP, Polyclonal Antibody (Cat# AAA28723)

Full Name

SYP Antibody (C-term)

Gene Names

SYP; MRX96; MRXSYP

Reactivity

Human, mouse (Predicted Reactivity: Rat)

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

Vimentin, Monoclonal Antibody (Cat# AAA28380)

Full Name

[KO Validated] Vimentin Rabbit mAb

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (GTF2I Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

GTF2I, Polyclonal Antibody (Cat# AAA28707)

Full Name

GTF2I Antibody (C-term)

Gene Names

GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I

Reactivity

Human (Predicted Reactivity: Rat)

Applications

Immunofluorescence, Immunohistochemistry, Flow Cytometry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for binding efficiency determinationImage caption:Binding efficiencies (BE) of different a4beta7 molecules composed of distinct a4 mutants to monoclonal antibodies against a4, beta7 or the a4beta7 heterodimer. Binding efficiency is determined by the ratio between the mean fluorescence of antibody binding to each a4 molecule and of the binding in a mock-transfected cell culture (see Materials and Methods for details). Dark gray bars represent binding to the human (wild type) a4 clone, whereas light gray bars are those of binding to the different a4 mutants (as shown in the x-axis). a4 mutants which included substitutions at codon 201 are boxed. A, binding of anti-a4 2b4 antibody. B, binding of anti-a4 HP2/1 antibody. C, BE of different anti-a4 and beta7 antibodies to the human a4 and the quintuple a4 mutant (5 aa mut). Bars represent the range of standard errors deduced from triplicate experiments. p-values of Student's t tests are shown above each comparison. NS, non-significant (> 0.05).From:Darc M, Hait SH, Soares EA, Cicala C, Seuanez HN, et al. (2011) Polymorphisms in the a4 Integrin of Neotropical Primates: Insights for Binding of Natural Ligands and HIV-1 gp120 to the Human a4beta7. PLoS ONE 6(9): e24461.)

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for western blotting and immunoprecipitationImage caption:Tetraspanins CD81, CD82 and CD151 are associated with a4beta1 throughout erythroid maturation and with beta3 in proerythroblasts and basophilic erythroblasts. A. CD81, CD82 and CD151 precipitates from Mn2+-activated proerythroblasts (ProEB, day 5), basophilic (BasoEB, day 8) and polychromatic (PolyEB, day 12) erythroblasts were successively probed with anti-a4, anti-beta1 and anti-beta3 antibodies; tetraspanin controls from each time point are also illustrated. All tetraspanins co-precipitated a4 and beta1 from erythroblasts B. Tetraspanin precipitates from day 6 proerythroblasts (ProEB) solubilised in the presence of EDTA or different cations, and from Mn2+-activated basophilic erythroblasts (BasoEB, day 8) were probed with a mix of antibodies to a5, beta1, beta2 and beta3 integrins while the control samples were probed with the relevant tetraspanin antibodies. For clarity, integrin controls are illustrated for the EDTA blot but were present on all blots. beta1 and beta3 integrins were precipitated well only in the presence of Mn2+. C. CD81 and CD82 precipitates from day 5 proerythroblasts were successively probed with different anti-integrin subunit antibodies and demonstrate co-precipitation of beta1 and beta3 but not a5 or beta2 integrins. D. CD81 (454720) and CD82 (53H5) precipitates from day 6 proerythroblasts (ProEB) and HEL cells (HEL) solubilised in the presence of EDTA, Ca2++Mg2+ or Mn2+ probed with anti-CD82 and anti-CD81 antibodies. Each tetraspanin co-precipitates the other most strongly in the presence of Mn2+ from proerythroblasts while any cation permits co-precipitation in HEL cells. Integrins were analysed on 7.5% gels, tetraspanins on 12% gels; non-reducing conditions. Unless stated, the following clones were used: CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; a5, IIA1; aL, TS1/22; aIIb, PAB-1. All day 5 and 6 cultures comprised 90 -95% proerythroblasts; day 8 culture comprised 5% proerythroblasts, 81% basophilic erythroblasts and 14% polychromatic erythroblasts; day 12 culture comprised 41% polychromatic erythroblasts, 15% orthochromatic erythroblasts and 41% reticulocytes. In the day 5 and 6 cultures 15 -34% of cells were GPA+ and 28 -35% of cells were aIIb+. Day 8 and day 12 cultures had 77% and 97% GPA+ cells, respectively, and 9% and 0% aIIb+ cells, respectively.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4beta1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1. PLoS ONE 8(5): e62654.)

CD49d, Monoclonal Antibody (Cat# AAA12002)

Full Name

MOUSE ANTI HUMAN CD49d

Gene Names

ITGA4; IA4; CD49D

Reactivity

Bovine, Cat, Cynomolgus monkey, Goat, Horse, Llama, Mink, Mustelid, Pig, Rabbit, Rat, Rhesus Monkey

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (PROX-1-S514 Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

PROX-1-S514, Polyclonal Antibody (Cat# AAA28650)

Full Name

PROX-1-S514 Antibody

Reactivity

Human, mouse

Applications

WB, EIA, IHC, FC/FACS

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

ID1, Polyclonal Antibody (Cat# AAA28652)

Full Name

ID1 Antibody (Center)

Gene Names

ID1; ID; bHLHb24

Reactivity

Human, mouse (Predicted Reactivity: Rat)

Applications

WB, EIA, IHC, IF, FC/FACS

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using [KO Validated] JAK1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

JAK1, Polyclonal Antibody (Cat# AAA28369)

Full Name

[KO Validated] JAK1 Rabbit pAb

Gene Names

JAK1; JTK3; JAK1A; JAK1B

Reactivity

Human, Mouse, Rat

Applications

WB, IF

Purity

Affinity purification

Pricing

Loading...

Need a datasheet, manual, COA, or MSDS? Have a question?

Interested to get a quote about our products?

Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

81 results for "Control pAb" - showing 1-50

Galectin 3 (GAL3), Active Protein (Cat# AAA21140)

Lamin A/C, Polyclonal Antibody (Cat# AAA28349)

HIST1H2AG, Polyclonal Antibody (Cat# AAA28746)

GAPDH, Polyclonal Antibody (Cat# AAA28693)

ATP5B, Polyclonal Antibody (Cat# AAA28724)

FGFR1, Polyclonal Antibody (Cat# AAA28687)

PCNA, Polyclonal Antibody (Cat# AAA28705)

SOCS1, Polyclonal Antibody (Cat# AAA28752)

HAS2, Polyclonal Antibody (Cat# AAA28714)

Vimentin, Polyclonal Antibody (Cat# AAA28770)

CDK4, Polyclonal Antibody (Cat# AAA28747)

S100B, Polyclonal Antibody (Cat# AAA28759)

KRT18 / CK18 / Cytokeratin 18, Polyclonal Antibody (Cat# AAA12370)

MICA, Polyclonal Antibody (Cat# AAA28710)

HLA-B, Polyclonal Antibody (Cat# AAA28743)

HSP27/HSPB1, Polyclonal Antibody (Cat# AAA28328)

SIRT1, Polyclonal Antibody (Cat# AAA28348)

NOS2A, Polyclonal Antibody (Cat# AAA28780)

WNT10B, Polyclonal Antibody (Cat# AAA28334)

SRC, Polyclonal Antibody (Cat# AAA28721)

CD38, Polyclonal Antibody (Cat# AAA28702)

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

CYK18, Polyclonal Antibody (Cat# AAA28709)

Bax, Polyclonal Antibody (Cat# AAA28318)

HSPA5, Polyclonal Antibody (Cat# AAA28738)

IDH1, Polyclonal Antibody (Cat# AAA28676)

CTNNB1, Polyclonal Antibody (Cat# AAA28720)

Calreticulin, Polyclonal Antibody (Cat# AAA28363)

XRCC6, Polyclonal Antibody (Cat# AAA28768)

PTAR1, Polyclonal Antibody (Cat# AAA28765)

SEPT9, Polyclonal Antibody (Cat# AAA28793)

SIRT3, Polyclonal Antibody (Cat# AAA28346)

CDK4, Polyclonal Antibody (Cat# AAA28337)

TFAM, Polyclonal Antibody (Cat# AAA28696)

IRS1, Polyclonal Antibody (Cat# AAA28342)

LIN28B, Polyclonal Antibody (Cat# AAA12320)

BHLH3, Polyclonal Antibody (Cat# AAA28734)

p70 S6 Kinase 1, Polyclonal Antibody (Cat# AAA28344)

Parkin, Polyclonal Antibody (Cat# AAA28668)

CD49d, Monoclonal Antibody (Cat# AAA12003)

AGXT, Polyclonal Antibody (Cat# AAA28732)

ITCH, Polyclonal Antibody (Cat# AAA28315)

CDK6, Polyclonal Antibody (Cat# AAA28330)

SYP, Polyclonal Antibody (Cat# AAA28723)

Vimentin, Monoclonal Antibody (Cat# AAA28380)

GTF2I, Polyclonal Antibody (Cat# AAA28707)

CD49d, Monoclonal Antibody (Cat# AAA12002)

PROX-1-S514, Polyclonal Antibody (Cat# AAA28650)

ID1, Polyclonal Antibody (Cat# AAA28652)

JAK1, Polyclonal Antibody (Cat# AAA28369)

Submit a Question

Request more Information

Request a Manual

Request a Quote