Search results for "Cell Viability Assay"

5 results for "Cell Viability Assay" - showing 1-5

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WB (Western Blot) (Western BlotSample: Recombinant GAL9, Human;Antibody: Rabbit Anti-Human GAL9 Ab)

SDS-Page (SDS-PAGESample: Active recombinant GAL9, Human)

Application Data (Gene Sequencing (extract))

Application Data (Inhibition of Jurkat cells proliferation after stimulated with GAL9.)

Application Data (GAL9 also can induce cellular apoptosis. To test the effect of GAL9 on cell apoptosis, Jurkat cells were seeded into 96-well plates at a density of 5,000 cells/well with 2% serum standard RPMI 1640 including various concentrations of recombinant human GAL9. After incubated for 48 h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 20 uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 4 h at 37 degree C. Proliferation of Jurkat cells after incubation with GAL9 for 48 h observed by inverted microscope was shown in Figure 3. Cell viability was assessed by CCK-8 assay after incubation with recombinant human GAL9 for 48 h. The result was shown in Figure 4. It was obvious that GAL9 significantly inhibit cell viability of Jurkat cells. The ED50 is 1.64 ug/mL.Inhibition of Jurkat cells proliferation after stimulated with GAL9(A) Jurkat cells cultured in RPMI 1640, stimulated with 2.5ug/mL GAL9 for 48h;(B) Unstimulated Jurkat cells cultured in RPMI 1640 for 48h.)

Application Data (The hemagglutination assay of GAL9 in V- bottom shaped 96-well microtiter plate)

Activity (Galectin 9 (GAL9) is a member of the Beta-galactoside-binding galectin family. Galectin-9 is found outside of cells and may be exported by non-classical pathways. Galectin 9 exhibits a variety of biological activities, the majority of which have focused on its immunomodulatory role toward lymphocytes, were it shows specific interactions with TIM-3, and can negatively regulate Th1 type immunity. It also can agglutinate red blood. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL9 with 0.01M PBS(pH7.4), add 50ul a serial dilution of GAL9 to each well of a U or V- bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL9, replace with 50uL 0.01M PBS. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 1-2 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL9 is 1.5ug/mL.Figure 1.The hemagglutination of recombinant human GAL9(A) Rabbit erythrocyte agglutinated by recombinant human GAL9;(B) Rabbit erythrocyte without recombinant human GAL9.)

Sequence Information

Galectin 9 (GAL9), Active Protein (Cat# AAA21103)

Full Name

Active Galectin 9 (GAL9)

Gene Names

LGALS9; HUAT; LGALS9A

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>90%

Pricing

FAM-FLICA Caspase 1, Assay Kit (Cat# AAA12391)

Full Name

FAM-FLICA Caspase 1 Assay Kit

Applications

Flow Cytometry, Fluorescence Microscopy

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Application Data (Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha is included as a loading control. Detection is with Goat anti Rat IgG:Dylight®)

Application Data (HeLa cells stained with Rat anti tubulin apha green, nuclei are counterstained with DAPI)

Application Data (Published customer image:Stages of Mitosis in Naegleria Revealed by Staining for a-Tubulin, BN46/51 and DAPI. Images were obtained by conventional epifluorescence microscopy. A. Interphase; B. Prophase; C. Metaphase; D. Anaphase; E. Telophase. Bar = 10 um. Red, BN46/51; Green, a-tubulin; Blue, DAPI.Walsh CJ (2012) The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria. PLoS ONE 7(4): e34763.)

Application Data (Published customer image:Analysis of the Co-localization of Tubulin and the Nucleolar Protein BN46/51 During Prophase. Single 0.25 um optical sections at 1.0 um intervals are presented for two different cells. Columns A and D; raw data. Columns B and E; tubulin pixels are only displayed if the corresponding nucleolar protein pixel equaled or exceeded 25% of the maximum possible intensity. Columns C and F; tubulin pixels are only displayed if the corresponding nucleolar protein pixel equaled or exceeded 50% of maximum possible intensity. Numerical values are the summed fraction of tubulin intensity remaining in a given section after the subtraction. Red, BN 46/51; Green, a-tubulin. Bar = 10 um.From: Walsh CJ (2012) The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria. PLoS ONE 7(4): e34763.)

Application Data (Published customer image:T566 phosphorylation affects Bub1 stability. (A) BUB1-T566A mutant cells do not show substantial delay in recovering from nocodazole arrest. Wild-type (WT) and BUB1-T566A mutant cells were incubated with nocodazole (15 ug/mL) at 30 degree C for 90 min and then released from nocodazole arrest; at the indicated times, samples were taken to measure rebudded cells. (B) BUB1-T566A mutant cells show no significant sensitivity to nocodazole in a survival assay. Wild-type (WT), bub1?, and BUB1-T566A cells were incubated with nocodazole (15 ug/mL); at the indicated times, cells were washed out and approximately 200 cells were plated on a YPD plate. Cell viability was calculated by dividing the number of colonies formed at the 2.5 and 5 h time points by that formed in the absence of nocodazole (0 h) at 30 degree C. (C) Phosphorylation of T566 is important for degradation of Bub1 during anaphase but not during G1. cdc15-2 cells with HA-tagged Bub1 or Bub1-T566A expressed by the GAL1 promoter (GAL-BUB1 and GAL-BUB1-T566A) were arrested in anaphase (cdc15-2) or in G1 (alpha-factor) and then transferred to medium containing galactose for 2 h to induce Bub1 expression. Glucose was added to shut-off Bub1 expression; samples were taken at the indicated times for Western blot analyses with antibody to the HA epitope. Tubulin was used as a loading control. (D) The Bub1-T566A protein is more stable than wild-type in the presence of nocodazole. Wild-type and BUB1-T566A mutant cells (Bub1 and Bub1-T566A are tagged with myc) were incubated in the presence of nocodazole (10 ug/mL) at 30 degree C; at the indicated times, samples were taken for Western blot analyses with antibody to the myc epitope. Tubulin was used as a loading control.From: Goto GH, Mishra A, Abdulle R, Slaughter CA, Kitagawa K (2011) Bub1-Mediated Adaptation of the Spindle Checkpoint. PLoS Genet 7(1): e1001282.)

Application Data (Published customer image: Role of the phosphorylation of ATF7 in G2/M progression. (A) Parental HeLa S3/TR, HeLa S3/TR/ATF7-wt (cl.2), or HeLa S3/TR/ATF7-TA (cl.1) cells were synchronized using DTB and released into thymidine-free medium containing 1 ug/ml Dox for 12?h. Whole cell lysates were analyzed by WB. Full-length blots are presented in S12A Fig. (B) HeLa S3/TR/ATF7-wt (cl.2) (upper panels) or HeLa S3/TR/ATF7-TA (cl.1) cells (lower panels) were synchronized using single-thymidine block and released into thymidine-free medium containing 1 ug/ml Dox for 11 h. Cells were triply stained with anti-ATF7[SAB2500131] and anti-a-tubulin antibodies and PI (for DNA). Scale bars, 20 um. (C, D) Cells were stained with anti-histone H3pS10 antibody (for M phase) and PI for analyzing cell-cycle progression by flow cytometry. (C) Parental HeLa S3/TR, HeLa S3/TR/ATF7-wt (cl.2), or HeLa S3/TR/ATF7-TA (cl.1) cells were synchronized using DTB and released into thymidine-free medium containing 1 ug/ml Dox for 10.5~12.5 h. Two-dimensional histograms (DNA vs histone H3pS10) are presented together with DNA histograms, and the percentages of cells in G1 and M phases were measured. Cells in M and and G1 phases were quantitated from the results of S4 Fig. Values are means +/- SD (three independent clones). Asterisks indicate the significant difference (*P)

Application Data (Published customer image:Chromosomes remain associated into anaphase I of Cap-H2 mutants. Metaphase I and anaphase I morphologies were compared between wild-type and Cap-H2Z3-0019/Cap-H2TH1 mutant males. Testes were stained with DAPI and an anti-tubulin antibody to visualize DNA (white) and microtubules (green), respectively (scale bar in 3A indicates 10 um and 5 um in 3G). (A) Metaphase I in the wild-type. Each bivalent has congressed to the metaphase plate and appears as a cluster of DAPI stained material. (B) Anaphase I in the wild-type (DAPI only). Homologous chromosomes have segregated to daughter cells. (C) Anaphase I in the wild-type (DAPI and Tubulin merge). (D) Metaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male appears wild-type. (E) Anaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male (DAPI only). Chromatin bridges can be seen in three different segregation events. (F) Anaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant male (DAPI and Tubulin merge). (G) Higher resolution wild-type anaphase I image showing complete segregation of homologs. (H) Anaphase I from a Cap-H2Z3-0019/Cap-H2TH1 mutant demonstrating extensive chromatin bridging due to persistent associations between chromosomes migrating to opposing poles. (I) Anaphase I bridge found from a Cap-D3EY00456 mutant.From: Hartl TA, Sweeney SJ, Knepler PJ, Bosco G (2008) Condensin II Resolves Chromosomal Associations to Enable Anaphase I Segregation in Drosophila Male Meiosis. PLoS Genet 4(10): e1000228.)

Application Data (Published customer image:Cap-H2 mutants are defective in anaphase II segregation. Metaphase II and anaphase II morphologies were compared between wild-type and Cap-H2Z3-0019/Cap-H2TH1 mutant males. Testes were stained with DAPI and an anti-tubulin antibody to visualize DNA (white) and microtubules (green), respectively (scale bar in 6A indicates 10 um). (A) Wild-type metaphase/anaphase II cyst. Metaphase II cells are those where each bivalent has congressed to the metaphase plate and appear as a cluster of DAPI staining material. Anaphase II are those cells with two DAPI staining white clusters, indicating homologous chromosome segregation. (B) Metaphase II and anaphase II figures from a Cap-H2 strong mutant. Arrow indicates an anaphase II bridge. The arrowhead highlights an anaphase II bridge or lagging chromosome.From: Hartl TA, Sweeney SJ, Knepler PJ, Bosco G (2008) Condensin II Resolves Chromosomal Associations to Enable Anaphase I Segregation in Drosophila Male Meiosis. PLoS Genet 4(10): e1000228.)

Application Data (Published customer image:Confocal Microscopy of Mitotic Cells Stained for a-Tubulin and the Nucleolar Protein BN46/51. Five examples of progressively later stages of each mitotic stage are presented. Each image is a Z-axis projection of 0.25 um optical sections through an individual cell. The cell periphery is outlined in white. A. Interphase; B. Prophase; C. Metaphase; D. Anaphase; E. Telophase. F. Selected nuclei of, from left to right, Prophase, Metaphase, Anaphase, and Telophase. Bar = 10 um. Red, BN 46/51; Green, a-tubulin.From: Walsh CJ (2012) The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria. PLoS ONE 7(4): e34763.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12010)

Full Name

RAT ANTI TUBULIN ALPHA

Applications

Immunohistochemistry, Immunofluorescence, Radioimmunoassay, Western Blot

Pricing

Application Data (Fig-1: Induction of MDA5 activity by poly(I: C) prepacked with lipofectamine in MDA5 Leeporter HEK293T cells.)

MDA5/NF-kBT, Cell Line (Cat# AAA14874)

Full Name

MDA5/NF-kB Leeporter Luciferase Reporter-HEK293T Cell Line

Applications

Functional Assay

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Application Data (Fig-5: Cell Surface staining of human ACE2 in the ACE2/HEK293 stable cell line. ATTO 488-conjugated anti-hACE2 antibody (clone AC18F; was used at 1ug/ 1x10^6 cells (Red). ATTO 488-conjugated mouse IgG1 was used as isotype control at 1ug/ 1x10^6 cells (Green).)

Application Data (Fig-4: Genomic PCR analysis. Genomic DNAs were purified from ACE2/HEK293 stable cells and parental HEK293 cells, and used as the PCR templates. PCR was performed using the target gene construct-specific primer sets, and the plasmid DNA of target gene construct was used as positive control.)

Application Data (Fig-3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.)

Application Data (Fig-2: Detection of ACE2 expression in ACE2/HEK293 cells by flow cytometry. ACE2/HEK293 cells were probed with biotinylated SARS-Cov-2 Spike RBD protein at 1 x 10^5 cells per 1 ug protein on ice for 2 hours. Cells were washed with flow buffer and then incubated with PE-labeled Streptavidin on ice for 20 min. Cell were washed and then analyzed by flow cytometry.)

Application Data (Fig-1: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein and analyzed through In-Cell ELISA using HRP-Streptavidin for detection.)

ACE2, Cell Line (Cat# AAA14888)

Full Name

ACE2/HEK293 Stable Cell Line

Gene Names

ACE2; ACEH

Applications

Functional Assay

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5 results for "Cell Viability Assay" - showing 1-5

Galectin 9 (GAL9), Active Protein (Cat# AAA21103)

FAM-FLICA Caspase 1, Assay Kit (Cat# AAA12391)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12010)

MDA5/NF-kBT, Cell Line (Cat# AAA14874)

ACE2, Cell Line (Cat# AAA14888)

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5 results for "Cell Viability Assay" - showing 1-5

Galectin 9 (GAL9), Active Protein (Cat# AAA21103)

FAM-FLICA Caspase 1, Assay Kit (Cat# AAA12391)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12010)

MDA5/NF-kBT, Cell Line (Cat# AAA14874)

ACE2, Cell Line (Cat# AAA14888)

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