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7 results for " notch" - showing 1-7
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using NOTCH1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
NOTCH1, Polyclonal Antibody (Cat# AAA22197)
Full Name
NOTCH1 Polyclonal Antibody
Gene Names
NOTCH1; hN1; TAN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Notch 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Notch 1, Monoclonal Antibody (Cat# AAA30050)
Full Name
Notch 1 Antibody
Gene Names
NOTCH1; hN1; TAN1
Reactivity
Human, Mouse
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using ITCH antibody at dilution of 1:100 (40x lens).)
ITCH, Polyclonal Antibody (Cat# AAA28315)
Full Name
[KO Validated] ITCH Rabbit pAb
Gene Names
ITCH; AIF4; AIP4; NAPP1; dJ468O1.1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(T98G cells were stained with AAA14690 (filled histogram) or isotype control (open histogram).)
Delta-like 1, Monoclonal Antibody (Cat# AAA14690)
Full Name
Mouse Anti-Delta-like 1
Reactivity
Human
Applications
FC
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
IP (Immunoprecipitation)
(HDAC2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate4 uL anti-HDAC2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HDAC2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)
HDAC2, Polyclonal Antibody (Cat# AAA27718)
Full Name
Anti-HDAC2 Antibody, Rabbit Polyclonal
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)
Full Name
Anti-Musashi 1/Msi1 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with (1:25), followed by Dylight 488- conjugated goat anti- rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OScell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1:100) (red).)
DLL3, Polyclonal Antibody (Cat# AAA26850)
Full Name
DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)
Gene Names
DLL3; SCDO1
Reactivity
Human
Applications
IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing