Search results for " Vascular cell adhesion molecule"

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WB (Western Blot) (Western Blot Analysis of human Jurkat cell lysate using CD31 Mouse Monoclonal Antibody (C31.7).)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13801)

Full Name

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human, Cynomolgus Monkey, Rabbit.
Weakly reacts with Rat

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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Application Data (Published Customer Image:Mouse CD31 antibody, clone ER-MP12 used for the demonstration of vasculature in mouse brain by immunofluorescence.Image caption:Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n?=?5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean?+/-SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p?)

CD31, Monoclonal Antibody (Cat# AAA12258)

Full Name

Rat Anti Mouse CD31: FITC

Gene Names

Pecam1; Cd31; Pecam; C85791; PECAM-1

Reactivity

Mouse

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

Soluble Vascuolar Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA17490)

Full Name

Rat Soluble Vascuolar Cell Adhesion Molecule 1 ELISA Kit

Reactivity

Rat

Pricing

vascular cell adhesion protein 1 (VCAM-1/CD106), ELISA Kit (Cat# AAA15402)

Full Name

Rat vascular cell adhesion protein 1, VCAM-1/CD106 ELISA Kit

Gene Names

Vcam1; VCAM1B

Reactivity

Rat

Pricing

Vascular Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA22583)

Full Name

Canine Vascular Cell Adhesion Molecule 1 (VCAM-1) ELISA Kit

Gene Names

VCAM1; CD106; INCAM-100

Reactivity

Canine

Pricing

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Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for binding efficiency determinationImage caption:Binding efficiencies (BE) of different a4beta7 molecules composed of distinct a4 mutants to monoclonal antibodies against a4, beta7 or the a4beta7 heterodimer. Binding efficiency is determined by the ratio between the mean fluorescence of antibody binding to each a4 molecule and of the binding in a mock-transfected cell culture (see Materials and Methods for details). Dark gray bars represent binding to the human (wild type) a4 clone, whereas light gray bars are those of binding to the different a4 mutants (as shown in the x-axis). a4 mutants which included substitutions at codon 201 are boxed. A, binding of anti-a4 2b4 antibody. B, binding of anti-a4 HP2/1 antibody. C, BE of different anti-a4 and beta7 antibodies to the human a4 and the quintuple a4 mutant (5 aa mut). Bars represent the range of standard errors deduced from triplicate experiments. p-values of Student's t tests are shown above each comparison. NS, non-significant (> 0.05).From:Darc M, Hait SH, Soares EA, Cicala C, Seuanez HN, et al. (2011) Polymorphisms in the a4 Integrin of Neotropical Primates: Insights for Binding of Natural Ligands and HIV-1 gp120 to the Human a4beta7. PLoS ONE 6(9): e24461.)

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for western blotting and immunoprecipitationImage caption:Tetraspanins CD81, CD82 and CD151 are associated with a4beta1 throughout erythroid maturation and with beta3 in proerythroblasts and basophilic erythroblasts. A. CD81, CD82 and CD151 precipitates from Mn2+-activated proerythroblasts (ProEB, day 5), basophilic (BasoEB, day 8) and polychromatic (PolyEB, day 12) erythroblasts were successively probed with anti-a4, anti-beta1 and anti-beta3 antibodies; tetraspanin controls from each time point are also illustrated. All tetraspanins co-precipitated a4 and beta1 from erythroblasts B. Tetraspanin precipitates from day 6 proerythroblasts (ProEB) solubilised in the presence of EDTA or different cations, and from Mn2+-activated basophilic erythroblasts (BasoEB, day 8) were probed with a mix of antibodies to a5, beta1, beta2 and beta3 integrins while the control samples were probed with the relevant tetraspanin antibodies. For clarity, integrin controls are illustrated for the EDTA blot but were present on all blots. beta1 and beta3 integrins were precipitated well only in the presence of Mn2+. C. CD81 and CD82 precipitates from day 5 proerythroblasts were successively probed with different anti-integrin subunit antibodies and demonstrate co-precipitation of beta1 and beta3 but not a5 or beta2 integrins. D. CD81 (454720) and CD82 (53H5) precipitates from day 6 proerythroblasts (ProEB) and HEL cells (HEL) solubilised in the presence of EDTA, Ca2++Mg2+ or Mn2+ probed with anti-CD82 and anti-CD81 antibodies. Each tetraspanin co-precipitates the other most strongly in the presence of Mn2+ from proerythroblasts while any cation permits co-precipitation in HEL cells. Integrins were analysed on 7.5% gels, tetraspanins on 12% gels; non-reducing conditions. Unless stated, the following clones were used: CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; a5, IIA1; aL, TS1/22; aIIb, PAB-1. All day 5 and 6 cultures comprised 90 -95% proerythroblasts; day 8 culture comprised 5% proerythroblasts, 81% basophilic erythroblasts and 14% polychromatic erythroblasts; day 12 culture comprised 41% polychromatic erythroblasts, 15% orthochromatic erythroblasts and 41% reticulocytes. In the day 5 and 6 cultures 15 -34% of cells were GPA+ and 28 -35% of cells were aIIb+. Day 8 and day 12 cultures had 77% and 97% GPA+ cells, respectively, and 9% and 0% aIIb+ cells, respectively.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4beta1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1. PLoS ONE 8(5): e62654.)

CD49d, Monoclonal Antibody (Cat# AAA12003)

Full Name

MOUSE ANTI HUMAN CD49d

Gene Names

ITGA4; IA4; CD49D

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

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Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for binding efficiency determinationImage caption:Binding efficiencies (BE) of different a4beta7 molecules composed of distinct a4 mutants to monoclonal antibodies against a4, beta7 or the a4beta7 heterodimer. Binding efficiency is determined by the ratio between the mean fluorescence of antibody binding to each a4 molecule and of the binding in a mock-transfected cell culture (see Materials and Methods for details). Dark gray bars represent binding to the human (wild type) a4 clone, whereas light gray bars are those of binding to the different a4 mutants (as shown in the x-axis). a4 mutants which included substitutions at codon 201 are boxed. A, binding of anti-a4 2b4 antibody. B, binding of anti-a4 HP2/1 antibody. C, BE of different anti-a4 and beta7 antibodies to the human a4 and the quintuple a4 mutant (5 aa mut). Bars represent the range of standard errors deduced from triplicate experiments. p-values of Student's t tests are shown above each comparison. NS, non-significant (> 0.05).From:Darc M, Hait SH, Soares EA, Cicala C, Seuanez HN, et al. (2011) Polymorphisms in the a4 Integrin of Neotropical Primates: Insights for Binding of Natural Ligands and HIV-1 gp120 to the Human a4beta7. PLoS ONE 6(9): e24461.)

Application Data (Published customer image: Mouse anti Human CD49d antibody, clone HP2/1 used for western blotting and immunoprecipitationImage caption:Tetraspanins CD81, CD82 and CD151 are associated with a4beta1 throughout erythroid maturation and with beta3 in proerythroblasts and basophilic erythroblasts. A. CD81, CD82 and CD151 precipitates from Mn2+-activated proerythroblasts (ProEB, day 5), basophilic (BasoEB, day 8) and polychromatic (PolyEB, day 12) erythroblasts were successively probed with anti-a4, anti-beta1 and anti-beta3 antibodies; tetraspanin controls from each time point are also illustrated. All tetraspanins co-precipitated a4 and beta1 from erythroblasts B. Tetraspanin precipitates from day 6 proerythroblasts (ProEB) solubilised in the presence of EDTA or different cations, and from Mn2+-activated basophilic erythroblasts (BasoEB, day 8) were probed with a mix of antibodies to a5, beta1, beta2 and beta3 integrins while the control samples were probed with the relevant tetraspanin antibodies. For clarity, integrin controls are illustrated for the EDTA blot but were present on all blots. beta1 and beta3 integrins were precipitated well only in the presence of Mn2+. C. CD81 and CD82 precipitates from day 5 proerythroblasts were successively probed with different anti-integrin subunit antibodies and demonstrate co-precipitation of beta1 and beta3 but not a5 or beta2 integrins. D. CD81 (454720) and CD82 (53H5) precipitates from day 6 proerythroblasts (ProEB) and HEL cells (HEL) solubilised in the presence of EDTA, Ca2++Mg2+ or Mn2+ probed with anti-CD82 and anti-CD81 antibodies. Each tetraspanin co-precipitates the other most strongly in the presence of Mn2+ from proerythroblasts while any cation permits co-precipitation in HEL cells. Integrins were analysed on 7.5% gels, tetraspanins on 12% gels; non-reducing conditions. Unless stated, the following clones were used: CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; a5, IIA1; aL, TS1/22; aIIb, PAB-1. All day 5 and 6 cultures comprised 90 -95% proerythroblasts; day 8 culture comprised 5% proerythroblasts, 81% basophilic erythroblasts and 14% polychromatic erythroblasts; day 12 culture comprised 41% polychromatic erythroblasts, 15% orthochromatic erythroblasts and 41% reticulocytes. In the day 5 and 6 cultures 15 -34% of cells were GPA+ and 28 -35% of cells were aIIb+. Day 8 and day 12 cultures had 77% and 97% GPA+ cells, respectively, and 9% and 0% aIIb+ cells, respectively.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, et al. (2013) Tetraspanins CD81 and CD82 Facilitate a4beta1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1. PLoS ONE 8(5): e62654.)

CD49d, Monoclonal Antibody (Cat# AAA12002)

Full Name

MOUSE ANTI HUMAN CD49d

Gene Names

ITGA4; IA4; CD49D

Reactivity

Bovine, Cat, Cynomolgus monkey, Goat, Horse, Llama, Mink, Mustelid, Pig, Rabbit, Rat, Rhesus Monkey

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

VCAM-1, ELISA Kit (Cat# AAA21486)

Full Name

Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit

Gene Names

Vcam1; VCAM1B

Reactivity

Rat

Pricing

Vascular Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA16858)

Full Name

Porcine Vascular Cell Adhesion Molecule 1 ELISA Kit

Gene Names

Vcam1; VCAM1B

Reactivity

Porcine

Pricing

Mucosal addressin cell adhesion molecule 1 (MADCAM1), Recombinant Protein (Cat# AAA18733)

Full Name

Recombinant Human Mucosal addressin cell adhesion molecule 1 Recombinant Human Mucosal addressin cell adhesion molecule 1 (MADCAM1), partial

Gene Names

MADCAM1; MACAM1

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Pricing

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FCM (Flow Cytometry) (Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD31 Mouse Monoclonal Antibody (JC/70A) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13818)

Full Name

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human, Cynomolgus Monkey, Rabbit.
Does not react with Rat and Pig

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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11 results for " Vascular cell adhesion molecule" - showing 1-11

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13801)

CD31, Monoclonal Antibody (Cat# AAA12258)

Soluble Vascuolar Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA17490)

vascular cell adhesion protein 1 (VCAM-1/CD106), ELISA Kit (Cat# AAA15402)

Vascular Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA22583)

CD49d, Monoclonal Antibody (Cat# AAA12003)

CD49d, Monoclonal Antibody (Cat# AAA12002)

VCAM-1, ELISA Kit (Cat# AAA21486)

Vascular Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA16858)

Mucosal addressin cell adhesion molecule 1 (MADCAM1), Recombinant Protein (Cat# AAA18733)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13818)

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11 results for " Vascular cell adhesion molecule" - showing 1-11

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13801)

CD31, Monoclonal Antibody (Cat# AAA12258)

Soluble Vascuolar Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA17490)

vascular cell adhesion protein 1 (VCAM-1/CD106), ELISA Kit (Cat# AAA15402)

Vascular Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA22583)

CD49d, Monoclonal Antibody (Cat# AAA12003)

CD49d, Monoclonal Antibody (Cat# AAA12002)

VCAM-1, ELISA Kit (Cat# AAA21486)

Vascular Cell Adhesion Molecule 1, ELISA Kit (Cat# AAA16858)

Mucosal addressin cell adhesion molecule 1 (MADCAM1), Recombinant Protein (Cat# AAA18733)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13818)

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