Search results for " Rabbit IgGs for Coronavirus"

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3 results for " Rabbit IgGs for Coronavirus" - showing 1-3

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WB (Western Blot) (Figure 12 Overexpression Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike, AAA10931 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells.)

IHC (Immunohistochemistry) (Figure 11 IHC Validation of SARS-CoV2 Spike in COVID-19 Patient Brain Spike protein was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the brain of the COVID-19 patient confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IHC (Immunohistochemistry) (Figure 10 IHC Validation of SARS-CoV2 Spike in COVID-19 Patient Lung Strong spike signal was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the lung of the COVID-19 patient confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IHC (Immunohistchemistry) (Figure 9 IHC Validation of SARS-CoV2 Spike in the Nasopharyngeal Swab Sample of the COVID-19 Patient Strong spike signal was detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) in the nasopharyngeal swab sample of the COVID-19 patient and no spike signal was observed in the sample of the COVID-19 negative patient. COVID-19 cases were confirmed by PCR. (Courtesy of Dr. Nuovo Gerard J., OSU))

IF (Immunofluorescence) (Figure 8 IF Validation of SARS-CoV2 Spike in COVID-19 Patient Skin (Magro et al., 2020) C4d is highlighted green while COVID-19 spike protein detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) shows a red staining pattern; a yellow signal is discernible indicative of co-localization of C4d and viral protein within the microvasculature.)

IF (Immunofluorescence) (Figure 7 IF Validation of SARS-CoV2 Spike in COVID-19 Patient Lung (Magro et al., 2020) SARS-CoV2 spike protein (red, panel C) detected by anti-spike antibodies (AAA10931, 0.2 ug/mL) colocalized with C4d (green in panel d, merged in yellow). Spike protein (red, panel g) was also colocalized with C5b-9 (green in panel f&h, merged in yellow))

IHC (Immunohistchemistry) (Figure 6 IHC/IF Validation in COVID-19 Patient Sample (Nuovo et al., 2020) Detection of SARS-CoV-2 proteins in nasopharyngeal swab cell preparations. B. An intense signal for covid-19 spike protein tested by SARS-CoV-2 spike antibodies (AAA10931) was observed in the glandular cells. F-H. Co-expression of spike detected by spike antibodies (AAA10931, 0.2 ug/mL) and envelope proteins detected by envelope antibodies (, 2 ug/mL) of SARSCoV- 2 (F) documented localization of each protein to glandular cells (G, yellow). No signal was seen in oral swabs of positive cases (H). Both the spike and envelope protein detected by anti-spike antibodies (AAA10931) and anti-envelope antibodies (,) produced a signal in the nasopharyngeal swabs of the three cases and no signal was evident in the nasopharyngeal swabs of the seven controls.)

IHC (Immunohistochemistry) (Figure 5 Immunohistochemistry Validation of SARSCoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA10931, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal wasobserved in macrophages of COVID-19 patient lung.)

IHC (Immunohistochemistry) (Figure 4 Immunohistochemistry Validation of SARSCoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (AAA10931, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung, but not in non-COVID-19 patient lung.)

IF (Immunofluorescence) (Figure 3 Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021) Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, AAA10931. (k-s) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k-m); pan-macrophage marker CD68 (magenta) (n-p); HLA-DR (magenta) and pDC marker CD123 (yellow) (q–s) and DAPI (blue).)

IF (Immunofluorescence) (Figure 2 Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Nose and Tonsil (Singh et al., Nature Microbiology, 2021) Multi-label confocal immunofluorescence microscopy of nasal epithelium (20X-b, 63xh) and tonsil (20X-c,63X-i) from rhesus macaques infected with SARS-CoV-2 with SARS-CoV-2 spike-specific antibodies, AAA10931. (turquoise), DAPI (blue). Rabbit IgG isotype control antibody was used to stain the tissues to rule out any nonspecific staining (e, f).)

IF (Immunofluorescence) (Firure 1. Immunofluorescent Validation of AAA10931 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021)Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, AAA10931. (k, n) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k); pan-macrophage marker CD68 (magenta) (n) and DAPI (blue).)

COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA10931)

Full Name

SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody

Reactivity

Virus

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistochemistry) (IHC Validation of Envelope in COVID-19 Patient Skin (Magro et al., 2020) Detection of SARS-CoV-2 Envelope protein in the blood vessels of COVID-19 patients that were confirmed by PCR. The staining shows Envelope protein expression (green) detected by envelope antibodies (AAA10932, 3 ug/mL) in the endothelial cytoplasms in thrombosed and normal appearing blood vessels with hematoxylin counterstain. The staining was negative in control normal skin/lung (not shown).)

IF (Immunofluorescence) (Co-expression of SARS-CoV-2 (COVID-19) Envelope and C5b-9 in Human Lung Tissue from the COVID-19 Patient Immunofluorescent l analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Envelope antibody (AAA10932, 2 ug/mL, red) and anti-C5b-9 antibody (green). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C, followed by secondary antibodies at 1/500 and DAPI staining (blue). Co- expression was shown in yellow. (Courtesy of Dr. Nuovo Gerard J., OSU).)

IF (Immunofluorescence) (Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Envelope in Human Lung Tissue from the COVID-19 Patient Immunofluoorescent l analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Envelope antibody (AAA10932, 2 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C, followed by a goat anti-rabbit IgG secondary antibody at 1/500 (red) and DAPI staining (blue). (Courtesy of Dr. Nuovo Gerard J., OSU).)

IF (Immunofluorescence) (IF Validation of Envelope in COVID-19 Patient Skin (Magro et al., 2020) Detection of SARS-CoV-2 Envelope protein in the skin of COVID-19 patients that were confirmed by PCR. The skin staining shows Envelope protein expression (green) detected by envelope antibodies (AAA10932, 3 ug/mL) in mononuclear cells with hematoxylin counterstain. The staining was negative in control normal skin/lung (not shown).)

IHC (Immunohistochemistry) (Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Envelope in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Envelope antibody (AAA10932, 1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 envelope protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)

ELISA (Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody, (AAA10932) (1 ug/mL). A direct ELISA was performed using antigen or control peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 32 ng/mL to 2000ng/mL.)

IHC (Immunohistochemistry) (IHC Validation in COVID-19 Patient Sample. Detection of SARS-CoV-2 Envelope protein in nasopharyngeal swab samples of COVID-19 patients Panel F shows Envelope protein detected by envelope antibodies (AAA10932) was still evident 2 weeks after the initial swabs (signal is red with hematoxylin counterstain), though the amount of virus was much less than at the initial swab.)

IHC (Immunohistochemistry) (Validation in COVID-19 Patient Sample. Detection of SARS-CoV-2 proteins in nasopharyngeal swab cell preparations F-H. Co-expression of spike detected by spike antibodies and envelope proteins detected by envelope antibodies (AAA10932) of SARS-CoV-2 (panel F) documented localization of each protein to glandular cells with negative squamous cells two weeks after full revoery (panel G, signal yellow). No signal was seen in oral swabs of positive cases (Panel H). Both the spike and envelope protein detected by anti-spike antibodies and anti-envelope antibodies (AAA10932) produced a signal in the nasopharyngeal swabs of the three vases and no signal was evident in the nasopharyngeal swabs of the seven controls.)

COVID 19 Envelope Coronavirus, Polyclonal Antibody (Cat# AAA10932)

Full Name

SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody

Reactivity

Virus

Applications

Immunofluorescence, Immunohistochemistry

Purity

SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody is affinity chromatography purified via peptide column.

Pricing

Application Data

COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA13774)

Full Name

Anti-Spike Protein (SARS-CoV-2) Polyclonal Antibody

Applications

Western Blot

Purity

Protein G immunoaffinity chromatography

Pricing

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3 results for " Rabbit IgGs for Coronavirus" - showing 1-3

COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA10931)

COVID 19 Envelope Coronavirus, Polyclonal Antibody (Cat# AAA10932)

COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA13774)

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