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Application
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296 results for " Purified Secondary Antibodies" - showing 1-50
WB (Western Blot)
(WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)
PSEN1, Polyclonal Antibody (Cat# AAA23585)
Full Name
PSEN1 antibody - middle region
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Affinity Purified
Pricing
WB (Western Blot)
(FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)
FGFR2, Polyclonal Antibody (Cat# AAA28728)
Full Name
FGFR2 Antibody (N-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, mouse, rat, monkey
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IP (Immunoprecipitation)
(Figure 11 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10944)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26902)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26762)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (HRP)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
Application Data
(S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)
S100A8, Monoclonal Antibody (Cat# AAA14670)
Full Name
S100A8 (MRP-8)
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
Application Data
(Figure 10 KD Validation in HeLa cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 ?M luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies (1139). DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10952)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Purity
DR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26899)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody (Center). AAA28746 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
HIST1H2AG, Polyclonal Antibody (Cat# AAA28746)
Full Name
HIST1H2AG Antibody (Center)
Gene Names
HIST1H2AI; H2A/c; H2AFC
Reactivity
Human (Predicted Reactivity: Rat, Mouse, Bovine, Xenopus, Yeast, Chicken, Monkey, Zebrafish, Drosophila)
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with CD99 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CD99, Monoclonal Antibody (Cat# AAA30274)
Full Name
CD99 Antibody
Gene Names
CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
GAPDH, Polyclonal Antibody (Cat# AAA28693)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center K159), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
PDIA6, Polyclonal Antibody (Cat# AAA28792)
Full Name
PDIA6 Antibody (Center K159)
Gene Names
PDIA6; P5; ERP5; TXNDC7
Reactivity
Human, mouse, rat
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26896)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26895)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IHC (Immunohistchemistry)
(Validation of PKR in Rat Lung Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (AAA10917) at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
PKR, Polyclonal Antibody (Cat# AAA10917)
Full Name
PKR Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PKR Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26894)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IHC (Immunohistochemistry)
(ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ATP5B, Polyclonal Antibody (Cat# AAA28724)
Full Name
ATP5B Antibody (Center)
Gene Names
ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Figure 10 Immunofluorescence images of PUMA in the P14 rat retina (Wakabayashi et al., 2012)PUMA expression in the rat retina detected by anti-PUMA antibodies (3043). The specimens were counterstained with Hoechst 33258 to visualize nuclei (+DNA). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; P, postnatal day.)
PUMA, Polyclonal Antibody (Cat# AAA10934)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26737)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (Biotin)
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
WB (Western Blot)
(EREG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EREG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
EREG, Polyclonal Antibody (Cat# AAA28771)
Full Name
EREG Antibody (C-term)
Gene Names
EREG; ER
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of lysates from Hela, HepG2, MCF-7, SH-SY5Y, mouse NIH/3T3 cell line (from left to right), using FGFR1 Antibody (Center). AAA28687 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
FGFR1, Polyclonal Antibody (Cat# AAA28687)
Full Name
FGFR1 Antibody (Center)
Gene Names
FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Rat)
Applications
Flow Cytometry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SOCS1, Polyclonal Antibody (Cat# AAA28752)
Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
ICC (Immunocytochemistry)
(Figure 10 Immunocytochemistry Validation of PD-L1Immunocytochemical analysis of 4% paraformaldehyde-fixed PD-L1 transfected 293 cells labeling PD-L1 with at 1 μg/ml, followed by Goat anti-mouse IgG secondary antibody at 1/250 dilution (red). Lower left: Use mouse IgG antibody at 1 μg/ml as control.)
PDL1, Monoclonal Antibody (Cat# AAA10992)
Full Name
PDL1 Antibody [6H10]
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A purified
Pricing
FCM (Flow Cytometry)
(HAS2 Antibody (Center) (AAA28714) flow cytometric analysis of K562 cells (bottom histogram) compared to a Rabbit IgG Isotype control (negative control-top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
HAS2, Polyclonal Antibody (Cat# AAA28714)
Full Name
HAS2 Antibody (Center)
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of TMEM41B in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
TMEM41B, Polyclonal Antibody (Cat# AAA11035)
Full Name
TMEM41B (CT) Antibody
Reactivity
Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
Applications
Immunofluorescence, Western Blot
Purity
TMEM41B Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Vimentin, Polyclonal Antibody (Cat# AAA28770)
Full Name
Vimentin Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Anti-SOST Antibody (N-term)at 1:2000 dilution + human kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SOST, Polyclonal Antibody (Cat# AAA28704)
Full Name
SOST Antibody (N-term)
Gene Names
SOST; CDD; VBCH; SOST1
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(IHC staining of immersion-fixed frozen section of mouse embryo (13 d.p.c.) using AAA14708 at 1.7ug/ml, with overnight incubation at 4°C. HRP-conjugated anti-goat secondary antibody and DAB staining were used for visualization of Reelin which in this sample was localized to the developing brain. The tissue section was counterstained with hematoxylin (blue).)
Reelin, Polyclonal Antibody (Cat# AAA14708)
Full Name
Reelin
Gene Names
RELN; RL; LIS2; PRO1598
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Pricing
FCM (Flow Cytometry)
(CDK4 Antibody (C-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CDK4, Polyclonal Antibody (Cat# AAA28747)
Full Name
CDK4 Antibody (C-term)
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110β (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110β (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA14720)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)
S100B, Polyclonal Antibody (Cat# AAA28759)
Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat (Predicted: Rabbit)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(CYK18 Antibody (C-term) Western blot of A431 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow) (Kindly offered by Dr. Li).)
KRT18 / CK18 / Cytokeratin 18, Polyclonal Antibody (Cat# AAA12370)
Full Name
Rabbit Polyclonal to Human KRT18 / CK18 / Cytokeratin 18
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Ammonium sulfate precipitation
Pricing
WB (Western Blot)
(Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.)
HLA-B, Polyclonal Antibody (Cat# AAA28743)
Full Name
HLA-B Antibody (N-term)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(PLD6 Antibody (Center) (Cat# AAA28684) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).)
PLD6, Polyclonal Antibody (Cat# AAA28684)
Full Name
PLD6 Antibody (Center)
Gene Names
PLD6; ZUC
Reactivity
Human, Mouse
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26897)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26901)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Figure 9 KD Validation in HeLa Cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 μM luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies. DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10950)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
Western Blot
Purity
DR4 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(Figure 5 Immunohistochemistry Validation of TIM-1Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
TIM-1, Polyclonal Antibody (Cat# AAA10935)
Full Name
TIM-1 Antibody
Gene Names
HAVCR1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
TIM-1 Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of NOS2A Antibody (Center) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
NOS2A, Polyclonal Antibody (Cat# AAA28780)
Full Name
NOS2A Antibody (Center)
Gene Names
NOS2; NOS; INOS; NOS2A; HEP-NOS
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26900)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of SRC Antibody (N-term) with A431 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
SRC, Polyclonal Antibody (Cat# AAA28721)
Full Name
SRC Antibody (N-term)
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
CD38, Polyclonal Antibody (Cat# AAA28702)
Full Name
CD38 Antibody (C-term)
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Figure 9 KD Validation of PUMA (Han et al., 2010)Immunoblot analyses of Tet-induced p53 cells treated with NOXA, Puma, Bim or non-targeting siRNAs that were utilized in this experiment. PUMA protein levels were markedly reduced in PUMA KD cells detected by anti-PUMA antibodies (3041).)
PUMA, Polyclonal Antibody (Cat# AAA10943)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CYK18 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
CYK18, Polyclonal Antibody (Cat# AAA28709)
Full Name
CYK18 Antibody (C-term)
Gene Names
KRT18; K18; CYK18
Reactivity
Human, mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating GAPDH in Hela whole cell lysateLane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (5ul) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug)For western blotting, the blot was detected at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000)
GAPDH, Monoclonal Antibody (Cat# AAA27042)
Full Name
GAPDH Monoclonal Antibody
Reactivity
Human, Mouse, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(CRFR2 Antibody (D35) flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CRHR2 / CRF2 Receptor, Polyclonal Antibody (Cat# AAA21272)
Full Name
Anti-CRHR2 / CRF2 Receptor Antibody (aa10-39) IHC-plus
Gene Names
CRHR2; CRF2; CRFR2; CRF-RB; HM-CRF
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Protein A purified
Pricing
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of TMEM41B in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
TMEM41B, Polyclonal Antibody (Cat# AAA11036)
Full Name
TMEM41B (NT) Antibody
Reactivity
Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Horse (100%); Chimpanzee (100%).
Predicted species reactivity based on immunogen sequence: Horse (100%); Chimpanzee (100%).
Applications
Immunofluorescence, Western Blot
Purity
TMEM41B Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26904)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
GAPDH, Polyclonal Antibody (Cat# AAA28776)
Full Name
GAPDH Antibody (N-term)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Strength of Signal
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Vimentin, Monoclonal Antibody (Cat# AAA13810)
Full Name
Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human, Cow, Dog, Cat, Pig, Goat, Chicken.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing