Search results for " Phosphoenolpyruvate Assay Kit"

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti- PCK2 antibody (AAA19384).Overlay histogram showing MCF-7 cells stained with AAA19384 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PCK2 Antibody (AAA19384, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of PCK2 using anti-PCK2 antibody (AAA19384).PCK2 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- PCK2 Antibody (AAA19384) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PCK2 using anti-PCK2 antibody (AAA19384).PCK2 was detected in paraffin-embedded section of human retcal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PCK2 Antibody (AAA19384) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PCK2 using anti-PCK2 antibody (AAA19384).PCK2 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PCK2 Antibody (AAA19384) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PCK2 using anti-PCK2 antibody (AAA19384).PCK2 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PCK2 Antibody (AAA19384) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PCK2 using anti-PCK2 antibody (AAA19384).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEPG2 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: human HEK293 whole cell lysatesLane 4: human A431 whole cell lysatesLane 5: monkey COS-7 whole cell lysatesLane 6: rat kidney tissue lysatesLane 7: mouse kidney tissue lysatesLane 8: mouse Neuro-2a whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-PCK2 antigen affinity purified monoclonal antibody (Catalog # AAA19384) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71KD. The expected band size forPCK2 is at 71KD.)

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2 results for " Phosphoenolpyruvate Assay Kit" - showing 1-2

Phosphoenolpyruvate Carboxylase, Assay Kit (Cat# AAA27842)

PCK2, Monoclonal Antibody (Cat# AAA19384)

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