Search results for " Other proteases"

3 results for " Other proteases" - showing 1-3

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WB (Western Blot) (Sample: Recombinant PRF1, Human;Antibody: Rabbit Anti-Human PRF1 Ab)

Activity (Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. Besides, Calreticulin (CRT) has been identified as an interactor of PRF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PRF1 and recombinant human CRT. Briefly, PRF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CRT-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PRF1 and CRT was shown in Figure 1, and this effect was in a dose dependent manner.The activity of recombinant PRF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant human PRF1 with 0.9% NaCl, add 50uL a serial dilution of PRF1, 10uL 0.1M CaCl2 to each well, then add 50uL 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 10uL 0.9% NaCl to reaplace CaCl2 in control wells. The plate is incubated for 20 hours at 37 degree C, 5% CO2. The results are shown in Figure 2. It was obvious that the minimal effective concentration of PRF1 is 12.5ug/mL.)

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

Full Name

Active Perforin 1 (PRF1)

Gene Names

PRF1; P1; PFP; HPLH2

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>80%

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of GALNS using anti-GALNS antibody (AAA19164).GALNS was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALNS Antibody (AAA19164) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GALNS, Antibody (Cat# AAA19164)

Full Name

Anti-GALNS Picoband antibody

Gene Names

GALNS; GAS; MPS4A; GalN6S; GALNAC6S

Reactivity

Reacts with: Human, Mouse, Rat

Applications

WB, EIA

Purity

Immunogen affinity purified

Pricing

TAT, Monoclonal Antibody (Cat# AAA17937)

Full Name

TAT antibody

Applications

Immunohistochemistry

Purity

TAT antibody was protein-A purified

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3 results for " Other proteases" - showing 1-3

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

GALNS, Antibody (Cat# AAA19164)

TAT, Monoclonal Antibody (Cat# AAA17937)

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3 results for " Other proteases" - showing 1-3

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

GALNS, Antibody (Cat# AAA19164)

TAT, Monoclonal Antibody (Cat# AAA17937)

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