Search results for " OA"

5 results for " OA" - showing 1-5

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Application Data (FASN antibody at 1/2000 dilution interacts with recombinant FASN protein with a DsbA tag. Predicted band size: 50Kda. Observed band size: 50Kda.)

FASN, Antibody (Cat# AAA17951)

Full Name

FASN Antibody

Gene Names

FASN; FAS; OA-519; SDR27X1

Reactivity

Human

Applications

Western Blot

Purity

Protein G-Sepharose affinity purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with Fatty Acid Synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Fatty Acid Synthase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Fatty Acid Synthase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.)

Fatty Acid Synthase, Monoclonal Antibody (Cat# AAA30258)

Full Name

Fatty Acid Synthase Antibody

Gene Names

FASN; FAS; OA-519; SDR27X1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

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Application Data

Application Data (Hela Cells were treated with BrdU for 3 hours and stained with Mouse anti BrdU antibody, clone Bu20a . Goat anti Mouse IgG (H/L) DyLight 549 conjugated antibody (red) was used as the secondary antibody. Cytoplasm was stained with Rabbit anti GAPDH antibody . As the secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody (green) was used. PureBlu DAPI (1351303) was used as nuclear counterstain (blue).)

Application Data (HeLa Cells were treated with 10 mug BrdU for 1 hour (B) or left untreated (A). Cells were stained with Mouse anti BrdU antibody, clone Bu20a at a dilution of 1/25. As a secondary antibody, Goat anti Mouse IgG (H/L) DyLight 549 conjugated antibody (red) was used at a 1/50 dilution. Cytoplasm was stained with Rabbit anti GAPDH antibody at a dilution of 1/100. As a secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody (green) was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.)

Application Data (Published Customer Image:Mouse Anti Bromodeoxyuridine antibody, clone Bu20a used to stain dividing crypt cells in rat small intestine by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:BrdU/ Bu20a/ Small intestine/ Rat.From: Furukawa S, Nagaike M, Ozaki K.Databases for technical aspects of immunohistochemistry.J Toxicol Pathol. 2017 Jan;30(1):79-107.)

Application Data (Published Customer image:Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.Image caption:Ghrelin does not significantly inhibit the rate of stem cell self-renewal in the SGZ of the DG of adult rats. Representative images identifying triple positive (BrdU+/Sox2+/S100B+) new adult-born astrocytes (arrows) and double-positive (BrdU+/Sox2+/S100B-) new adult-born stem cells (arrowheads). Statistical analysis was performed using one-way ANOVA with Bonferroni's post hoc test, n = 12 rats per group. Scale bar = 20mum.From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.Psychoneuroendocrinology. 2015 Jan;51:431-9.)

Application Data (Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.Image caption:Ghrelin enhances spatial pattern separation and adult hippocampal neurogenesis in adult rats.(D) Representative images of DCX+ immature neurones and new adult-born DG neurones (white arrows) co-expressing NeuN+ and BrdU+ (yellow). Scale bar = 200mum.From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.Psychoneuroendocrinology. 2015 Jan;51:431-9.)

Application Data (Mouse Anti Bromodeoxyuridine antibody, clone Bu20a used to label DNA synthesis in cultured mouse astrocytes by immunofluorescence.Image caption:Astrocyte response to injury involves proliferation which is attenuated by TTX and KB-R7943. (a) GFAP positive cultured rat cortical astrocytes (green) exhibit BrdU immunolabelling (red), seen in resting cells (top row) and scratched cells (second row). There is an increase in BrdU-positive cells along the edge of a scratch, which is attenuated with TTX and KB-R7943 treatment (third and fourth rows, respectively). Scale bar 200 mum. (b) After 24 h, there was a significant 77 +/- 9% increase in proliferation amongst cells along the edge of a wound (n=7) compared to cells in unscratched cultures (n=8), as measured by BrdU staining. Compared to untreated cells, TTX decreased proliferation by 54 +/- 4%, while KB-R7943 decreased proliferation by 46 +/- 9%. *p)

Application Data (Brdu-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution for 1 hour at room temperature. Goat anti Mouse IgG (H/L) DyLight 649 conjugated was used as the secondary detection reagent at a 1/50 dilution. PUREBLU Hoechst 33342 (1351304) was used to stain total DNA. B. BrdU-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, at a 1/100 dilution overnight at 4 degree C. Goat anti Mouse IgG (H/L) FITC conjugated antibody was used at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.)

BrdU, Monoclonal Antibody (Cat# AAA12259)

Full Name

Mouse Anti BrdU

Applications

Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cellsusing Fatty Acid Synthase antibody(AAA10626). Blue: DAPI for nuclear staining)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using FASN antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using FASN antibody at dilution of 1:100 (40x lens).)

IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of H460 cells, using 3 ug Fatty Acid Synthase antibody (AAA10626). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase antibody (AAA10626) at a dilition of 1:1000.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and Fatty Acid Synthase knockout (KO) 293T cells, using Fatty Acid Synthase antibody (AAA10626) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of extracts of H460 cells, using FASN antibody at 1:200 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Fatty Acid Synthase, Polyclonal Antibody (Cat# AAA10626)

Full Name

FASN Polyclonal Antibody

Gene Names

FASN; FAS; OA-519; SDR27X1

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

Purity

Affinity purification

Pricing

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Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:Morphology and P450 arom immunoreactivity of tumoral region in human testis with seminoma. A-B: Haematoxylin-eosin staining. C-D: Strong P450 arom immunoreactivity in cytoplasm of neoplastic cells (Nc) and unstained lymphocytes (L). Insert: absorption control. Scale bars: A, 20 um; B-C, 12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in rat prostate by immunohistochemistry on paraffin embedded sectionsImage caption:Immunohistochemical staining of aromatase in prostate of control and BPA-treated rats at doses of 25, 50, 300, or 600 ug/Kg/d for 4 days.From: Castro B, S¡nchez P, Torres JM, Preda O, del Moral RG, Ortega E. Bisphenol A exposure during adulthood alters expression of aromatase and 5a-reductase isozymes in rat prostate. PLoS One. 2013;8(2):e55905.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detaction of aromatase in human tissues by Immunohistochemistry on paraffin sectionsImage caption:P450 arom immunoreactivity in testicular region adjacent to seminoma and in controls A: Intense aromatase immunostaining in IGCN cells. B: Placental-like alkaline phosphatase staining of IGCN basal cells C: Strong aromatase immunoreactivity of interstitial Leydig cells in normal testis (Lc) D: Intense immunostaining of luteal cells (Luc) in ovarian tissue Scale bars: A-B, 8 um; C,12.5 um; D, 5 um.From: Rago V, Romeo F, Aquila S, Montanaro D, And S, Carpino A. Cytochrome P450 aromatase expression in human seminoma. Reprod Biol Endocrinol. 2005 Dec 22;3:72.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 use for the detection of aromatase in KGN cells by western blottingImage caption:Effect of H89 on aromatase and BRCA1 expression in KGN cells. A, The human ovarian granulosa cell line KGN were treated with control (DMSO), 20 mM H89, 50 mM PD98059, 200 nM Wortmannin, 100 nM Rapamycin 100 nM, and cells were harvested 24 hours after treatment. Immunoblotting was performed using specific antibodies against aromatase, BRCA1 and a-tubulin; B, Aromatase and BRCA1 mRNA levels were measured by real-time RT-PCR. Gapdh was used for normalizing the real time PCR results. The data are expressed as a fold (Aromatase mRNA), or a percentage (BRCA1 mRNA) relative to the DMSO-treated control.From: Ghosh S, Lu Y, Hu Y. A Role of CREB in BRCA1 Constitutive Promoter Activity and Aromatase Basal Expression. Int J Biomed Sci. 2008 Dec 15;4(4):260-265.)

Application Data (Western blot analysis of human placenta extract probed with Mouse anti Human Cytochrome P450 Aromatase followed by F(ab')2 Rabbit anti Mouse IgG:HRP)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in human lung tumors by immunohistochemistry on formalin fixed paraffin embedded tissue.Image caption:Representative immunohistochemistry of lung tumors for ERa, ERbeta, aromatase, EGFR and PR.From: Stabile LP, Dacic S, Land SR, Lenzner DE, Dhir R, Acquafondata M, Landreneau RJ, Grandis JR, Siegfried JM. Combined analysis of estrogen receptor beta-1 and progesterone receptor expression identifies lung cancer patients with poor outcome. Clin Cancer Res. 2011 Jan 1;17(1):154-64.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in porcine spermatozoa by immunofluorescenceImage caption:Immunofluorescence labelling of androgen and estrogen receptors in pig spermatozoa: A) AR red fluorescence in sperm proximal mid-piece. B) P450arom red brilliant light in the proximal tail of sperm with a diffuse labelling in the distal tail. C) ERa red fluorescence in the sperm mid-piece, together with a faint labelling in the tail. D) ERbeta green intense light in the sperm acrosomal region. Inserts: immunonegative absorption controls. Scale bars: 5 um.From: Rago V, Aquila S, Panza R, Carpino A. Cytochrome P450arom, androgen and estrogen receptors in pig sperm. Reprod Biol Endocrinol. 2007 Jun 6;5:23.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in sheep tissues by immunohistochemistry on paraffin sectionsImage caption:a and b) Low- (a) and higher- (b) power sections of the fetal end of a placentome in G1 stained with the prolactin antiserum and showing the intensely stained binucleate cells scattered along the trophoblast, but with a tendency to be more densely accumulated towards the fetal end (scale bars a=200?um; b=150?um). (c) High-power section of the trophoblast -endometrium interface in a placentome in G2 stained with the prolactin antiserum. Note the patchy staining of the thin, elongated endometrial epithelial cells (arrowed) in close contact with the intensely stained binucleate trophoblast cells (scale bar=40?um). (d) Low-power section of the intercotyledonary allantochorion stained with the prolactin antiserum and showing the relatively dense accumulation of binucleate trophoblast cells (arrowed) which remain unstained in this region of the placenta (scale bar=100?um). (e) High-power section at the edge of a placentome of G1 showing positive staining of the uninucleate trophoblast by the 3beta-HSD antiserum. The binucleate cells (arrowed) remain unstained (scale bar=40?um). (f) Section of the distended endometrial glands at the lateral border of a placentome in G2 stained with 3beta-HSD. The basal portions of the endometrial cells lining the glands stain strongly as does the secretory material adhered to the luminal surface of the cells and the accumulated coagulum, possibly as a result of ˜stickiness' of the material (scale bar=150?um). (g) High-power section of the intercotyledonary region of the allantochorion in G1 showing positive staining of the cytoplasm of the uninucleate, but not binucleate trophoblast by the 17,20 lyase antiserum (scale bar=40?um). (h) Section at the fetal end of a placentome from G2 remaining unstained by the aromatase antiserum (scale bar=150?um). (i) Section of near-term sheep placenta showing strong, positive staining of the uninucleate, but not binucleate, trophoblast by the aromatase antiserum (scale bar=150?um). (j) High-power section at the fetal end of a placentome in G1 showing strong positive staining of the uninucleate trophoblast by the PR antiserum. The binucleate cells (arrowed) remain unstained (scale bar=40?um).From: Wilsher S, Stansfield F, Greenwood RE, Trethowan PD, Anderson RA, Wooding FB, Allen WR. Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis). Reproduction. 2013 May 21;145(6):541-54.)

Application Data (Published customer image: Mouse anti Human cytochrome p450 aromatase antibody, clone H4 used for the detection of aromatase in human synovial tissue by immunohistochemistry on cryosectionsImage caption:Aromatase expression in synovial tissue and endogenous steroid hormone release from superfused synovium. (a) Immunohistochemistry of aromatase in one OA and one RA patient. Using the respective control antibody revealed no staining of positive cells (not shown). Magnification: 400x. (b) Density of aromatase-positive cells in OA (open bars, n = 20) and RA patients (hatched bars, n = 16). (c) Spontaneously released E2, E3, and free testosterone from standardized superfused pieces of synovial tissue of OA (open bars, n = 20) and RA patients (hatched bars, n = 18). (b,c) Values are given as box blots with the 5th, 25th, 50th (median), 75th, and 95th percentile when applicable. OA, osteoarthritis; RA, rheumatoid arthritis. Other abbreviations are as given in the legend to Fig. 1.From: Schmidt M, Weidler C, Naumann H, Anders S, Sch¶lmerich J, Straub RH. Androgen conversion in osteoarthritis and rheumatoid arthritis synoviocytes--androstenedione and testosterone inhibit estrogen formation and favor production of more potent 5alpha-reduced androgens. Arthritis Res Ther. 2005;7(5):R938-48.)

CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)

Full Name

MOUSE ANTI HUMAN CYTOCHROME P450 AROMATASE

Gene Names

CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

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5 results for " OA" - showing 1-5

FASN, Antibody (Cat# AAA17951)

Fatty Acid Synthase, Monoclonal Antibody (Cat# AAA30258)

BrdU, Monoclonal Antibody (Cat# AAA12259)

Fatty Acid Synthase, Polyclonal Antibody (Cat# AAA10626)

CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)

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5 results for " OA" - showing 1-5

FASN, Antibody (Cat# AAA17951)

Fatty Acid Synthase, Monoclonal Antibody (Cat# AAA30258)

BrdU, Monoclonal Antibody (Cat# AAA12259)

Fatty Acid Synthase, Polyclonal Antibody (Cat# AAA10626)

CYTOCHROME P450 AROMATASE, Monoclonal Antibody (Cat# AAA12113)

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