Search results for " Neural Stem Cell Marker"

4 results for " Neural Stem Cell Marker" - showing 1-4

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IF (Immunofluorescence) (Immunofluorescence analysis of rat brain using GFAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain using GFAP Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using GFAP Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human brain using GFAP Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using GFAP Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of U-251MG cells, using GFAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GFAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

GFAP, Monoclonal Antibody (Cat# AAA28378)

Full Name

GFAP Rabbit mAb

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody . Western blot was performed from the immunoprecipitate using Vimentin antibody at a dilition of 1:1000.)

IF (Immunofluorescence) (Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). Vimentin (: Vimentin Rabbit mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using Vimentin antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat spleen using Vimentin antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Vimentin, Monoclonal Antibody (Cat# AAA28380)

Full Name

[KO Validated] Vimentin Rabbit mAb

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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Application Data (Figure 6: Immunofluorescence staining of a 1 month old zebrafish embryo)

Application Data (Figure 5: Immunofluorescence staining of the developing neural tube in a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining)

Application Data (Figure 4: Immunohistochemistry on paraffin section of human colon)

Application Data (Figure 3: MUB1900L2: Frozen section of swine colon immunostained with RV202-HRP (1:200))

Application Data (Figure 2: MUB1900L1: Frozen section of swine colon immunostained with RV202-FITC (1:200))

Application Data (Figure 1: MUB1900P: Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells)

Vimentin, Monoclonal Antibody (Cat# AAA14566)

Full Name

Mouse anti Vimentin, conjugated with HRP

Gene Names

VIM; CTRCT30

Reactivity

Canine, Chicken, Caprine, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish

Applications

FC, IHC, IHC, frozen, IHC, paraffin, WB

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: mouse brain tissue lysates,Lane 4: 293T whole Cell lysates,Lane 5: 293T whole Cell lysates,Lane 6: HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Musashi 1/Msi1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Musashi 1/Msi1 at approximately 39KD. The expected band size for Musashi 1/Msi1 is at 39KD.)

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)

Full Name

Anti-Musashi 1/Msi1 Picoband Antibody

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins

Applications

IHC, WB

Purity

Immunogen affinity purified

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4 results for " Neural Stem Cell Marker" - showing 1-4

GFAP, Monoclonal Antibody (Cat# AAA28378)

Vimentin, Monoclonal Antibody (Cat# AAA28380)

Vimentin, Monoclonal Antibody (Cat# AAA14566)

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)

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4 results for " Neural Stem Cell Marker" - showing 1-4

GFAP, Monoclonal Antibody (Cat# AAA28378)

Vimentin, Monoclonal Antibody (Cat# AAA28380)

Vimentin, Monoclonal Antibody (Cat# AAA14566)

Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)

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