Search results for " NK Cells"

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LAT, Polyclonal Antibody (Cat# AAA31413)

Full Name

Phospho-LAT (Tyr161) Antibody

Gene Names

LAT; LAT1; pp36

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Zebrafish (83%), Bovine (100%), Horse (91%), Sheep (100%), Rabbit (80%), Dog (82%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Sample: Recombinant PRF1, Human;Antibody: Rabbit Anti-Human PRF1 Ab)

Activity (Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. Besides, Calreticulin (CRT) has been identified as an interactor of PRF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PRF1 and recombinant human CRT. Briefly, PRF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CRT-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRF1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PRF1 and CRT was shown in Figure 1, and this effect was in a dose dependent manner.The activity of recombinant PRF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant human PRF1 with 0.9% NaCl, add 50uL a serial dilution of PRF1, 10uL 0.1M CaCl2 to each well, then add 50uL 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 10uL 0.9% NaCl to reaplace CaCl2 in control wells. The plate is incubated for 20 hours at 37 degree C, 5% CO2. The results are shown in Figure 2. It was obvious that the minimal effective concentration of PRF1 is 12.5ug/mL.)

Perforin 1 (PRF1), Active Protein (Cat# AAA21142)

Full Name

Active Perforin 1 (PRF1)

Gene Names

PRF1; P1; PFP; HPLH2

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>80%

Pricing

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)

CD314, Monoclonal Antibody (Cat# AAA11960)

Full Name

MOUSE ANTI HUMAN CD314

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)

CD314, Monoclonal Antibody (Cat# AAA11907)

Full Name

MOUSE ANTI HUMAN CD314:Low Endotoxin

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

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CD25, Monoclonal Antibody (Cat# AAA11965)

Full Name

MOUSE ANTI RAT CD25

Gene Names

Il2ra; IL2RAC

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using YAP1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

YAP1, Polyclonal Antibody (Cat# AAA28345)

Full Name

YAP1 Rabbit pAb

Gene Names

YAP1; YAP; YKI; YAP2; YAP65

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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CD25, Monoclonal Antibody (Cat# AAA11966)

Full Name

MOUSE ANTI RAT CD25

Gene Names

Il2ra; IL2RAC

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

JAK3, Polyclonal Antibody (Cat# AAA31404)

Full Name

Phospho-JAK3 (Tyr904) Antibody

Gene Names

JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (Published clone specific image Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p)

CD172a, Monoclonal Antibody (Cat# AAA11875)

Full Name

MOUSE ANTI RAT CD172a:FITC

Gene Names

Sirpa; Bit; Ptpns1; SHPS-1

Applications

Flow Cytometry

Pricing

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CD25, Monoclonal Antibody (Cat# AAA12044)

Full Name

MOUSE ANTI RAT CD25:RPE

Gene Names

Il2ra; IL2RAC

Applications

Flow Cytometry

Pricing

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CD335, Monoclonal Antibody (Cat# AAA12253)

Full Name

MOUSE ANTI PIG CD335

Reactivity

Pig

Applications

Flow Cytometry, Immunofluorescence

Pricing

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)

CD314, Monoclonal Antibody (Cat# AAA12043)

Full Name

MOUSE ANTI HUMAN CD314:RPE

Applications

Flow Cytometry

Pricing

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JAK3, Polyclonal Antibody (Cat# AAA31405)

Full Name

Phospho-JAK3 (Tyr981) Antibody

Gene Names

JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (100%), Sheep (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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CD335, Monoclonal Antibody (Cat# AAA12251)

Full Name

MOUSE ANTI PIG CD335: APC

Reactivity

Pig

Applications

Flow Cytometry

Pricing

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)

CD314, Monoclonal Antibody (Cat# AAA11874)

Full Name

MOUSE ANTI HUMAN CD314:FITC

Applications

Flow Cytometry

Pricing

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Application Data (Published clone specific image Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p)

CD172a, Monoclonal Antibody (Cat# AAA11967)

Full Name

MOUSE ANTI RAT CD172a

Gene Names

Sirpa; Bit; Ptpns1; SHPS-1

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry, Western Blot

Pricing

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Application Data (Published clone specific image Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p)

CD172a, Monoclonal Antibody (Cat# AAA11968)

Full Name

MOUSE ANTI RAT CD172a

Gene Names

Sirpa; Bit; Ptpns1; SHPS-1

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry, Western Blot

Pricing

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD16:Alexa Fluor 647 (AAA11921))

CD16, Monoclonal Antibody (Cat# AAA11921)

Full Name

MOUSE ANTI HUMAN CD16

Gene Names

FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb

Applications

Flow Cytometry, Immunoprecipitation

Pricing

OX40L, Recombinant Antibody (Cat# AAA14184)

Full Name

Anti-OX40L [R4930 (Oxelumab)]

Gene Names

TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B

Reactivity

Human

Applications

Flow Cytometry

Purity

Protein A Affinity Purified

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA12049)

Full Name

MOUSE ANTI RAT CD8 ALPHA:RPE

Applications

Flow Cytometry

Pricing

TIGIT, Polyclonal Antibody (Cat# AAA28803)

Full Name

TIGIT Antibody

Gene Names

TIGIT; VSIG9; VSTM3; WUCAM

Reactivity

Human, Mouse

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11982)

Full Name

MOUSE ANTI RAT CD8 ALPHA

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11983)

Full Name

MOUSE ANTI RAT CD8 ALPHA

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Pricing

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Application Data (Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)

CD178, Monoclonal Antibody (Cat# AAA11962)

Full Name

MOUSE ANTI HUMAN CD178

Gene Names

FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Pricing

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FCM (Flow Cytometry) (CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

CB2, Polyclonal Antibody (Cat# AAA28672)

Full Name

CB2 Antibody (C-term)

Gene Names

CNR2; CB2; CX5; CB-2

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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HLA-A, Monoclonal Antibody (Cat# AAA13821)

Full Name

HLA-A (MHC I) Mouse Monoclonal Antibody

Gene Names

HLA-B; AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HEL-S-83; HLA-DRB1; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11981)

Full Name

MOUSE ANTI RAT CD8 ALPHA

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Pricing

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IF (Immunofluorescence) (Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite’ Mounting Medium with DAPI (nuclei, blue).)