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17 results for " Lyn Antibody" - showing 1-17
WB (Western Blot)
(Figure 1. Western blot analysis of Lyn using anti-Lyn antibody (AAA19150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lyn antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lyn at approximately 59KD. The expected band size for Lyn is at 59KD.)
Lyn, Polyclonal Antibody (Cat# AAA19150)
Full Name
Anti-Lyn Picoband Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(AAA31092 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
STAT3, Polyclonal Antibody (Cat# AAA31092)
Full Name
STAT3 Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(WB Suggested Anti-LYN Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysateLYN is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)
LYN, Polyclonal Antibody (Cat# AAA23560)
Full Name
LYN antibody - N-terminal region
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
Western Blot
Purity
Affinity Purified
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CD22, Polyclonal Antibody (Cat# AAA31418)
Full Name
Phospho-CD22 (Tyr842) Antibody
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (83%)
Predicted Reactivity: Horse (83%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Jak2, Polyclonal Antibody (Cat# AAA31311)
Full Name
Phospho-Jak2 (Tyr1008) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p27 Kip1, Polyclonal Antibody (Cat# AAA31388)
Full Name
Phospho-p27 Kip1 (Ser178) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
HCLS1, Monoclonal Antibody (Cat# AAA25416)
Full Name
HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (HRP)
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p27 Kip1, Polyclonal Antibody (Cat# AAA31387)
Full Name
Phospho-p27 Kip1 (Thr157) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(WB Suggested Anti-HCLS1 Antibody Titration: 1.25ug/mlPositive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat)
HCLS1, Polyclonal Antibody (Cat# AAA23399)
Full Name
HCLS1 antibody - N-terminal region
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Protein A purified
Pricing
WB (Western Blot)
(WB Suggested Anti-HCLS1 Antibody Titration: 1.25ug/mlPositive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat)
HCLS1, Polyclonal Antibody (Cat# AAA23398)
Full Name
HCLS1 antibody - N-terminal region
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
Application Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
HCLS1, Monoclonal Antibody (Cat# AAA25122)
Full Name
HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (FITC)
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
HCLS1, Monoclonal Antibody (Cat# AAA25711)
Full Name
HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (PE)
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
HCLS1, Monoclonal Antibody (Cat# AAA24825)
Full Name
HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (Biotin)
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
HCLS1, Monoclonal Antibody (Cat# AAA24530)
Full Name
HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) APC
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
HCLS1, Monoclonal Antibody (Cat# AAA24234)
Full Name
HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (AP)
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(AAA30990 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Lyn, Polyclonal Antibody (Cat# AAA30990)
Full Name
Phospho-Lyn (Tyr508) Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of STAT3 phosphorylation expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
STAT3, Polyclonal Antibody (Cat# AAA31042)
Full Name
Phospho-STAT3 (Ser727) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing