Search results for " Leishmania"

5 results for " Leishmania" - showing 1-5

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Application Data (Published customer image: Effects of EDTA versus media storage of patient blood samples on flow cytometric results. Peripheral blood cells were (a) stained immediate following collection with CD11b and CADO48A or maintained in EDTA collection tubes for (b) 24 or (c) 48 hours or in media for (d) 24 or (e) 48 hours prior to staining with CD11b and CADO48A. Both EDTA and media samples were kept refrigerated. These findings show a decrease over time of population distinction in EDTA with minimal changes when cells are kept in media up to 48 hours. All cells were gated on P1.From: Sherger et al. BMC Veterinary Research 2012 8:209)

Application Data (Published customer image: Staining characteristics and evaluation of two commercially available canine granulocytic antibodies of canine peripheral blood. (a) Cells were analyzed according to forward and side scatter. Cells were either gated to include all live and dead (no gate), all live cells including lymphocytes (R1, lymphocytes are circled) and all myeloid cells (P1). (b) Cells were labeled using canine anti-CD11b and either (a) CADO48A or (b) DH59B with the same concentration of secondary antibody FITC. As can be seen in (a), CADO48A allows visualization of an additional cell population as compared to DH59B (b). These results were repeatable with multiple blood samples from different dogs. The cells in (b) were gated on P1 as indicated in (a).From: Sherger et al. BMC Veterinary Research 2012 8:209)

Application Data (Published customer image: Mouse anti-CD11b and Gr-1 antibodies cross-react with canine samples. Fresh PBMCs from healthy dog and cancer patients were isolated by Ficoll, stained with anti-mouse CD11b and anti-mouse Gr-1 antibodies.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Published customer image: Increased percentage of CD11blow/CADO48Alow in tumor-bearing canine patients. The expression levels of CD11blow and CADO48Alow was evaluated from the peripheral blood of representative canine patients and show an increase of CD11blow/CADO48low in tumor-bearing canine patients.From: Sherger et al. BMC Veterinary Research 2012 8:209)

Application Data (Published customer image: Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14-MHCII- cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14-MHCII- cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14-MHCII- cells is shown at 63x magnification.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Published customer image: CD11b+CD14+MHCII- cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII- cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Published customer image: Gated myeloid subpopulations evaluated for patient population. All peripheral blood myeloid cells were evaluated using the P1 gate (non-lymphocytes) and subsequently gated based on relatively expression levels of CD11b and CADO48A where CADO48Ahi/CD11bhi (gate P2), CADO48Alow/CD11bhi (gate P3) and CADO48Alow/CD11blow (gate P4) likely represent granulocytes/neutrophils, monocytes and MDSCs, respectively.From: Sherger et al. BMC Veterinary Research 2012 8:209)

Application Data (Published customer image: Flow cytometer analysis of CD11b integrin expression and the binding of Leishmania promastigotes to canine monocytes. (A) Gate R1 was built separating monocytes from animals naturally infected (ANI) with L. chagasi by cells size (x axis) and granularity (y axis). (B) Gate with mononuclear cells (control) (C) CD11b positive cells; (D) CD11b expression during the L. chagasi binding to mononuclear cells with the presence of C5D serum. (E) CD11b expression during the L. chagasi binding to mononuclear cells with the absence of C5D serum.From: Sampaio et al. BMC Veterinary Research 2007 3:11)

Application Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Published customer image: Optimization of secondary antibody staining concentration for detection of CADO48A. Cells were stained with the primary antibody CADO48A alone followed by (a) 1:10 (b) 1:50 and (c) 1:100 dilutions of a secondary FITC antibody. Optimal detection was seen between 1:50 and 1:100 dilution with CADO48A alone. Secondary FITC concentrations of 1:50 (d) and 1:100 (e) to detect CADO48A on pre-labeled CD11b cells were then evaluated and demonstrated that a 1:50 concentration of FITC was optimal for optimal detection of distinct CADO48A+ cells. Based on these findings, a 1:50 FITC concentration was used in all clinical samples. All cells in these diagrams were gated on P1.From: Sherger et al. BMC Veterinary Research 2012 8:209)

CD11b, Monoclonal Antibody (Cat# AAA12092)

Full Name

MOUSE ANTI DOG CD11b

Gene Names

ITGAM; CD11B

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

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Application Data (Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)

Application Data (Published customer image: Mouse anti Human CD18 antibody, clone YFC118.3 used for immunofluorescence studies using formalin fixed paraffin embedded material following antigen retrieval with citrate buffer at pH6.0Image caption:Subretinal MPs accumulate on the retinal pigment epithelium in AMDA -D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E -G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.Data information: Controls omitting the primary antibody showed no staining apart from the autofluorescence. AZ: atrophic zone; LD: large drusen. Scale bar: 50 um (A -D); 10 um (E -H).From: Levy O, Calippe B, Lavalette S, Hu SJ, Raoul W, Dominguez E, Housset M, Paques M, Sahel JA, Bemelmans AP, Combadiere C, Guillonneau X, Sennlaub F. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. EMBO Mol Med. 2015 Jan 20. pii: e201404524.)

Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD18:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD18)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD18:Biotin)

Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD18:RPE)

Application Data (Published customer image: Rat anti Human CD18 antibody,clone YFC118.3 used for flow cytometryImage caption: LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing œhigh affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.From: Dilek N, Poirier N, Hulin P, Coulon F, Mary C, et al. (2013) Targeting CD28, CTLA-4 and PD-L1 Costimulation Differentially Controls Immune Synapses and Function of Human Regulatory and Conventional T-Cells. PLoS ONE 8(12): e83139.)

Application Data (Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)

CD18, Monoclonal Antibody (Cat# AAA12051)

Full Name

RAT ANTI HUMAN CD18:RPE

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Applications

Flow Cytometry

Pricing

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Application Data (Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647 (AAA11986A647))

Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD18:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD18)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD18:Biotin)

Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD18:RPE)

CD18, Monoclonal Antibody (Cat# AAA11986)

Full Name

RAT ANTI HUMAN CD18

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Reactivity

Dog, Guinea Pig

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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Application Data (Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)

Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD18:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD18)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD18:Biotin)

Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD18:RPE)

CD18, Monoclonal Antibody (Cat# AAA11987)

Full Name

RAT ANTI HUMAN CD18

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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Application Data (Staining of human peripheral blood granulocytes with CD18: Alexa Fluor 647)

Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD18:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD18)

Application Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD18:Biotin)

Application Data (Staining of human peripheral blood granulocytes with Rat anti Human CD18:RPE)

CD18, Monoclonal Antibody (Cat# AAA11881)

Full Name

RAT ANTI HUMAN CD18:FITC

Gene Names

ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1

Applications

Flow Cytometry

Pricing

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5 results for " Leishmania" - showing 1-5

CD11b, Monoclonal Antibody (Cat# AAA12092)

CD18, Monoclonal Antibody (Cat# AAA12051)

CD18, Monoclonal Antibody (Cat# AAA11986)

CD18, Monoclonal Antibody (Cat# AAA11987)

CD18, Monoclonal Antibody (Cat# AAA11881)

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5 results for " Leishmania" - showing 1-5

CD11b, Monoclonal Antibody (Cat# AAA12092)

CD18, Monoclonal Antibody (Cat# AAA12051)

CD18, Monoclonal Antibody (Cat# AAA11986)

CD18, Monoclonal Antibody (Cat# AAA11987)

CD18, Monoclonal Antibody (Cat# AAA11881)

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