Search results for " Immunol"

4 results for " Immunol" - showing 1-4

Display

< >

<< Previous Next >>

Application Data (AlexaFluor 700 conjugated Mouse anti Human CD45RA antibody used for the phenotypic characterization of CD8 expressing T cells by flow cytometry.Image caption:CD8 T cell subpopulation distribution in the blood and endocervix.(A) A diagram of Sallusto and Lanzavecchia’s classification scheme of CD8 T cells based on CD45RA and CCR7 expression and relative perforin expression of the different subpopulation, (B) distribution of the different CD8 T cell subpopulations in a representative C. trachomatis-negative and C. trachomatis-positive women (C-D) Distribution of different CD8 T cells in the blood (C), and in the endocervix (D) of 16 women (n=16). Using flow cytometric analyses, CD8 T cells were identified in a population of cells from PBMC and endocervical cells by gating for CD3+CD8+ cells. CD8 T cells were further characterized based on CD45RA and CCR7 expression. Naïve cells are CD45RA+ CCR7+, central memory (TCM) cells are CD45RA-CCR7+, effector memory (TEM) cells are CD45RA- CCR7-, and terminally differentiated effector memory (TEMRA) cells are CD45RA+ CCR7-. The majority of CD8 T cells in the endocervix are TEM CD8 subtype.From: Ibana JA, Myers L, Porretta C, Lewis M, Taylor SN, Martin DH, Quayle AJ.The major CD8 T cell effector memory subset in the normal and Chlamydia trachomatis-infected human endocervix is low in perforin.BMC Immunol. 2012 Dec 7;13:66.)

CD45RA, Monoclonal Antibody (Cat# AAA12272)

Full Name

Mouse Anti Human CD45RA: Amethyst Orange

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromotography on protein A from tissue culture supernatant.

Pricing

< >

<< Previous Next >>

Application Data (Mouse anti Human HLA DP/DQ/DR antibody, clone WR18 used for the evaluation of HLA class II histocompatibility antigen expression on cell populations by flow cytometry.Image caption:DC differentiation from H1 hESC.Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.From: Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ.Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012;2012:172420.doi: 10.1155/2012/172420.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the treatment on monocytes in vitrodetection of MHC class II onImage caption:Ligand engagement of HLA class II molecules up regulates MyD88, IL-1R1, and TNF-? in CD14+ human monocytes treated with SEB, SEA, or mAb directed against MHC-class II molecules. (A) Transcriptional activation of MyD88, IL-1R1AcP and TNF-? in HLA-DR positive primary monocytes treated with 200 ng SEB/ml (optimum dose), mAbs (10 µg/ml, optimum dose) directed against MHC-class II molecule (anti-DR-DP-DQ or LB3.1) or unrelated control antibody OKT3 was examined by semi-quantitative RT-PCR. Data shown is one of 3 similar experiments; (B) Agonists binding to TLR4 or HLA class II molecules on CD14+ monocytes induced transcriptional up regulation of MyD88. Real time RT-PCR was used to determine relative expression of MyD88 normalized to the expression of ?-actin. Expression levels are determined as means ± SD. Data presented as one of 3 similar experiments. Significance compared to untreated control (*) was assigned as P?0.0001. (C) MHC class II molecule dependence of SEB- induced TNF- ? gene expression. Pretreatment of monocytes in ice with anti-DR-DP-DQ at optimum dose (10 ug/ml) followed by SEB stimulation resulted in reduced TNF- ? gene expression. Transcriptional activation of TNF- ? expression normalized to the expression of ?-actin. Expression levels of TNF- ? are expressed as means ± SD. Significance was assigned (*) or (**) as P values ?0.003 comparing untreated vs treatment groups with anti-DR-DP-DQ, SEB or SEB vs anti-DR-DP-DQ+SEB respectively. (D) Confocal images show expression of HLA-DR (green) and intracellular MyD88 (red) proteins in CD14+ monocytes treated with HLA class II- ligands or control antibody OKT3 for 16 h; Scale bar = 5 ?m; (E) intracellular expression of MyD88 protein in activated monocytes. Primary monocytes (CD14+, CD3-) were activated as described earlier, permeabilized and labeled with primary MyD88 antibody followed by PE-labeled secondary antibody and analyzed by flow cytometry. Histogram represents a MHC class II ligand-induced increase in expression of MyD88 protein compared to non-MHC class II ligand (OKT3).From: Kissner TL, Ruthel G, Alam S, Ulrich RG, Fernandez S, et al. (2011) Activation of MyD88 Signaling upon Staphylococcal Enterotoxin Binding to MHC Class II Molecules.PLoS ONE 6(1): e15985.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the evaluation of MHC class II expression on dendritic cells by flow cytometry.Image caption:Time course of DC differentiation from H1 hESC. Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (a) Cells harvested at day 20 of culture showing expressing of CD45 but lack of myeloid commitment, as evidenced by staining for CD13, CD14, and CD11c. Open histograms show levels of background staining using isotype-matched control antibodies. (b) Appearance of CD45int cells at day 27 of culture, accompanied by the upregulation of myeloid-specific markers. (c) Photomicrograph, taken at day 28 of culture, showing the morphology of DC, including veils of cytoplasm and long dendrites (inset) (×40 magnification). (d) Cells harvested at day 33 of culture, showing the appearance of a CD45hi population containing predominantly DC progenitors expressing CD14, CD11c, CD86 and MHC class I. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.From: Kathryn M. Silk, Alison J. Leishman, Kevin P. Nishimoto, Anita Reddy, and Paul J. Fairchild, “Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype,”Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 172420, 11 pages, 2012.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

Full Name

Mouse Anti Human HLA DP DQ DR: Amethyst Orange

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Pricing

< >

<< Previous Next >>

Application Data (Rat anti MouseCD45R antibody, clone RA3-6B2 used for the demonstration of B cells in murine spinal column by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Representative images of key immunohistochemical markers run on a longitudinally sectioned, decalcified spinal column from an EAE mouse with a clinical EAE score of 4.(A) HE, (B) B220 immunohistochemistry for (B) cells, (C) F4/80 immunohistochemistry for macrophages (arrow indicates area highlighted in inset, inset bar = 20 ?m), (D) CD3 immunohistochemistry for T cells. Bars = 200 ?m.From: Gibson-Corley KN, Boyden AW, Leidinger MR, Lambertz AM, Ofori-Amanfo G, Naumann PW, Goeken JA, Karandikar NJ.A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.PeerJ. 2016 Jan 26;4:e1600.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)