Search results for " GABA A Receptor Associated Protein"

2 results for " GABA A Receptor Associated Protein" - showing 1-2

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human mammary cancer using GABARAP antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GABARAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

GABARAP, Polyclonal Antibody (Cat# AAA28252)

Full Name

GABARAP Polyclonal Antibody

Gene Names

GABARAP; MM46; ATG8A; GABARAP-a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U251 cells using anti-GABARAP antibody (AAA19250).Overlay histogram showing U251 cells stained with AAA19250 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GABARAP Antibody (AAA19250, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HEPA1-6 cells using anti-GABARAP antibody (AAA19250).Overlay histogram showing HEPA1-6 cells stained with AAA19250 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GABARAP Antibody (AAA19250, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of human renal carcinomar tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of GABARAP using anti-GABARAP antibody (AAA19250).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human HELA whole cell lysatesLane 5: human U87 whole cell lysatesLane 6: human HEPG2 whole cell lysatesLane 7: human U937 whole cell lysatesLane 8: rat liver tissue lysatesLane 9: rat pancreas tissue lysatesLane 10: mouse liver tissue lysates, Lane 11: mouse pancreas tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GABARAP antigen affinity purified polyclonal antibody (Catalog # AAA19250) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for GABARAP at approximately 18KD. The expected band size for GABARAP is at 18KD.)

GABARAP, Polyclonal Antibody (Cat# AAA19250)

Full Name

Anti-GABARAP Antibody

Gene Names

GABARAP; MM46; ATG8A; GABARAP-a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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2 results for " GABA A Receptor Associated Protein" - showing 1-2

GABARAP, Polyclonal Antibody (Cat# AAA28252)

GABARAP, Polyclonal Antibody (Cat# AAA19250)

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2 results for " GABA A Receptor Associated Protein" - showing 1-2

GABARAP, Polyclonal Antibody (Cat# AAA28252)

GABARAP, Polyclonal Antibody (Cat# AAA19250)

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