Search results for " DRP1 Antibody"

3 results for " DRP1 Antibody" - showing 1-3

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FCM (Flow Cytometry) (Figure 13. Flow Cytometry analysis of SiHa cells using anti-DRP1/DNM1L antibody (AAA19220).Overlay histogram showing SiHa cells stained with AAA19220 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRP1/DNM1L Antibody (AAA19220, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of A549 cells using anti-DRP1/DNM1L antibody (AAA19220).Overlay histogram showing A549 cells stained with AAA19220 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRP1/DNM1L Antibody (AAA19220, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 11. IF analysis of DRP1/DNM1L using anti- DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).DRP1/DNM1L was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DRP1/DNM1L Antibody (AAA19220) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (AAA19220).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: rat heart tissue lysatesLane 5: rat kidney tissue lysatesLane 6: rat liver tissue lysatesLane 7: rat brain tissue lysatesLane 8: mouse HEPA1-6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DRP1/DNM1L antigen affinity purified polyclonal antibody (Catalog # AAA19220) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for DRP1/DNM1L at approximately 82KD. The expected band size for DRP1/DNM1L is at 82KD.)

DRP1/DNM1L, Polyclonal Antibody (Cat# AAA19220)

Full Name

Anti-DRP1/DNM1L Antibody

Gene Names

DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with CRMP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining CRMP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CRMP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CRMP1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of CRMP1 on mouse brain tissue lysate using anti-CRMP1 antibody at 1/500 dilution.)

CRMP1, Monoclonal Antibody (Cat# AAA30425)

Full Name

CRMP1 Antibody

Gene Names

CRMP1; DRP1; DRP-1; CRMP-1; DPYSL1; ULIP-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence of HeLa celLauren Shoreusing AAA24076 (10ug/ml).)

Application Data (Detection limit for recombinant GST tagged UTRN is 0.1ng/ml using AAA24076 as a capture antibody.)

Utrophin, Monoclonal Antibody (Cat# AAA24076)

Full Name

Utrophin (UTRN, Dystrophin-related Protein 1, DRP1, DRP-1, DMDL, DRP)

Gene Names

UTRN; DRP; DMDL; DRP1

Reactivity

Human

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

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3 results for " DRP1 Antibody" - showing 1-3

DRP1/DNM1L, Polyclonal Antibody (Cat# AAA19220)

CRMP1, Monoclonal Antibody (Cat# AAA30425)

Utrophin, Monoclonal Antibody (Cat# AAA24076)

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3 results for " DRP1 Antibody" - showing 1-3

DRP1/DNM1L, Polyclonal Antibody (Cat# AAA19220)

CRMP1, Monoclonal Antibody (Cat# AAA30425)

Utrophin, Monoclonal Antibody (Cat# AAA24076)

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