Search results for " DNA Polymerase"

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IHC (Immunohistchemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

BRCA1, Polyclonal Antibody (Cat# AAA31415)

Full Name

Phospho-BRCA1 (Ser1497) Antibody

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (80%), Dog (80%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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PCNA, Polyclonal Antibody (Cat# AAA10631)

Full Name

PCNA Polyclonal Antibody

Gene Names

PCNA; ATLD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cells.)

POLK/DNA Polymerase Kappa, Polyclonal Antibody (Cat# AAA21279)

Full Name

Anti-POLK/DNA Polymerase Kappa Antibody

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

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POLR2A, Polyclonal Antibody (Cat# AAA31440)

Full Name

Phospho-POLR2A (Ser1616/Ser2) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Phospho-POLR2A (S2) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

POLR2A, Monoclonal Antibody (Cat# AAA29786)

Full Name

POLR2A (Phospho-S2) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)

POLK, Monoclonal Antibody (Cat# AAA24620)

Full Name

POLK (DNA Polymerase kappa, DINB1, DINB Protein) APC

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (PCNA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

PCNA, Polyclonal Antibody (Cat# AAA28705)

Full Name

PCNA Antibody (C-term)

Gene Names

PCNA; ATLD2

Reactivity

Human (Predicted Reactivity: Bovine, Hamster, Monkey, Rat)

Applications

Immunohistochemistry, Immunofluorescence, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

MCM3, Monoclonal Antibody (Cat# AAA26379)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (FITC)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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H2B, Polyclonal Antibody (Cat# AAA31349)

Full Name

Acetyl-H2B (Lys16) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

MCM3, Monoclonal Antibody (Cat# AAA26246)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (Biotin)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

MCM3, Monoclonal Antibody (Cat# AAA26644)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (PE)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PCNA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)

PCNA, Monoclonal Antibody (Cat# AAA29892)

Full Name

PCNA Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

MCM3, Monoclonal Antibody (Cat# AAA26512)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (HRP)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.)

DNA Polymerase beta, Monoclonal Antibody (Cat# AAA30365)

Full Name

DNA Polymerase beta Antibody

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry

Purity

ProA affinity purified

Pricing

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FOXO3A, Polyclonal Antibody (Cat# AAA31313)

Full Name

Acetyl-FOXO3A (Lys271) Antibody

Gene Names

FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)

POLK, Monoclonal Antibody (Cat# AAA24915)

Full Name

POLK (DNA Polymerase kappa, DINB1, DINB Protein) (Biotin)

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

H2B, Polyclonal Antibody (Cat# AAA31348)

Full Name

Acetyl-H2B (Lys5) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Histone H2B, Polyclonal Antibody (Cat# AAA31351)

Full Name

Phospho-Histone H2B (Tyr43) Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Histone H2A, Polyclonal Antibody (Cat# AAA31347)

Full Name

Acetyl-Histone H2A (Lys5) Antibody

Gene Names

HIST1H2AB; H2A/m; H2AFM

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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POLR2A, Monoclonal Antibody (Cat# AAA29787)

Full Name

POLR2A (Phospho-S5) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

MCM3, Monoclonal Antibody (Cat# AAA25980)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (AP)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Western Blot

Purity

Purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

PCNA, Monoclonal Antibody (Cat# AAA30026)

Full Name

PCNA Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)

POLK, Monoclonal Antibody (Cat# AAA25212)

Full Name

POLK (DNA Polymerase kappa, DINB1, DINB Protein) (FITC)

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)

POLK, Monoclonal Antibody (Cat# AAA24324)

Full Name

POLK (DNA Polymerase kappa, DINB1, DINB Protein) (AP)

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ICC (Immunocytochemistry) (Anti-MCM3 antibody, AAA11518, ICCICC: HELA Cell)

MCM3, Polyclonal Antibody (Cat# AAA11518)

Full Name

Anti-MCM3 antibody

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using POLR2A antibody at dilution of 1:100 (40x lens).)

POLR2A, Polyclonal Antibody (Cat# AAA28236)

Full Name

POLR2A Polyclonal Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purification

Pricing

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ChIP (Chromatin Immunoprecipitation) (Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-MCM3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the IRF-1 promoter.)

DNA replication licensing factor MCM3, Polyclonal Antibody (Cat# AAA18870)

Full Name

Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Reactivity

Human

Applications

EIA, WB, IHC, ChIP

Purity

Antigen Affinity Purified

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)

POLK, Monoclonal Antibody (Cat# AAA25802)

Full Name

POLK (DNA Polymerase kappa, DINB1, DINB Protein) (PE)

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HL-60 cells using anti-PARP antibody (AAA19346).Overlay histogram showing HL-60 cells stained with AAA19346 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PARP Antibody (AAA19346, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PARP, Monoclonal Antibody (Cat# AAA19346)

Full Name

Anti-PARP Antibody (monoclonal, 10G9)

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)

POLK, Monoclonal Antibody (Cat# AAA25506)

Full Name

POLK (DNA Polymerase kappa, DINB1, DINB Protein) (HRP)

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Histone H2B, Polyclonal Antibody (Cat# AAA31352)

Full Name

Acetyl-Histone H2B (Lys20) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IHC (Immunohistchemistry) (Fig.6. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, PCNA Monoclonal Antibod y(1D7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

PCNA, Monoclonal Antibody (Cat# AAA31541)

Full Name

Anti-PCNA Mouse Monoclonal Antibody (1D7)

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Pricing

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IHC (Immunohistochemistry-Paraffin) (AAA31235 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

DNA Polymerase theta, Polyclonal Antibody (Cat# AAA31235)

Full Name

DNA Polymerase theta Antibody

Gene Names

POLQ; PRO0327

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of DNA Polymerase zeta staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)