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410 results for " Conjugated Proteins" - showing 1-50
Western Blot (WB)
(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TREM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : TREM2 19-161aa Transfected 293T cell lysate)
TREM2, Monoclonal Antibody (Cat# AAA332953)
Full Name
Human TREM2 antibody
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Flow Cytometry
Purity
By protein-A affinity chromatography
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of CD72 using anti- CD72 antibody (AAA19329).CD72 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CD72 Antibody (AAA19329) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CD72, Polyclonal Antibody (Cat# AAA19329)
Full Name
Anti-CD72 Antibody
Gene Names
Cd72; CD72c; Ly-19; Ly-32; Lyb-2; Ly-m19
Reactivity
Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
histone H4, Polyclonal Antibody (Cat# AAA31295)
Full Name
Acetyl-histone H4 (Lys16) Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H4, Polyclonal Antibody (Cat# AAA31346)
Full Name
Acetyl-Histone H4 (Lys8) Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat, Pig, Bovine
Predicted Reactivity: Chicken (100%), Xenopus (100%)
Predicted Reactivity: Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Histone H3, Polyclonal Antibody (Cat# AAA31345)
Full Name
Acetyl-Histone H3 (Lys14) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H4, Polyclonal Antibody (Cat# AAA31350)
Full Name
Acetyl-Histone H4 (Lys91) Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat, Pig, Bovine
Predicted Reactivity: Chicken (100%), Xenopus (100%)
Predicted Reactivity: Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of THP-1 cells using anti-HDAC5 antibody (AAA19236).Overlay histogram showing THP-1 cells stained with AAA19236 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC5 Antibody (AAA19236, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HDAC5, Polyclonal Antibody (Cat# AAA19236)
Full Name
Anti-HDAC5 Antibody
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).)
RYK, Polyclonal Antibody (Cat# AAA28767)
Full Name
RYK Antibody
Gene Names
RYK; JTK5; RYK1; JTK5A; D3S3195
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
BRCA1, Polyclonal Antibody (Cat# AAA31415)
Full Name
Phospho-BRCA1 (Ser1497) Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (80%), Dog (80%)
Predicted Reactivity: Bovine (80%), Dog (80%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26902)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31356)
Full Name
Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
YAP, Polyclonal Antibody (Cat# AAA31298)
Full Name
Phospho-YAP (Ser941) Antibody
Gene Names
YAP1; YAP; YKI; YAP2; YAP65
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26899)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IHC (Immunohistochemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Caveolin 2, Polyclonal Antibody (Cat# AAA31397)
Full Name
Phospho-Caveolin 2 (Ser36) Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
GAPDH, Polyclonal Antibody (Cat# AAA28693)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
E-cadherin, Polyclonal Antibody (Cat# AAA31444)
Full Name
Phospho-E-cadherin (Ser844) Antibody
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (91%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26896)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31354)
Full Name
Acetyl-p53 (Lys373) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with MAP1LC3A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
MAP1LC3A, Monoclonal Antibody (Cat# AAA30089)
Full Name
MAP1LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-CGKI/PRKG1 antibody (AAA19246).Overlay histogram showing U251 cells stained with AAA19246 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGKI/PRKG1 Antibody (AAA19246, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
cGKI/PRKG1, Polyclonal Antibody (Cat# AAA19246)
Full Name
Anti-cGKI/PRKG1 Antibody
Gene Names
PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Smad2, Polyclonal Antibody (Cat# AAA31390)
Full Name
Phospho-Smad2 (Ser255) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IRF7, Polyclonal Antibody (Cat# AAA31458)
Full Name
Phospho-IRF7 (Ser477) Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26895)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Anti-tdTomato Ab conjugated to DyLight 550 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane;)
tdTomato, Polyclonal Antibody (Cat# AAA13880)
Full Name
Anti-tdTomato, DyLight550
Reactivity
Reacts with Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26894)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
POLR2A, Polyclonal Antibody (Cat# AAA31440)
Full Name
Phospho-POLR2A (Ser1616/Ser2) Antibody
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
HSF1, Polyclonal Antibody (Cat# AAA31391)
Full Name
Phospho-HSF1 (Ser307) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(EREG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EREG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
EREG, Polyclonal Antibody (Cat# AAA28771)
Full Name
EREG Antibody (C-term)
Gene Names
EREG; ER
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Smad2, Polyclonal Antibody (Cat# AAA31433)
Full Name
Phospho-Smad2 (Ser465+Ser467) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of MEK2/MAP2K2 using anti-MEK2/MAP2K2 antibody (AAA19230).MEK2/MAP2K2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-MEK2/MAP2K2 Antibody (AAA19230) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
MEK2/MAP2K2, Polyclonal Antibody (Cat# AAA19230)
Full Name
Anti-MEK2/MAP2K2 Antibody
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HEPG2 cells using anti-Alkaline phosphatase/ALPL antibody (AAA19231).Overlay histogram showing HEPG2 cells stained with AAA19231 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alkaline phosphatase/ALPL Antibody (AAA19231, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Alkaline phosphatase/ALPL, Polyclonal Antibody (Cat# AAA19231)
Full Name
Anti-Alkaline phosphatase/ALPL Antibody
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(PCNA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
PCNA, Polyclonal Antibody (Cat# AAA28705)
Full Name
PCNA Antibody (C-term)
Gene Names
PCNA; ATLD2
Reactivity
Human (Predicted Reactivity: Bovine, Hamster, Monkey, Rat)
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
NFAT2, Polyclonal Antibody (Cat# AAA31381)
Full Name
Phospho-NFAT2 (Ser294) Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A549 cells using anti-MCM4 antibody (AAA19257).Overlay histogram showing A549 cells stained with AAA19257 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM4 Antibody (AAA19257, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MCM4, Polyclonal Antibody (Cat# AAA19257)
Full Name
Anti-MCM4 Antibody
Gene Names
MCM4; NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(MYEF2 was immunoprecipitated using: Lane A:0.5 mg SH-SY5Y Whole Cell Lysate 4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 64 kDa Observed band size :64 kDa)
MYEF2, Polyclonal Antibody (Cat# AAA27756)
Full Name
Anti-MYEF2 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31377)
Full Name
p53 Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A549 cells using anti-Caveolin-2/CAV2 antibody (AAA19243).Overlay histogram showing A549 cells stained with AAA19243 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-2/CAV2 Antibody (AAA19243,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caveolin-2/CAV2, Polyclonal Antibody (Cat# AAA19243)
Full Name
Anti-Caveolin-2/CAV2 Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2A.X, Polyclonal Antibody (Cat# AAA31320)
Full Name
Acetyl-Histone H2A.X (Lys9) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
FCM (Flow Cytometry)
(SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SOCS1, Polyclonal Antibody (Cat# AAA28752)
Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of C6 cells using anti-non-muscle Myosin IIB/MYH10 antibody (AAA19262).Overlay histogram showing C6 cells stained with AAA19262 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-non-muscle Myosin IIB/MYH10 Antibody (AAA19262,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
non-muscle Myosin IIB/MYH10, Polyclonal Antibody (Cat# AAA19262)
Full Name
Anti-non-muscle Myosin IIB/MYH10 Antibody
Gene Names
MYH10; NMMHCB; NMMHC-IIB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
KAT3B/p300, Polyclonal Antibody (Cat# AAA31393)
Full Name
Phospho-KAT3B/p300 (Ser1834) Antibody
Gene Names
EP300; p300; KAT3B; RSTS2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U-87 cells using anti-ATP citrate lyase antibody (AAA11689).Overlay histogram showing U-87 cells stained with AAA11689 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP citrate lyase Antibody (AAA11689,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATP citrate lyase, Polyclonal Antibody (Cat# AAA11689)
Full Name
Anti-ATP citrate lyase Antibody
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Anti-SOST Antibody (N-term)at 1:2000 dilution + human kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SOST, Polyclonal Antibody (Cat# AAA28704)
Full Name
SOST Antibody (N-term)
Gene Names
SOST; CDD; VBCH; SOST1
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(IHC staining of immersion-fixed frozen section of mouse embryo (13 d.p.c.) using AAA14708 at 1.7ug/ml, with overnight incubation at 4°C. HRP-conjugated anti-goat secondary antibody and DAB staining were used for visualization of Reelin which in this sample was localized to the developing brain. The tissue section was counterstained with hematoxylin (blue).)
Reelin, Polyclonal Antibody (Cat# AAA14708)
Full Name
Reelin
Gene Names
RELN; RL; LIS2; PRO1598
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Pricing
Application Data
(Detection of Mouse anti Human CD300a in a flow cytometric analysis of human peripheral blood monocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (AAA12305))
IgG, Polyclonal Antibody (Cat# AAA12305)
Full Name
RABBIT F(ab')2 ANTI MOUSE IgG:FITC
Applications
Flow Cytometry
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rac1/cdc42, Polyclonal Antibody (Cat# AAA31342)
Full Name
Rac1/cdc42 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110β (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110β (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA14720)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
IF (Immunofluorescence)
(Figure 11. IF analysis of H Cadherin/CDH13 using anti- H Cadherin/CDH13 antibody (AAA19252).H Cadherin/CDH13 was detected in immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- H Cadherin/CDH13 Antibody (AAA19252) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
H Cadherin/CDH13, Polyclonal Antibody (Cat# AAA19252)
Full Name
Anti-H Cadherin/CDH13 Antibody
Gene Names
CDH13; CDHH; P105
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of CACO-2 cells using anti-PLTP antibody (AAA19255).Overlay histogram showing CACO-2 cells stained with AAA19255 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLTP Antibody (AAA19255, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PLTP, Polyclonal Antibody (Cat# AAA19255)
Full Name
Anti-PLTP Antibody
Gene Names
PLTP; BPIFE; HDLCQ9
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Anti-CD63 Antibody (C-term)at 1:2000 dilution + human plasma lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CD63, Polyclonal Antibody (Cat# AAA28725)
Full Name
CD63 Antibody (C-term)
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing