Search results for " Cell Proliferation Assays"

6 results for " Cell Proliferation Assays" - showing 1-6

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WB (Western Blot) (Western Blot Sample: Recombinant SCF, Porcine; Antibody: Rabbit Anti-Porcine SCF Ab)

SDS-PAGE (Sample: Active recombinant SCF, Porcine)

Identification (Gene Sequence (extract))

Activity (The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 6.07 to 13.69ng/mL.)

Activity (Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37 degree C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1.)

Sequence Information

Stem Cell Factor (SCF), Active Protein (Cat# AAA21108)

Full Name

Active Stem Cell Factor (SCF)

Gene Names

Kitl; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted

Reactivity

Sus scrofa; Porcine (Pig)

Applications

Cell culture; Activity Assays; In vivo assays.

Purity

>95%

Pricing

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WB (Western Blot) (Western BlotSample: Recombinant GAL9, Human;Antibody: Rabbit Anti-Human GAL9 Ab)

SDS-Page (SDS-PAGESample: Active recombinant GAL9, Human)

Application Data (Gene Sequencing (extract))

Application Data (Inhibition of Jurkat cells proliferation after stimulated with GAL9.)

Application Data (GAL9 also can induce cellular apoptosis. To test the effect of GAL9 on cell apoptosis, Jurkat cells were seeded into 96-well plates at a density of 5,000 cells/well with 2% serum standard RPMI 1640 including various concentrations of recombinant human GAL9. After incubated for 48 h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 20 uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 4 h at 37 degree C. Proliferation of Jurkat cells after incubation with GAL9 for 48 h observed by inverted microscope was shown in Figure 3. Cell viability was assessed by CCK-8 assay after incubation with recombinant human GAL9 for 48 h. The result was shown in Figure 4. It was obvious that GAL9 significantly inhibit cell viability of Jurkat cells. The ED50 is 1.64 ug/mL.Inhibition of Jurkat cells proliferation after stimulated with GAL9(A) Jurkat cells cultured in RPMI 1640, stimulated with 2.5ug/mL GAL9 for 48h;(B) Unstimulated Jurkat cells cultured in RPMI 1640 for 48h.)

Application Data (The hemagglutination assay of GAL9 in V- bottom shaped 96-well microtiter plate)

Activity (Galectin 9 (GAL9) is a member of the Beta-galactoside-binding galectin family. Galectin-9 is found outside of cells and may be exported by non-classical pathways. Galectin 9 exhibits a variety of biological activities, the majority of which have focused on its immunomodulatory role toward lymphocytes, were it shows specific interactions with TIM-3, and can negatively regulate Th1 type immunity. It also can agglutinate red blood. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL9 with 0.01M PBS(pH7.4), add 50ul a serial dilution of GAL9 to each well of a U or V- bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL9, replace with 50uL 0.01M PBS. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 1-2 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL9 is 1.5ug/mL.Figure 1.The hemagglutination of recombinant human GAL9(A) Rabbit erythrocyte agglutinated by recombinant human GAL9;(B) Rabbit erythrocyte without recombinant human GAL9.)

Sequence Information

Galectin 9 (GAL9), Active Protein (Cat# AAA21103)

Full Name

Active Galectin 9 (GAL9)

Gene Names

LGALS9; HUAT; LGALS9A

Reactivity

Homo sapiens (Human)

Applications

Cell culture; Activity Assays.

Purity

>90%

Pricing

Standard Curve (Sample)

Interleukin 1beta, IL-1beta, ELISA Kit (Cat# AAA19046)

Full Name

Hamster Interleukin 1beta, IL-1beta ELISA Kit

Gene Names

IL1B; IL1-BETA

Reactivity

Hamster

Pricing

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WB (Western Blot) (Sample: Recombinant GAS6, Human;Antibody: Rabbit Anti-Human GAS6 Ab)

SDS-PAGE (SDS-PAGESample: Active recombinant GAS6, Human)

Identification (Gene Sequencing (extract))

Application Data (The dose-effect curve of GAS6 was shown in Figure 2. It was obvious that GAS6 significantly promoted cell proliferation of DU145 cells. The ED50 for this effect is typically 0.77~38.08ng/mL.)

Activity (Growth arrest-specific 6, also known as GAS6, is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation. It has been reported that both PC-3 and DU 145 human prostate cancer cell lines are stimulated to proliferate by Gas6, however, this proliferative response strictly correlates with the expression of the Axl receptor, being higher in DU 145 cells. To test the proliferative effect of Gas6, DU 145 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of GAS6. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.Cell proliferation of DU145 cells after incubation with GAS6 for 72h observed by inverted microscope was shown in Figure 1.The dose-effect curve of GAS6 was shown in Figure 2. It was obvious that GAS6 significantly promoted cell proliferation of DU145 cells. The ED50 for this effect is typically 0.77~38.08ng/mL.)

Sequence Information

Growth Arrest Specific Protein 6 (GAS6), Active Protein (Cat# AAA21055)

Full Name

Active Growth Arrest Specific Protein 6 (GAS6)

Gene Names

GAS6; AXSF; AXLLG

Reactivity

Homo sapiens (Human)

Applications

Cell Culture, Activity Assay

Purity

>92%

Pricing

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WB (Western Blot) (Sample: Recombinant BMP7, Mouse;Antibody: Rabbit Anti-Mouse BMP7 Ab)

SDS-PAGE (Sample: Active recombinant BMP7, Mouse)

Gene Sequencing (extract)

Application Data (The dose-effect curve of BMP7 was shown in Figure 2. It was obvious that BMP7 significantly promoted cell proliferation of MDA-MB-231 cells. The ED50 for this effect is typically 3.483 to 9.017 ng/mL.)

Activity (BMP7 (Bone morphogenetic protein 7), which belongs to the TGF-beta superfamily, is a signaling molecule with the ability to promote bone formation. BMP7 has also been implicated in various types of cancer, including breast cancer. It has been reported that BMP7 treatment induced cell growth promotion of MDA-MB-231 breast cancer line. To test the effect of BMP7 on cell proliferation, MDA-MB-231 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of BMP7. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.)

Application Data

Bone Morphogenetic Protein 7 (BMP7), Active Protein (Cat# AAA21057)

Full Name

Active Bone Morphogenetic Protein 7 (BMP7)

Gene Names

Bmp7; OP1

Reactivity

Mus musculus (Mouse)

Applications

Cell Culture, Activity Assay

Purity

>98%

Pricing

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Application Data (Staining of human peripheral blood monocytes probed with Mouse anti Human CD14:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 750)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14)

Application Data (Published customer image: CD11b+CD14+MHCII- cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII- cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:Biotin)

Application Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Published customer image: Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14-MHCII- cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14-MHCII- cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14-MHCII- cells is shown at 63x magnification.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14:Pacific Blue)

CD14, Monoclonal Antibody (Cat# AAA11899)

Full Name

MOUSE ANTI HUMAN CD14:Low Endotoxin

Applications

Flow Cytometry, Functional Assay, Western Blot

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6 results for " Cell Proliferation Assays" - showing 1-6

Stem Cell Factor (SCF), Active Protein (Cat# AAA21108)

Galectin 9 (GAL9), Active Protein (Cat# AAA21103)

Interleukin 1beta, IL-1beta, ELISA Kit (Cat# AAA19046)

Growth Arrest Specific Protein 6 (GAS6), Active Protein (Cat# AAA21055)

Bone Morphogenetic Protein 7 (BMP7), Active Protein (Cat# AAA21057)

CD14, Monoclonal Antibody (Cat# AAA11899)

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6 results for " Cell Proliferation Assays" - showing 1-6

Stem Cell Factor (SCF), Active Protein (Cat# AAA21108)

Galectin 9 (GAL9), Active Protein (Cat# AAA21103)

Interleukin 1beta, IL-1beta, ELISA Kit (Cat# AAA19046)

Growth Arrest Specific Protein 6 (GAS6), Active Protein (Cat# AAA21055)

Bone Morphogenetic Protein 7 (BMP7), Active Protein (Cat# AAA21057)

CD14, Monoclonal Antibody (Cat# AAA11899)

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