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9 results for " Caspases" - showing 1-9
FCM (Flow Cytometry)
(Flow cytometric analysis of Pam212 cells, untreated or sulindac sulfone- treated (to induce apoptosis), using AAA14725 Results were similar to those obtained by analyzing DNA content.)
Caspase 3, Cleaved, Polyclonal Antibody (Cat# AAA14725)
Full Name
Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Purified by Protein A and immunoaffinity chromatography.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Cytokeratin 18, Polyclonal Antibody (Cat# AAA31327)
Full Name
Phospho-Cytokeratin 18 (Ser51) Antibody
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using NLRP1 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
NLRP1, Polyclonal Antibody (Cat# AAA28324)
Full Name
NLRP1 Rabbit pAb
Gene Names
NLRP1; NAC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(Published customer imageSLAMF3 blockade in human hepatocytes is associated with lower susceptibility to HCV. (A) SLAMF3 was stained in primary human hepatocytes (PHHs) and cells from the Huh-7 human hepatoma cell line with a specific antibody (HLy9.1.25 clone; grey) and an isotype-matched control (empty). One of four independent experiments is shown. Huh-7 cells were transfected with scrambled control (sc) siRNA or three specific siRNAs (#1, #2 and #3) targeting SLAMF3, prior to infection with HCVcc; siRNA efficiency was checked by quantifying SLAMF3 mRNA (B) and the CD81 expression level (C) by flow cytometry analysis at 48 h post-transfection. Results are presented as the mean +/-SD (n = 3). Intracellular viral RNA was quantified at 72 h p.i. (D) and the infection was measured at 72 h p.i. by focus-forming units FFUs counting (E) (as inhibition percent; mean of three independent experiments; error bars: SD. **p)
CD229, Monoclonal Antibody (Cat# AAA11951)
Full Name
MOUSE ANTI HUMAN CD229
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Applications
Flow Cytometry, Immunoprecipitation
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Bcl-2, Polyclonal Antibody (Cat# AAA31360)
Full Name
Phospho-Bcl-2 (Thr56) Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (86%), Bovine (83%)
Predicted Reactivity: Pig (86%), Bovine (83%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SRPK2, Polyclonal Antibody (Cat# AAA31293)
Full Name
Phospho-SRPK2 (Ser494/Ser497) Antibody
Gene Names
SRPK2; SFRSK2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(THP-I suspension cells were treated with PMA (5 ng/mL) to induce differentiation into macrophages. After 48 hours, PMA was removed and replaced with fresh medium that was spiked with LPS (10 ng/mL) to induce caspase-1 activation. After 2 hours, cells were stained with FAM-YVAD-FMK for 1 hour, washed, and examined under a fluorescence microscope. The top images reveal many experimental cells, several of which fluoresce green, therefore they have active caspase-1. The lower right image reveals many control cells in the field of view, however the corresponding fluorescence image is dark (lower left); few of these cells have active caspase-1. Data courtesy of Dr. Brian Lee, ICT.)
FAM-FLICA Caspase 1, Assay Kit (Cat# AAA12391)
Full Name
FAM-FLICA Caspase 1 Assay Kit
Applications
Flow Cytometry, Fluorescence Microscopy
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Cleaved-Caspase 1, Polyclonal Antibody (Cat# AAA31336)
Full Name
Cleaved-Caspase 1 (Ala317), p10 Antibody
Gene Names
CASP1; ICE; P45; IL1BC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing