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2 results for " Cardiovascular Markers" - showing 1-2
WB (Western Blot)
(Western blot analysis of Podoplanin expression in human placenta lysate (AAA11696).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDPN)
Podoplanin, Monoclonal Antibody (Cat# AAA11696)
Full Name
Anti-Podoplanin Rabbit Monoclonal Antibody tested for WB in Human, Mouse, Rat.
Gene Names
PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of parafffin-embedded rat spleen using Lysozyme Polyclonal Antibody at dilution of 1:400.)
Lysozyme, Polyclonal Antibody (Cat# AAA22122)
Full Name
Lysozyme Polyclonal Antibody
Gene Names
Lyz2; Lys; Lzm; Lzp; Lysm; Lyzs; Lyzf2; Lzm-s1; AI326280
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing