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27 results for " Agarose" - showing 1-27
IP (Immunoprecipitation)
(CD147 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-CD147 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 50 kDa)
CD147, Monoclonal Antibody (Cat# AAA27726)
Full Name
Recombinant Anti-CD147 Antibody, Rabbit Monoclonal
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
Application Data
(Purified rabbit igG was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP secondary antibody and chemiluminescent detection.)
Goat anti Rabbit IgG, Polyclonal Secondary Antibody (Cat# AAA14909)
Full Name
Goat Anti-Rabbit IgG-UNLB
Reactivity
Rabbit
Applications
Immunoblot, Immunofluorescence, Immunohistochemistry
Purity
Affinity chromatography on rabbit IgG covalently linked to agarose
Pricing
Application Data
(FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-PE)
Goat Anti-Rat IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14908)
Full Name
Goat Anti-Rat IgG-HRP
Reactivity
Rat IgM; may react with immunoglobulins from other species.
Applications
ELISA
Purity
Affinity chromatography on pooled rat IgG covalently linked to agarose
Pricing
Application Data
(ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-HRP.)
Goat Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14904)
Full Name
Goat Anti-Human IgG-HRP
Applications
ELISA
Purity
Affinity chromatography on human IgG covalently linked to agarose
Pricing
WB (Western Blot)
(Western blot analysis of A431cells treated with calyculin A (10 nM) for 30 min. The blot was probed with four different rabbit polyclonal antibodies (lane 1: anti-Akt (Thr-34), lane 2: anti-β-Catenin, lane 3: anti-N-WASP (Ser-484/Ser-485), lane 4: anti-Paxillin (Ser-178). This panel of primary antibodies was then detected using goat anti-rabbit Ig:HRP (1:5,000).)
Ig, Polyclonal Secondary Antibody (Cat# AAA14101)
Full Name
Anti-Rabbit Ig:HRP Secondary Antibody
Reactivity
Rabbit
Applications
Western Blot
Pricing
WB (Western Blot)
(Anti-TRIM28 rabbit polyclonal antibody at 1:500 dilution Lane A: TRIM28 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:89 kDa Observed band size:115 kDa (We are unsure as to the identity of these extra bands.))
KAP1/TRIM28, Polyclonal Antibody (Cat# AAA27715)
Full Name
Anti-KAP1/TRIM28 Antibody, Rabbit Polyclonal
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(HSPA1A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-HSPA1A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HSPA1A rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 70 kDaObserved band size: 70 kDa)
HSP70, Monoclonal Antibody (Cat# AAA27747)
Full Name
Recombinant Anti-HSP70 Antibody, Rabbit Monoclonal
Gene Names
HSPA1B; HSP72; HSPA1; HSX70; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1B
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
WB (Western Blot)
(Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: ANP32A konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)
ANP32A, Polyclonal Antibody (Cat# AAA27748)
Full Name
Anti-ANP32A Antibody, Rabbit Polyclonal
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistchemistry)
(Immunochemical staining CD21 in cynomolgus lymph node with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
CD21, Polyclonal Antibody (Cat# AAA27739)
Full Name
Anti-CD21 Antibody, Rabbit Polyclonal
Gene Names
CR2; CR; C3DR; CD21; CVID7; SLEB9
Reactivity
Human, Cynomolgus
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-PARP1 mouse monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:113 kDaObserved band size:113 kDa)
PARP, Monoclonal Antibody (Cat# AAA27741)
Full Name
Anti-PARP Antibody, Mouse Monoclonal
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A
Pricing
IP (Immunoprecipitation)
(HDAC2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate4 uL anti-HDAC2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HDAC2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)
HDAC2, Polyclonal Antibody (Cat# AAA27718)
Full Name
Anti-HDAC2 Antibody, Rabbit Polyclonal
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-GP73 rabbit polyclonal antibody at 1:500 dilution Lane A: GOLM1 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size: kDa)
GOLPH2/GOLM1, Polyclonal Antibody (Cat# AAA27751)
Full Name
Anti-GOLPH2/GOLM1 Antibody, Rabbit Polyclonal
Gene Names
GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry)
(Immunochemical staining HMGB1 in rat liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
HMGB1, Polyclonal Antibody (Cat# AAA27719)
Full Name
Anti-HMGB1 Antibody, Rabbit Polyclonal
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(LAMP1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-LAMP1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LAMP1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 113 kDa)
LAMP1, Monoclonal Antibody (Cat# AAA27745)
Full Name
Recombinant Anti-LAMP1 Antibody, Rabbit Monoclonal
Gene Names
LAMP1; LAMPA; CD107a; LGP120
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-EpCAM rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)
EpCAM, Monoclonal Antibody (Cat# AAA27736)
Full Name
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
Activity
Proteinase K (PROK), Active Protein (Cat# AAA27052)
Full Name
Recombinant Tritirachium album Proteinase K (PROK) (Active)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
IP (Immunoprecipitation)
(Hspb7 was immunoprecipitated using: Lane A:0.5 mg NIH-3T3 Whole Cell Lysate Lane B:0.5 mg A549 Whole Cell Lysate 4 uL anti-Hspb7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Hspb7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 19 kDa Observed band size: 22 kDa)
HSPB7, Polyclonal Antibody (Cat# AAA27732)
Full Name
Anti-HSPB7 Antibody, Rabbit Polyclonal
Gene Names
Hspb7; 27kDa; cvHsp; Hsp25-2
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
Application Data
(Detect RNase residue by agarose gel electrophores)
Proteinase K-lyophilization, Protein (Cat# AAA28067)
Full Name
Proteinase K-lyophilization
Purity
>=95% by SDS-PAGE
Pricing
Application Data
(Source:Arthrobacter luteus)
Zymolyase 20T, Enzyme (Cat# AAA14844)
Full Name
Zymolyase 20T (Lyticase, Yeast Lytic Enzyme)
Pricing
Goat Anti-Human IgG, Monkey ads, Secondary Antibody (Cat# AAA14913)
Full Name
Goat Anti-Human IgG, Monkey ads-UNLB
Applications
ELISA, FLISA
Purity
Affinity chromatography on human IgG covalently linked to agarose
Pricing
IgE, Monoclonal Native Protein (Cat# AAA12300)
Full Name
RAT IgE KAPPA
Gene Names
Ighe; IGH2
Applications
ELISA
Purity
Single band by agarose eletrophoresis (5) and immunoelectrophoresis (6)
Pricing
Streptokinase, Active Protein (Cat# AAA10820)
Full Name
Recombinant Streptokinase
Purity
Greather than 97% as determined by:
(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE
(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE
Pricing
Application Data
c-Src (Tyr-215), Polyclonal Antibody (Cat# AAA14100)
Full Name
c-Src (Tyr-215), phospho-specific [Conserved site]
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, Rat, Mouse
Applications
Western Blot
Pricing
Goat Anti-Human IgG (γ chain specific), Secondary Antibody (Cat# AAA14903)
Full Name
Goat Anti-Human IgG-AP
Reactivity
Human
Applications
Flow Cytometry
Purity
Affinity chromatography on human IgG covalently linked to agarose
Pricing
IgG, Polyclonal Secondary Antibody (Cat# AAA30581)
Full Name
Goat Anti-Mouse IgG (H&L) HRP
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Pricing
IP (Immunoprecipitation)
(IP antibody use: 5ug VSV-G Mouse IgG1 per ml Lysate, WB 1:20001, untransfected 293 cell lysate 2, transfected 293 cell lysate with VSV-G-tag fusion protein3, IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4, IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5, IP (transfected 293+anti-VSV-G mAb+ Protein G agarose)6, IP (transfected 293+Protein G)7, Recombinant protein (E.coli))
VSV-G-Tag, Monoclonal Antibody (Cat# AAA27915)
Full Name
VSV-G-Tag Mouse Monoclonal Antibody
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Pricing
ELISA
(ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-BIOT (AAA14911) followed by Streptavidin-HRP (please inquire).)
Goat Anti-Human IgG, Monkey ads, Secondary Antibody (Cat# AAA14911)
Full Name
Goat Anti-Human IgG, Monkey ads-BIOT
Reactivity
Human
Applications
ELISA, FLISA
Purity
Affinity chromatography on Human IgG covalently linked to agarose.
Pricing