Search results for " Abs to HLA"

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA14785)

Full Name

b2-Microglobulin (B2M, Beta-2-microglobulin, CDABP0092, HDCMA22P)

Gene Names

HLA-G; MHC-G

Reactivity

Human

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

HLA Class 1 Antigen A24,A11,A2403, Monoclonal Antibody (Cat# AAA14669)

Full Name

HLA Class 1 Antigen A24,A11,A2403 (X-Reactive)

Reactivity

Human

Applications

Flow Cytometry

Purity

Ascites

Pricing

HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive), Monoclonal Antibody (Cat# AAA14681)

Full Name

HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive)

Reactivity

Human

Applications

Cell Typing

Purity

Ascites

Pricing

HLA Class 1 Antigen A11, Monoclonal Antibody (Cat# AAA14666)

Full Name

Anti-Human HLA Class 1 Antigen A11 (Biotin)

Applications

Flow Cytometry

Purity

Purified by Ion Exchange chromatography

Pricing

HLA Class 1 Antigen A11, Monoclonal Antibody (Cat# AAA14664)

Full Name

HLA Class 1 Antigen A11

Reactivity

Human

Applications

Flow Cytometry

Purity

Ascites

Pricing

HLA Class 1 Antigen A1,A11,A26, Monoclonal Antibody (Cat# AAA14657)

Full Name

HLA Class 1 Antigen A1,A11,A26 (X-Reactive)

Applications

Cell Typing

Purity

Ascites

Pricing

HLA Class 1 Antigen A30,A31, Monoclonal Antibody (Cat# AAA14671)

Full Name

HLA Class 1 Antigen A30,A31 (Biotin)

Gene Names

HLA-A; HLAA

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified by Ion Exchange chromatography.

Pricing

HLA Class 1 Antigen A1,A36, Monoclonal Antibody (Cat# AAA14672)

Full Name

HLA Class 1 Antigen A1,A36 (Biotin)

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified by Ion Exchange chromatography.

Pricing

HLA Class 1 Antigen A25,A26, Monoclonal Antibody (Cat# AAA14658)

Full Name

HLA Class 1 Antigen A25,A26 (Biotin)

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence

Purity

> 98%, Purified by Ion Exchange chromatography.

Pricing

b2-Microglobulin, Monoclonal Antibody (Cat# AAA14654)

Full Name

b2-Microglobulin (B2M)

Gene Names

HLA-G; MHC-G

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence

Purity

Purified

Pricing

HLA Class 1 Antigen B17,B35,B44(X-Reactive), Monoclonal Antibody (Cat# AAA14659)

Full Name

HLA Class 1 Antigen B17,B35,B44(X-Reactive)

Reactivity

Human

Purity

Ascites

Pricing

< >

<< Previous Next >>

Application Data (Mouse anti Human HLA DP/DQ/DR antibody, clone WR18 used for the evaluation of HLA class II histocompatibility antigen expression on cell populations by flow cytometry.Image caption:DC differentiation from H1 hESC.Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.From: Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ.Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012;2012:172420.doi: 10.1155/2012/172420.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the treatment on monocytes in vitrodetection of MHC class II onImage caption:Ligand engagement of HLA class II molecules up regulates MyD88, IL-1R1, and TNF-? in CD14+ human monocytes treated with SEB, SEA, or mAb directed against MHC-class II molecules. (A) Transcriptional activation of MyD88, IL-1R1AcP and TNF-? in HLA-DR positive primary monocytes treated with 200 ng SEB/ml (optimum dose), mAbs (10 µg/ml, optimum dose) directed against MHC-class II molecule (anti-DR-DP-DQ or LB3.1) or unrelated control antibody OKT3 was examined by semi-quantitative RT-PCR. Data shown is one of 3 similar experiments; (B) Agonists binding to TLR4 or HLA class II molecules on CD14+ monocytes induced transcriptional up regulation of MyD88. Real time RT-PCR was used to determine relative expression of MyD88 normalized to the expression of ?-actin. Expression levels are determined as means ± SD. Data presented as one of 3 similar experiments. Significance compared to untreated control (*) was assigned as P?0.0001. (C) MHC class II molecule dependence of SEB- induced TNF- ? gene expression. Pretreatment of monocytes in ice with anti-DR-DP-DQ at optimum dose (10 ug/ml) followed by SEB stimulation resulted in reduced TNF- ? gene expression. Transcriptional activation of TNF- ? expression normalized to the expression of ?-actin. Expression levels of TNF- ? are expressed as means ± SD. Significance was assigned (*) or (**) as P values ?0.003 comparing untreated vs treatment groups with anti-DR-DP-DQ, SEB or SEB vs anti-DR-DP-DQ+SEB respectively. (D) Confocal images show expression of HLA-DR (green) and intracellular MyD88 (red) proteins in CD14+ monocytes treated with HLA class II- ligands or control antibody OKT3 for 16 h; Scale bar = 5 ?m; (E) intracellular expression of MyD88 protein in activated monocytes. Primary monocytes (CD14+, CD3-) were activated as described earlier, permeabilized and labeled with primary MyD88 antibody followed by PE-labeled secondary antibody and analyzed by flow cytometry. Histogram represents a MHC class II ligand-induced increase in expression of MyD88 protein compared to non-MHC class II ligand (OKT3).From: Kissner TL, Ruthel G, Alam S, Ulrich RG, Fernandez S, et al. (2011) Activation of MyD88 Signaling upon Staphylococcal Enterotoxin Binding to MHC Class II Molecules.PLoS ONE 6(1): e15985.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the evaluation of MHC class II expression on dendritic cells by flow cytometry.Image caption:Time course of DC differentiation from H1 hESC. Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (a) Cells harvested at day 20 of culture showing expressing of CD45 but lack of myeloid commitment, as evidenced by staining for CD13, CD14, and CD11c. Open histograms show levels of background staining using isotype-matched control antibodies. (b) Appearance of CD45int cells at day 27 of culture, accompanied by the upregulation of myeloid-specific markers. (c) Photomicrograph, taken at day 28 of culture, showing the morphology of DC, including veils of cytoplasm and long dendrites (inset) (×40 magnification). (d) Cells harvested at day 33 of culture, showing the appearance of a CD45hi population containing predominantly DC progenitors expressing CD14, CD11c, CD86 and MHC class I. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.From: Kathryn M. Silk, Alison J. Leishman, Kevin P. Nishimoto, Anita Reddy, and Paul J. Fairchild, “Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype,”Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 172420, 11 pages, 2012.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

Full Name

Mouse Anti Human HLA DP DQ DR: Amethyst Orange

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Pricing

HLA Class 1 Antigen A23,A24, Monoclonal Antibody (Cat# AAA14677)

Full Name

HLA Class 1 Antigen A23,A24

Reactivity

Human

Applications

Cell Typing

Purity

Ascites

Pricing

HLA Class 1 Antigen A33,B8, Monoclonal Antibody (Cat# AAA14684)

Full Name

Mouse anti-Human HLA Class 1 Antigen A33,B8

Reactivity

Human

Applications

Cell Typing

Purity

Ascites

Pricing

HLA Class 1 Antigen A30,A31, Monoclonal Antibody (Cat# AAA14656)

Full Name

Mouse Anti-Human HLA Class 1 Antigen A30,A31 (R0.87)

Gene Names

HLA-A; HLAA

Reactivity

Human

Applications

Cell Typing

Purity

Ascites

Pricing

Loading...

Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

15 results for " Abs to HLA" - showing 1-15

b2-Microglobulin, Monoclonal Antibody (Cat# AAA14785)

HLA Class 1 Antigen A24,A11,A2403, Monoclonal Antibody (Cat# AAA14669)

HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive), Monoclonal Antibody (Cat# AAA14681)

HLA Class 1 Antigen A11, Monoclonal Antibody (Cat# AAA14666)

HLA Class 1 Antigen A11, Monoclonal Antibody (Cat# AAA14664)

HLA Class 1 Antigen A1,A11,A26, Monoclonal Antibody (Cat# AAA14657)

HLA Class 1 Antigen A30,A31, Monoclonal Antibody (Cat# AAA14671)

HLA Class 1 Antigen A1,A36, Monoclonal Antibody (Cat# AAA14672)

HLA Class 1 Antigen A25,A26, Monoclonal Antibody (Cat# AAA14658)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA14654)

HLA Class 1 Antigen B17,B35,B44(X-Reactive), Monoclonal Antibody (Cat# AAA14659)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

HLA Class 1 Antigen A23,A24, Monoclonal Antibody (Cat# AAA14677)

HLA Class 1 Antigen A33,B8, Monoclonal Antibody (Cat# AAA14684)

HLA Class 1 Antigen A30,A31, Monoclonal Antibody (Cat# AAA14656)

Loading...

Need a datasheet, manual, COA, or MSDS? Have a question?

Interested to get a quote about our products?

Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

15 results for " Abs to HLA" - showing 1-15

b2-Microglobulin, Monoclonal Antibody (Cat# AAA14785)

HLA Class 1 Antigen A24,A11,A2403, Monoclonal Antibody (Cat# AAA14669)

HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive), Monoclonal Antibody (Cat# AAA14681)

HLA Class 1 Antigen A11, Monoclonal Antibody (Cat# AAA14666)

HLA Class 1 Antigen A11, Monoclonal Antibody (Cat# AAA14664)

HLA Class 1 Antigen A1,A11,A26, Monoclonal Antibody (Cat# AAA14657)

HLA Class 1 Antigen A30,A31, Monoclonal Antibody (Cat# AAA14671)

HLA Class 1 Antigen A1,A36, Monoclonal Antibody (Cat# AAA14672)

HLA Class 1 Antigen A25,A26, Monoclonal Antibody (Cat# AAA14658)

b2-Microglobulin, Monoclonal Antibody (Cat# AAA14654)

HLA Class 1 Antigen B17,B35,B44(X-Reactive), Monoclonal Antibody (Cat# AAA14659)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

HLA Class 1 Antigen A23,A24, Monoclonal Antibody (Cat# AAA14677)

HLA Class 1 Antigen A33,B8, Monoclonal Antibody (Cat# AAA14684)

HLA Class 1 Antigen A30,A31, Monoclonal Antibody (Cat# AAA14656)

Submit a Question

Request more Information

Request a Manual

Request a Quote