Search results for " Abs to Binding Proteins"

7 results for " Abs to Binding Proteins" - showing 1-7

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Application Data (S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)

S100A8, Monoclonal Antibody (Cat# AAA14670)

Full Name

S100A8 (MRP-8)

Gene Names

S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8

Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Purity

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

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WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

ARID3A, Monoclonal Antibody (Cat# AAA14788)

Full Name

ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B-cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1)

Gene Names

ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1

Reactivity

Human

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (37kD) .)

RBM9, Monoclonal Antibody (Cat# AAA14778)

Full Name

RBM9 (RNA Binding Motif Protein 9, RNA Binding Protein 9, Hexaribonucleotide Binding Protein 2, HRNBP2, Repressor of Tamoxifen Transcriptional Activity, RTA)

Reactivity

Human, Mouse

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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DDX41, Polyclonal Antibody (Cat# AAA23454)

Full Name

DDX41 antibody - N-terminal region

Gene Names

DDX41; ABS; MPLPF

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Purity

Affinity Purified

Pricing

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WB (Western Blot) (ACBD3 monoclonal antibody. Western Blot analysis of ACBD3 expression in PC-12.)

ACBD3, Monoclonal Antibody (Cat# AAA24049)

Full Name

ACBD3 (Golgi Resident Protein GCP60, Acyl-CoA-binding Domain-containing Protein 3, Golgi Complex-associated Protein 1, GOCAP1, Golgi Phosphoprotein 1, GOLPH1, PBR- and PKA-associated Protein 7, Peripheral Benzodiazepine Receptor-associated Protein PAP7, G

Gene Names

ACBD3; PAP7; GCP60; GOCAP1; GOLPH1

Reactivity

Human, Mouse, Rat

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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Application Data (Mouse anti Human HLA DP/DQ/DR antibody, clone WR18 used for the evaluation of HLA class II histocompatibility antigen expression on cell populations by flow cytometry.Image caption:DC differentiation from H1 hESC.Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.From: Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ.Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012;2012:172420.doi: 10.1155/2012/172420.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the treatment on monocytes in vitrodetection of MHC class II onImage caption:Ligand engagement of HLA class II molecules up regulates MyD88, IL-1R1, and TNF-? in CD14+ human monocytes treated with SEB, SEA, or mAb directed against MHC-class II molecules. (A) Transcriptional activation of MyD88, IL-1R1AcP and TNF-? in HLA-DR positive primary monocytes treated with 200 ng SEB/ml (optimum dose), mAbs (10 µg/ml, optimum dose) directed against MHC-class II molecule (anti-DR-DP-DQ or LB3.1) or unrelated control antibody OKT3 was examined by semi-quantitative RT-PCR. Data shown is one of 3 similar experiments; (B) Agonists binding to TLR4 or HLA class II molecules on CD14+ monocytes induced transcriptional up regulation of MyD88. Real time RT-PCR was used to determine relative expression of MyD88 normalized to the expression of ?-actin. Expression levels are determined as means ± SD. Data presented as one of 3 similar experiments. Significance compared to untreated control (*) was assigned as P?0.0001. (C) MHC class II molecule dependence of SEB- induced TNF- ? gene expression. Pretreatment of monocytes in ice with anti-DR-DP-DQ at optimum dose (10 ug/ml) followed by SEB stimulation resulted in reduced TNF- ? gene expression. Transcriptional activation of TNF- ? expression normalized to the expression of ?-actin. Expression levels of TNF- ? are expressed as means ± SD. Significance was assigned (*) or (**) as P values ?0.003 comparing untreated vs treatment groups with anti-DR-DP-DQ, SEB or SEB vs anti-DR-DP-DQ+SEB respectively. (D) Confocal images show expression of HLA-DR (green) and intracellular MyD88 (red) proteins in CD14+ monocytes treated with HLA class II- ligands or control antibody OKT3 for 16 h; Scale bar = 5 ?m; (E) intracellular expression of MyD88 protein in activated monocytes. Primary monocytes (CD14+, CD3-) were activated as described earlier, permeabilized and labeled with primary MyD88 antibody followed by PE-labeled secondary antibody and analyzed by flow cytometry. Histogram represents a MHC class II ligand-induced increase in expression of MyD88 protein compared to non-MHC class II ligand (OKT3).From: Kissner TL, Ruthel G, Alam S, Ulrich RG, Fernandez S, et al. (2011) Activation of MyD88 Signaling upon Staphylococcal Enterotoxin Binding to MHC Class II Molecules.PLoS ONE 6(1): e15985.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Mouse anti Human HLA DP DQ DR antibody, clone WR18 used for the evaluation of MHC class II expression on dendritic cells by flow cytometry.Image caption:Time course of DC differentiation from H1 hESC. Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (a) Cells harvested at day 20 of culture showing expressing of CD45 but lack of myeloid commitment, as evidenced by staining for CD13, CD14, and CD11c. Open histograms show levels of background staining using isotype-matched control antibodies. (b) Appearance of CD45int cells at day 27 of culture, accompanied by the upregulation of myeloid-specific markers. (c) Photomicrograph, taken at day 28 of culture, showing the morphology of DC, including veils of cytoplasm and long dendrites (inset) (×40 magnification). (d) Cells harvested at day 33 of culture, showing the appearance of a CD45hi population containing predominantly DC progenitors expressing CD14, CD11c, CD86 and MHC class I. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.From: Kathryn M. Silk, Alison J. Leishman, Kevin P. Nishimoto, Anita Reddy, and Paul J. Fairchild, “Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype,”Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 172420, 11 pages, 2012.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

Full Name

Mouse Anti Human HLA DP DQ DR: Amethyst Orange

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Pricing

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FCM (Flow Cytometry) (Flow Cytometry detection of FOLR1 in MCF-7 human cell line using AAA14691. MCF-7 human breast cancer cell line was stained with AAA14691 (filled histogram) or isotype control antibody (open histogram), followed byPhycoerythrin-conjugated Anti-MouseIgG Secondary Antibody.)

FOLR1, Monoclonal Antibody (Cat# AAA14691)

Full Name

FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)

Gene Names

FOLR1; FBP; FOLR

Reactivity

Human

Applications

Flow Cytometry, Western Blot, Immunocytochemistry

Purity

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Pricing

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7 results for " Abs to Binding Proteins" - showing 1-7

S100A8, Monoclonal Antibody (Cat# AAA14670)

ARID3A, Monoclonal Antibody (Cat# AAA14788)

RBM9, Monoclonal Antibody (Cat# AAA14778)

DDX41, Polyclonal Antibody (Cat# AAA23454)

ACBD3, Monoclonal Antibody (Cat# AAA24049)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

FOLR1, Monoclonal Antibody (Cat# AAA14691)

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7 results for " Abs to Binding Proteins" - showing 1-7

S100A8, Monoclonal Antibody (Cat# AAA14670)

ARID3A, Monoclonal Antibody (Cat# AAA14788)

RBM9, Monoclonal Antibody (Cat# AAA14778)

DDX41, Polyclonal Antibody (Cat# AAA23454)

ACBD3, Monoclonal Antibody (Cat# AAA24049)

HLA DP DQ DR, Monoclonal Antibody (Cat# AAA12274)

FOLR1, Monoclonal Antibody (Cat# AAA14691)

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