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FCM/FACS (Flow Cytometry)
(AAA60839 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Goat STAT3 Polyclonal Antibody | anti-STAT3 antibody
Goat anti-STAT3 (isoform 1 and 2) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
STAT3, Antibody; Goat anti-STAT3 (isoform 1 and 2) Antibody; Goat Anti-STAT3 Antibody; STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); APRF; DNA-binding protein APRF; acute-phase response factor; FLJ20882; MGC16063; HIES; STAT3 antibody; signal transducer and activator of transcription 3 (acute-phase response factor) antibody; APRF antibody; DNA-binding protein APRF antibody; acute-phase response factor antibody; FLJ20882 antibody; MGC16063 antibody; HIES antibody; STAT3 (isoform 1 and 2); anti-STAT3 antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence
DMELTSECATSPM
Sequence Length
769
Applicable Applications for anti-STAT3 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2; NP_644805.1.
Epitope
C Terminus
Note
This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
FCM/FACS (Flow Cytometry)
(AAA60839 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(AAA60839 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(AAA60839 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA60839 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA60839 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and weak nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA60839 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and weak nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IHC (Immunohiostchemistry)
(AAA60839 Negative Control showing staining of paraffin embedded Mouse Thymus, with no primary antibody)
IHC (Immunohiostchemistry)
(AAA60839 Negative Control showing staining of paraffin embedded Mouse Thymus, with no primary antibody)
IHC (Immunohistochemistry)
(AAA60839 (4ug/ml) staining of paraffin embedded Mouse Thymus. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
(AAA60839 (4ug/ml) staining of paraffin embedded Mouse Thymus. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
signal transducer and activator of transcription 3 isoform 2
NCBI Official Synonym Full Names
signal transducer and activator of transcription 3
NCBI Official Symbol
STAT3
NCBI Official Synonym Symbols
APRF; HIES; ADMIO
NCBI Protein Information
signal transducer and activator of transcription 3
UniProt Protein Name
Signal transducer and activator of transcription 3
UniProt Gene Name
STAT3
UniProt Entry Name
STAT3_HUMAN
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Product Notes
The STAT3 stat3 (Catalog #AAA60839) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-STAT3 (isoform 1 and 2) Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's STAT3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the STAT3 stat3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DMELTSECAT SPM. It is sometimes possible for the material contained within the vial of "STAT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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