FCM/FACS (Flow Cytometry)
(SPINK5 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human SPINK5 Polyclonal Antibody | anti-SPINK5 antibody
SPINK5 Antibody (N-term)
Gene Names
SPINK5; NS; NETS; LEKTI; LETKI; VAKTI
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SPINK5, Antibody; SPINK5 Antibody (N-term); Serine protease inhibitor Kazal-type 5; Lympho-epithelial Kazal-type-related inhibitor; LEKTI; Hemofiltrate peptide HF6478; Hemofiltrate peptide HF7665; SPINK5; anti-SPINK5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SPINK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the N-terminal region of human SPINK5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
188-217
Sequence Length
1094
Applicable Applications for anti-SPINK5 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(SPINK5 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(SPINK5 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(SPINK5 Antibody (N-term) (RB18954) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPINK5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(SPINK5 Antibody (N-term) (RB18954) IHC analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPINK5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of SPINK5 Antibody (N-term) in A2058 cell line lysates (35ug/lane). SPINK5 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of SPINK5 Antibody (N-term) in A2058 cell line lysates (35ug/lane). SPINK5 (arrow) was detected using the purified Pab.)
Related Product Information for anti-SPINK5 antibody
SPINK5 is a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The inhibitor may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy.
Product Categories/Family for anti-SPINK5 antibody
References
Nin,M., et.al., J. Dermatol. Sci. 54 (1), 17-24 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120714
NCBI Official Full Name
serine protease inhibitor Kazal-type 5 isoform a
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kazal type 5
NCBI Official Symbol
SPINK5
NCBI Official Synonym Symbols
NS; NETS; LEKTI; LETKI; VAKTI
NCBI Protein Information
serine protease inhibitor Kazal-type 5
UniProt Protein Name
Serine protease inhibitor Kazal-type 5
UniProt Gene Name
SPINK5
UniProt Synonym Gene Names
LEKTI
UniProt Entry Name
ISK5_HUMAN
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Product Notes
The SPINK5 spink5 (Catalog #AAA285798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 188-217. The SPINK5 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPINK5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SPINK5 spink5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPINK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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