IP (Immunoprecipitation)
(MAPK8 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate4 uL anti-MAPK8 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAPK8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa)
Rabbit anti-Human JNK1 Polyclonal Antibody | anti-MAPK8 antibody
Anti-JNK1 Antibody, Rabbit Polyclonal
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
JNK1, Antibody; Anti-JNK1 Antibody, Rabbit Polyclonal; JNK1/MAPK8 Antibody, Rabbit PAb, Antigen Affinity Purified; mitogen-activated protein kinase 8; Anti-JNK Antibody; Anti-JNK-46 Antibody; Anti-JNK1 Antibody; Anti-JNK1A2 Antibody; Anti-JNK21B1/2 Antibody; Anti-PRKM8 Antibody; Anti-SAPK1 Antibody; Anti-SAPK1c Antibody; anti-MAPK8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human JNK1/MAPK8
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MAPK8 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human JNK1/MAPK8 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human JNK1/MAPK8 (rh JNK1/MAPK8). JNK1/MAPK8 specific IgG was purified by Human JNK1/MAPK8 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(MAPK8 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate4 uL anti-MAPK8 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAPK8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa)
IP (Immunoprecipitation)
(MAPK8 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate4 uL anti-MAPK8 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MAPK8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of MAPK8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MAPK8 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of MAPK8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MAPK8 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
WB (Western Blot)
(Anti-MAPK8 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-MAPK8 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-MAPK8 antibody
Mitogen-activated protein kinase 8 (MAPK8), also known as JNK1, is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. The protein kinases JNK1 has been found to serve as critical molecular links between obesity, metabolic inflammation, and disorders of glucose homeostasis. It is critically involved in the promotion of diet-induced obesity, metabolic inflammation, and beta-cell dysfunction. The selective deficiency of JNK1 in the murine nervous system is sufficient to suppress diet-induced obesity. Genetic analysis indicates that the effects of JNK1 can be separated from the effects of JNK1 on obesity. JNK1 is a potential pharmacological target for the development of drugs that might be useful for the treatment of metabolic syndrome, and type 2 diabetes. Furthermore, JNK1 plays a major role in hypoxic cellular damage. JNK1 protein might be an attractive target for anti-hypoxic therapy in increasing resistance to many pathological conditions and diseases, leading to the oxygen deficit.
Product Categories/Family for anti-MAPK8 antibody
References
Betigeri S, et al. (2006) JNK1 as a molecular target to limit cellular mortality under hypoxia. Mol Pharm. 3(4): 424-30.Solinas G, et al. (2010) JNK1 and IKKbeta: molecular links between obesity and metabolic dysfunction. FASEB J. 24(8): 2596-611.Sabio G, et al. (2010) Role of the hypothalamic-pituitary-thyroid axis in metabolic regulation by JNK1. Genes Dev. 24(3): 256-64.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,333 Da
NCBI Official Full Name
mitogen-activated protein kinase 8 isoform alpha2
NCBI Official Synonym Full Names
mitogen-activated protein kinase 8
NCBI Official Symbol
MAPK8
NCBI Official Synonym Symbols
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
NCBI Protein Information
mitogen-activated protein kinase 8; MAP kinase 8; JUN N-terminal kinase; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kin
UniProt Protein Name
Mitogen-activated protein kinase 8
UniProt Gene Name
MAPK8
UniProt Synonym Gene Names
JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c
UniProt Entry Name
MK08_HUMAN
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Product Notes
The MAPK8 mapk8 (Catalog #AAA255166) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-JNK1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JNK1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAPK8 mapk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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