Rabbit FUS Polyclonal Antibody | anti-FUS antibody

FUS Antibody (C-term)

Cat. Num. AAA283899

• Gene Names: FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• Reactivity: Human, Mouse; Predicted: Bovine
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FUS, Antibody; FUS Antibody (C-term); RNA-binding protein FUS; 75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein; FUS; TLS; anti-FUS antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Bovine
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FUS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499-526 amino acids from the C-terminal region of human FUS.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 499-526

• Applicable Applications for anti-FUS antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response (PubMed: ). Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing (PubMed: ). Binds also its own pre- mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay (PubMed: ). Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair (PubMed: ). In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (By similarity).
• Cellular Location: Nucleus Note=Displays a punctate pattern inside the nucleus and is excluded from nucleoli.
• Tissue Location: Ubiquitous.
• Antigen Region: 499-526
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of FUS Antibody (C-term) (AAA283899) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

FCM/FACS (Flow Cytometry)

(FUS Antibody (C-term) (AAA283899) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(FUS antibody (C-term) (AAA283899) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUS antibody (C-term) (AAA283899) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(FUS Antibody (C-term) (AAA283899) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the FUS antibody detected FUS protein (arrow).)

Related Product Information for anti-FUS antibody

This gene encodes a multifunctional protein component of
the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The
hnRNP complex is involved in pre-mRNA splicing and the export of
fully processed mRNA to the cytoplasm. This protein belongs to the
FET family of RNA-binding proteins which have been implicated in
cellular processes that include regulation of gene expression,
maintenance of genomic integrity and mRNA/microRNA processing.
Alternative splicing results in multiple transcript variants.
Defects in this gene result in amyotrophic lateral sclerosis type 6.

Product Categories/Family for anti-FUS antibody

Cancer

References

Kim, S.H., et al. J. Biol. Chem. 285(44):34097-34105(2010)
Mackenzie, I.R., et al. Lancet Neurol 9(10):995-1007(2010)
Yan, J., et al. Neurology 75(9):807-814(2010)
Waibel, S., et al. Neurology 75(9):815-817(2010)
Baumer, D., et al. Neurology 75(7):611-618(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 283135201
• NCBI GeneID: 2521
• NCBI Accession #: NP_001164105.1; NP_004951.1
• NCBI GenBank Nucleotide #: NM_001170634.1
• UniProt Accession #: P35637; Q9H4A8
• Molecular Weight: 53426
• NCBI Official Full Name: RNA-binding protein FUS isoform 2
• NCBI Official Synonym Full Names: FUS RNA binding protein
• NCBI Official Symbol: FUS
• NCBI Official Synonym Symbols: TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• NCBI Protein Information: RNA-binding protein FUS
• UniProt Protein Name: RNA-binding protein FUS
• UniProt Gene Name: FUS
• UniProt Synonym Gene Names: TLS
• UniProt Entry Name: FUS_HUMAN