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product-image-AAA286039_WB13.jpg WB (Western Blot) (F9 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the F9 antibody detected the F9 protein (arrow).)

Rabbit IgG anti-Human F9 Polyclonal Antibody | anti-F9 antibody

F9 Antibody (Center)

Gene Names
F9; FIX; P19; PTC; HEMB; THPH8
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
F9, Antibody; F9 Antibody (Center); Coagulation factor IX; Christmas factor; Plasma thromboplastin component; PTC; Coagulation factor IXa light chain; Coagulation factor IXa heavy chain; F9; anti-F9 antibody
Ordering
Host
Rabbit IgG
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This F9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human F9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
266-295
Sequence Length
461
Applicable Applications for anti-F9 antibody
ELISA, WB (Western Blot)
Cellular Location
Secreted
Tissue Location
Detected in blood plasma (at protein level) (PubMed:3857619, PubMed:8295821, PubMed:2592373, PubMed:9169594, PubMed:19846852). Synthesized primarily in the liver and secreted in plasma.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(F9 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the F9 antibody detected the F9 protein (arrow).)

product-image-AAA286039_WB13.jpg WB (Western Blot) (F9 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the F9 antibody detected the F9 protein (arrow).)

WB (Western Blot)

(All lanes : Anti-F9 Antibody (Center) at 1:2000 dilution Lane 1: 293T/17 whole cell lysateLane 2: A431 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

product-image-AAA286039_WB15.jpg WB (Western Blot) (All lanes : Anti-F9 Antibody (Center) at 1:2000 dilution Lane 1: 293T/17 whole cell lysateLane 2: A431 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-F9 antibody
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Product Categories/Family for anti-F9 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Yang, L., et al. J. Biol. Chem. 285(37):28488-28495(2010)
Kao, C.Y., et al. Thromb. Haemost. 104(2):355-365(2010)
Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
Arellano, A.R., et al. J. Thromb. Haemost. 8(5):1132-1134(2010)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51778
NCBI Official Full Name
coagulation factor IX preproprotein
NCBI Official Synonym Full Names
coagulation factor IX
NCBI Official Symbol
F9
NCBI Official Synonym Symbols
FIX; P19; PTC; HEMB; THPH8
NCBI Protein Information
coagulation factor IX
UniProt Protein Name
Coagulation factor IX
UniProt Gene Name
F9
UniProt Synonym Gene Names
PTC
UniProt Entry Name
FA9_HUMAN

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Product Notes

The F9 f9 (Catalog #AAA286039) is an Antibody produced from Rabbit IgG and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 266-295. The F9 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's F9 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the F9 f9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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