Sheep EGFR Polyclonal Antibody | anti-EGFR antibody

Sheep Polyclonal (IgG) to Human EGFR

Cat. Num. AAA50860

• Gene Names: EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• Reactivity: Chicken, Human, Mouse, Rat
• Applications: ELISA, Immunoprecipitation, Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: Protein G Purified
• Synonyms: EGFR, Antibody; Sheep Polyclonal (IgG) to Human EGFR; Anti-EGFR Antibody (Internal) IHC-plus; EGFR; EGFf receptor; ERBB; ERBB1; MENA; Panitumumab; PIG61; Proto-oncogene c-ErbB-1; V-erb-b homolog; EGF-R; HER1; Human EGFR; anti-EGFR antibody

• Host: Sheep
• Reactivity: Chicken, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Epidermal Growth Factor Receptor.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.4. No preservatives added.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1210

• Applicable Applications for anti-EGFR antibody: ELISA, IP (Immunoprecipitation), WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
• Immunogen Species: EGFR antibody was raised against Human
• Immunogen Type: Recombinant protein
• Immunogen: EGFR antibody was raised against recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

IHC (Immunohistochemistry)

(Anti-EGFR antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-EGFR antibody

Epidermal growth factor receptor (EGFR) is a tyrosine protein kinase comprised of two different components of different molecular weights. Both components contain phosphotyrosine and phosphothreonine but only the higher molecular weight component contains phosphoserine. EGFR signaling involves small GTPases of the Rho family and EGFR trafficking involves small GTPases of the Rab family. EGFR expression is widespread in both normal and cancerous tissues.

Product Categories/Family for anti-EGFR antibody

Family: Protein Kinase; Subfamily: EGF Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 29725609
• NCBI GeneID: 1956
• NCBI Accession #: NP_005219.2
• NCBI GenBank Nucleotide #: NM_005228.3
• UniProt Accession #: P00533; O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7
• Molecular Weight: 69,228 Da
• NCBI Official Full Name: epidermal growth factor receptor isoform a
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• NCBI Protein Information: epidermal growth factor receptor; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: ERBB; ERBB1; HER1
• UniProt Entry Name: EGFR_HUMAN