IHC (Immunohistochemisry)
(EEF1A1 Antibody (C-term) (AAA285859)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit EEF1A1 Polyclonal Antibody | anti-EEF1A1 antibody
EEF1A1 Antibody (C-term)
Gene Names
EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
Reactivity
Reactivity: HumanPredicted Reactivity: Xenopus, Bovine, Hamster, Horse, Mouse, Rabbit, Rat, Chicken, Zebrafish
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot, Immunofluorescence
Synonyms
EEF1A1, Antibody; EEF1A1 Antibody (C-term); Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryoticelongation factor 1 A-1, eEF1A-1, Leukocytereceptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7; anti-EEF1A1 antibody
Host
Rabbit
Reactivity
Reactivity: Human
Predicted Reactivity: Xenopus, Bovine, Hamster, Horse, Mouse, Rabbit, Rat, Chicken, Zebrafish
Predicted Reactivity: Xenopus, Bovine, Hamster, Horse, Mouse, Rabbit, Rat, Chicken, Zebrafish
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibodyis prepared by Saturated Ammonium Sulfate(SAS) precipitation followed by dialysis against PBS.
Sequence Positions
361-389
Applicable Applications for anti-EEF1A1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence)
Function
This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed: ).
Cellular Location
Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane. Note=Colocalizes with DLC1 atactin-rich regions in the cell periphery (PubMed: 19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed: 8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed: 26497934).
Tissue Location
Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(EEF1A1 Antibody (C-term) (AAA285859)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(EEF1A1 Antibody (C-term) (AAA285859)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EEF1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of EEF1A1 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of EEF1A1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of EEF1A1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-EEF1A1 antibody
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
Product Categories/Family for anti-EEF1A1 antibody
References
Byun,H.O., Cancer Res. 69 (11), 4638-4647 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 50141
NCBI Official Full Name
elongation factor 1-alpha 1
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 1
NCBI Official Symbol
EEF1A1
NCBI Official Synonym Symbols
CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
NCBI Protein Information
elongation factor 1-alpha 1
UniProt Protein Name
Elongation factor 1-alpha 1
UniProt Gene Name
EEF1A1
UniProt Synonym Gene Names
EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1
UniProt Entry Name
EF1A1_HUMAN
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Product Notes
The EEF1A1 eef1a1 (Catalog #AAA285859) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 361-389. The EEF1A1 Antibody (C-term) reacts with Reactivity: Human Predicted Reactivity: Xenopus, Bovine, Hamster, Horse, Mouse, Rabbit, Rat, Chicken, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1A1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the EEF1A1 eef1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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