FCM/FACS (Flow Cytometry)
(CCSF1R Antibody (Cat. # AAA284935) flowcytometric analysis of k562 cells (bottomhistogram) compared to a negative control(top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CSF1R Polyclonal Antibody | anti-CSF1R antibody
CSF1R Antibody
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence
Synonyms
CSF1R, Antibody; CSF1R Antibody; Macrophage colony-stimulating factor 1receptor, CSF-1 receptor, CSF-1-R, CSF-1R,M-CSF-R, Proto-oncogene c-Fms, CD115,CSF1R, FMS; anti-CSF1R antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CSF1R antibody is generated from rabbits immunized with CSF1R recombinant protein.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-CSF1R antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence)
Cellular Location
Cell membrane; Single-pass type Imembrane protein
Tissue Location
Expressed in bone marrow and indifferentiated blood mononuclear cells
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CCSF1R Antibody (Cat. # AAA284935) flowcytometric analysis of k562 cells (bottomhistogram) compared to a negative control(top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CCSF1R Antibody (Cat. # AAA284935) flowcytometric analysis of k562 cells (bottomhistogram) compared to a negative control(top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CSF1R Antibody(Cat# AAA284935) with HepG2 cell followed by Alexa Fluor 488-conjugatedgoat anti-rabbit lgG (green).DAPI was used tostain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CSF1R Antibody(Cat# AAA284935) with HepG2 cell followed by Alexa Fluor 488-conjugatedgoat anti-rabbit lgG (green).DAPI was used tostain the cell nuclear (blue).)
WB (Western Blot)
(Anti-CSF1R Antibody at 1:2000 dilution +HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CSF1R Antibody at 1:2000 dilution +HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-CSF1R antibody
Background:
CSF1R is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine.Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is atyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases.
Function:
Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation ofsurvival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such asmacrophages and monocytes. Promotes there lease of proinflammatory chemokines inresponse to IL34 and CSF1, and there by plays an important role in innate immunity and in inflammatory processes. Plays animportant role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male andfemale fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway(PubMed: 20504948,PubMed: 30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacyl glycerol and inositol1, 4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase,leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteinsthat directly interact with phosphorylatedtyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2.Promotes activation of STAT familymembers STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases,such as INPP5D/SHIP-1, thatdephosphorylate the receptor and itsdownstream effectors, and by rapidinternalization of the activated receptor. Inthe central nervous system, may play a rolein the development of microgliamacrophages (PubMed: 30982608).
CSF1R is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine.Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is atyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases.
Function:
Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation ofsurvival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such asmacrophages and monocytes. Promotes there lease of proinflammatory chemokines inresponse to IL34 and CSF1, and there by plays an important role in innate immunity and in inflammatory processes. Plays animportant role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male andfemale fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway(PubMed: 20504948,PubMed: 30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacyl glycerol and inositol1, 4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase,leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteinsthat directly interact with phosphorylatedtyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2.Promotes activation of STAT familymembers STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases,such as INPP5D/SHIP-1, thatdephosphorylate the receptor and itsdownstream effectors, and by rapidinternalization of the activated receptor. Inthe central nervous system, may play a rolein the development of microgliamacrophages (PubMed: 30982608).
Product Categories/Family for anti-CSF1R antibody
References
Guey,L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010)
Wooten,E.C., et.al., PLoS ONE 5 (1), E8830 (2010)
Hosgood,H.D. III, et.al., Respir Med 103 (12), 1866-1870 (2009)
Wooten,E.C., et.al., PLoS ONE 5 (1), E8830 (2010)
Hosgood,H.D. III, et.al., Respir Med 103 (12), 1866-1870 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
macrophage colony-stimulating factor 1 receptor
NCBI Official Synonym Full Names
colony stimulating factor 1 receptor
NCBI Official Symbol
CSF1R
NCBI Official Synonym Symbols
FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
NCBI Protein Information
macrophage colony-stimulating factor 1 receptor
UniProt Protein Name
Macrophage colony-stimulating factor 1 receptor
UniProt Gene Name
CSF1R
UniProt Synonym Gene Names
FMS; CSF-1-R; CSF-1R; M-CSF-R
UniProt Entry Name
CSF1R_HUMAN
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Product Notes
The CSF1R csf1r (Catalog #AAA284935) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CSF1R Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CSF1R can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the CSF1R csf1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSF1R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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